ID   GADX_ECO27              Reviewed;         274 AA.
AC   Q9EYV5; B7UL69;
DT   14-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 106.
DE   RecName: Full=HTH-type transcriptional regulator GadX;
GN   Name=gadX; OrderedLocusNames=E2348C_3758;
OS   Escherichia coli O127:H6 (strain E2348/69 / EPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=574521;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX   PubMed=11555293; DOI=10.1046/j.1365-2958.2001.02570.x;
RA   Shin S., Castanie-Cornet M.-P., Foster J.W., Crawford J.A., Brinkley C.,
RA   Kaper J.B.;
RT   "An activator of glutamate decarboxylase genes regulates the expression of
RT   enteropathogenic Escherichia coli virulence genes through control of the
RT   plasmid-encoded regulator, Per.";
RL   Mol. Microbiol. 41:1133-1150(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=E2348/69 / EPEC;
RX   PubMed=18952797; DOI=10.1128/jb.01238-08;
RA   Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T., Henderson I.R.,
RA   Harris D., Asadulghani M., Kurokawa K., Dean P., Kenny B., Quail M.A.,
RA   Thurston S., Dougan G., Hayashi T., Parkhill J., Frankel G.;
RT   "Complete genome sequence and comparative genome analysis of
RT   enteropathogenic Escherichia coli O127:H6 strain E2348/69.";
RL   J. Bacteriol. 191:347-354(2009).
CC   -!- FUNCTION: Positively regulates the expression of about fifteen genes
CC       involved in acid resistance such as gadA, gadB and gadC. Depending on
CC       the conditions (growth phase and medium), can repress gadW (By
CC       similarity). Negatively regulates perA expression in acidic conditions
CC       and positively regulates it in alkaline conditions. {ECO:0000250,
CC       ECO:0000269|PubMed:11555293}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- INDUCTION: Expression can be activated by RpoS and repressed by CRP, H-
CC       NS and GadW, depending on the conditions. {ECO:0000250}.
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DR   EMBL; AF311305; AAG42472.1; -; Genomic_DNA.
DR   EMBL; FM180568; CAS11306.1; -; Genomic_DNA.
DR   RefSeq; WP_001182739.1; NC_011601.1.
DR   AlphaFoldDB; Q9EYV5; -.
DR   SMR; Q9EYV5; -.
DR   EnsemblBacteria; CAS11306; CAS11306; E2348C_3758.
DR   KEGG; ecg:E2348C_3758; -.
DR   HOGENOM; CLU_091292_0_0_6; -.
DR   OMA; NIANDWS; -.
DR   Proteomes; UP000008205; Chromosome.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR018060; HTH_AraC.
DR   InterPro; IPR018062; HTH_AraC-typ_CS.
DR   InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR   Pfam; PF12833; HTH_18; 1.
DR   PRINTS; PR00032; HTHARAC.
DR   SMART; SM00342; HTH_ARAC; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR   PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Repressor; Transcription; Transcription regulation;
KW   Virulence.
FT   CHAIN           1..274
FT                   /note="HTH-type transcriptional regulator GadX"
FT                   /id="PRO_0000194519"
FT   DOMAIN          145..242
FT                   /note="HTH araC/xylS-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        162..183
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        209..232
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
SQ   SEQUENCE   274 AA;  31289 MW;  1495ACED6B8FC18F CRC64;
     MQPLHGNCLI AYARHKYILT MVNGEYRYFN GGDLVFADAS QIQVDKCVEN FVLVSRDTLS
     LFLPMLKEEA LKLHAHKKVP SLLVHHCTRD IPVFQEVAQL SQNKNLRYAE MLRKRALIFA
     LLSVFLEDTQ FIPLLLNVLQ PNMRTRVCTV INNNIAHEWT LARIASELLM SPSLLKKKLR
     EEGTSYSQLL TECRMQRALQ LIVIYGVSIK RVAVSCGYHS VSYFIYVFRN YYGMTPTEYQ
     ERSAQELPNC GPAASMAAQG NFYGTDRSAE GIRL