ID   GADW_SHIFL              Reviewed;         242 AA.
AC   P63203; P37638;
DT   27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2004, sequence version 1.
DT   25-MAY-2022, entry version 111.
DE   RecName: Full=HTH-type transcriptional regulator GadW;
GN   Name=gadW; OrderedLocusNames=SF3596, S4171;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Depending on the conditions (growth phase and medium), acts
CC       as a positive or negative regulator of gadA and gadBC. Repression
CC       occurs directly or via the repression of the expression of gadX.
CC       Activation occurs directly by the binding of GadW to the gadA and gadBC
CC       promoters (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- INDUCTION: Expression can be repressed by GadX, depending on the
CC       conditions. {ECO:0000250}.
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DR   EMBL; AE005674; AAN45047.1; -; Genomic_DNA.
DR   EMBL; AE014073; AAP19140.1; -; Genomic_DNA.
DR   RefSeq; NP_709340.1; NC_004337.2.
DR   RefSeq; WP_000149999.1; NZ_WPGW01000173.1.
DR   AlphaFoldDB; P63203; -.
DR   STRING; 198214.SF3596; -.
DR   EnsemblBacteria; AAN45047; AAN45047; SF3596.
DR   EnsemblBacteria; AAP19140; AAP19140; S4171.
DR   GeneID; 1026315; -.
DR   GeneID; 58391716; -.
DR   KEGG; sfl:SF3596; -.
DR   KEGG; sfx:S4171; -.
DR   PATRIC; fig|198214.7.peg.4246; -.
DR   HOGENOM; CLU_000445_81_4_6; -.
DR   OMA; EIDECIV; -.
DR   OrthoDB; 1268974at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR018060; HTH_AraC.
DR   InterPro; IPR018062; HTH_AraC-typ_CS.
DR   InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR   Pfam; PF12833; HTH_18; 1.
DR   PRINTS; PR00032; HTHARAC.
DR   SMART; SM00342; HTH_ARAC; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR   PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..242
FT                   /note="HTH-type transcriptional regulator GadW"
FT                   /id="PRO_0000194517"
FT   DOMAIN          139..236
FT                   /note="HTH araC/xylS-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        156..177
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        203..226
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
SQ   SEQUENCE   242 AA;  28028 MW;  40C11B74DA2F8DA5 CRC64;
     MTHVCSVILI RRSFDIYHEQ QKISLHNESI LLLEKNLADD FAFCSPDTRR LDIDELTVCH
     YLQNIRQLPR NLGLHSKDRL LINQSPPMPL VTAIFDSFNE SGVNSPILSN MLYLSCLSMF
     SHKKELIPLL FNSISTVSGK VERLISFDIA KRWYLRDIAE RMYTSESLIK KKLQDENTCF
     SKILLASRMS MARRLLELRQ IPLHTIAEKC GYSSTSYFIN TFRQYYGVTP HQFAQHSPGT
     FS