ID   GADX_ECO57              Reviewed;         274 AA.
AC   P58230;
DT   14-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT   14-AUG-2001, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=HTH-type transcriptional regulator GadX;
GN   Name=gadX; OrderedLocusNames=Z4929, ECs4396;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Positively regulates the expression of about fifteen genes
CC       involved in acid resistance such as gadA, gadB and gadC. Depending on
CC       the conditions (growth phase and medium), can repress gadW (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- INDUCTION: Expression can be activated by RpoS and repressed by CRP, H-
CC       NS and GadW, depending on the conditions. {ECO:0000250}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE005174; AAG58657.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB37819.1; -; Genomic_DNA.
DR   PIR; D91178; D91178.
DR   PIR; E86024; E86024.
DR   RefSeq; NP_312423.1; NC_002695.1.
DR   RefSeq; WP_001191074.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P58230; -.
DR   SMR; P58230; -.
DR   STRING; 155864.EDL933_4767; -.
DR   EnsemblBacteria; AAG58657; AAG58657; Z4929.
DR   EnsemblBacteria; BAB37819; BAB37819; ECs_4396.
DR   GeneID; 915747; -.
DR   KEGG; ece:Z4929; -.
DR   KEGG; ecs:ECs_4396; -.
DR   PATRIC; fig|386585.9.peg.4595; -.
DR   eggNOG; COG2207; Bacteria.
DR   HOGENOM; CLU_091292_0_0_6; -.
DR   OMA; NIANDWS; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR018060; HTH_AraC.
DR   InterPro; IPR018062; HTH_AraC-typ_CS.
DR   InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR   Pfam; PF12833; HTH_18; 1.
DR   PRINTS; PR00032; HTHARAC.
DR   SMART; SM00342; HTH_ARAC; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR   PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..274
FT                   /note="HTH-type transcriptional regulator GadX"
FT                   /id="PRO_0000194520"
FT   DOMAIN          145..242
FT                   /note="HTH araC/xylS-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        162..183
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        209..232
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
SQ   SEQUENCE   274 AA;  31372 MW;  1C2A626CE6886CDD CRC64;
     MQSLHGNCLI AYARHKYILT MVNGEYRYFN GGDLVFADAS QIRVDKCVEN FVLVSRDTLS
     LFLPMLKEEA LNLHAHKKVS SLLVHHCSRD IPVFQEVAQL SQNKNLRYAE MLRKRALIFA
     LLSVFLEDDH FIPLLLNVLQ PNMRTRVCTV INNNIAHEWT LARIASELLM SPSLLKKKLR
     EEETSYSQLL TECRMQRALQ LIVIHGFSIK RVAVSCGYHS VSYFIYVFRN YYGMTPTEYQ
     ERSAQGLPNR DSAASIVAQG NFYGTDCSAE GIRL