GADX_ECOLI
ID GADX_ECOLI Reviewed; 274 AA.
AC P37639; Q2M7H8;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=HTH-type transcriptional regulator GadX;
GN Name=gadX; Synonyms=yhiX; OrderedLocusNames=b3516, JW3484;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION, AND GENE NAME.
RC STRAIN=FB8, and K12 / MG1655 / ATCC 47076;
RX PubMed=11298273; DOI=10.1046/j.1365-2958.2001.02358.x;
RA Hommais F., Krin E., Laurent-Winter C., Soutourina O., Malpertuy A.,
RA Le Caer J.-P., Danchin A., Bertin P.;
RT "Large-scale monitoring of pleiotropic regulation of gene expression by the
RT prokaryotic nucleoid-associated protein, H-NS.";
RL Mol. Microbiol. 40:20-36(2001).
RN [5]
RP FUNCTION, AND INDUCTION.
RC STRAIN=ATCC 11246;
RX PubMed=11976288; DOI=10.1128/jb.184.10.2603-2613.2002;
RA Tramonti A., Visca P., De Canio M., Falconi M., De Biase D.;
RT "Functional characterization and regulation of gadX, a gene encoding an
RT AraC/XylS-like transcriptional activator of the Escherichia coli glutamic
RT acid decarboxylase system.";
RL J. Bacteriol. 184:2603-2613(2002).
RN [6]
RP FUNCTION, INDUCTION, AND SUBUNIT.
RC STRAIN=K12;
RX PubMed=12446650; DOI=10.1128/jb.184.24.7001-7012.2002;
RA Ma Z., Richard H., Tucker D.L., Conway T., Foster J.W.;
RT "Collaborative regulation of Escherichia coli glutamate-dependent acid
RT resistance by two AraC-like regulators, GadX and GadW (YhiW).";
RL J. Bacteriol. 184:7001-7012(2002).
RN [7]
RP FUNCTION, AND INDUCTION.
RC STRAIN=K12;
RX PubMed=12730179; DOI=10.1128/jb.185.10.3190-3201.2003;
RA Tucker D.L., Tucker N., Ma Z., Foster J.W., Miranda R.L., Cohen P.S.,
RA Conway T.;
RT "Genes of the GadX-GadW regulon in Escherichia coli.";
RL J. Bacteriol. 185:3190-3201(2003).
RN [8]
RP FUNCTION.
RX PubMed=14617649; DOI=10.1128/jb.185.23.6852-6859.2003;
RA Ma Z., Richard H., Foster J.W.;
RT "pH-dependent modulation of cyclic AMP levels and GadW-dependent repression
RT of RpoS affect synthesis of the GadX regulator and Escherichia coli acid
RT resistance.";
RL J. Bacteriol. 185:6852-6859(2003).
RN [9]
RP FUNCTION.
RC STRAIN=FB8;
RX PubMed=14702398; DOI=10.1099/mic.0.26659-0;
RA Hommais F., Krin E., Coppee J.-Y., Lacroix C., Yeramian E., Danchin A.,
RA Bertin P.;
RT "GadE (YhiE): a novel activator involved in the response to acid
RT environment in Escherichia coli.";
RL Microbiology 150:61-72(2004).
CC -!- FUNCTION: Positively regulates the expression of about fifteen genes
CC involved in acid resistance such as gadA, gadB and gadC. Depending on
CC the conditions (growth phase and medium), can repress gadW.
CC {ECO:0000269|PubMed:11298273, ECO:0000269|PubMed:11976288,
CC ECO:0000269|PubMed:12446650, ECO:0000269|PubMed:12730179,
CC ECO:0000269|PubMed:14617649, ECO:0000269|PubMed:14702398}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:12446650}.
CC -!- INDUCTION: Expression can be activated by RpoS and repressed by CRP, H-
CC NS and GadW, depending on the conditions. {ECO:0000269|PubMed:11976288,
CC ECO:0000269|PubMed:12446650, ECO:0000269|PubMed:12730179}.
CC -!- MISCELLANEOUS: GadX and GadW are part of a complex system required for
CC acid resistance via the regulation of gadA and gadBC. Many of the
CC described circuits use mutants missing one of the network components.
CC In the wild-type situation, however, many of the scenarios are not
CC easily observed.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U00039; AAB18492.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76541.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77778.1; -; Genomic_DNA.
DR PIR; S47736; S47736.
DR RefSeq; NP_417973.1; NC_000913.3.
DR RefSeq; WP_001191059.1; NZ_SSZK01000042.1.
DR PDB; 3MKL; X-ray; 2.15 A; A/B=137-255.
DR PDBsum; 3MKL; -.
DR AlphaFoldDB; P37639; -.
DR SMR; P37639; -.
DR BioGRID; 4262524; 14.
DR IntAct; P37639; 6.
DR STRING; 511145.b3516; -.
DR jPOST; P37639; -.
DR PaxDb; P37639; -.
DR PRIDE; P37639; -.
DR EnsemblBacteria; AAC76541; AAC76541; b3516.
DR EnsemblBacteria; BAE77778; BAE77778; BAE77778.
DR GeneID; 948028; -.
DR KEGG; ecj:JW3484; -.
DR KEGG; eco:b3516; -.
DR PATRIC; fig|1411691.4.peg.3202; -.
DR EchoBASE; EB2154; -.
DR eggNOG; COG2207; Bacteria.
DR HOGENOM; CLU_091292_0_0_6; -.
DR InParanoid; P37639; -.
DR OMA; NIANDWS; -.
DR PhylomeDB; P37639; -.
DR BioCyc; EcoCyc:EG12243-MON; -.
DR PRO; PR:P37639; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IDA:EcoCyc.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IMP:EcoCyc.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IEP:EcoliWiki.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:EcoCyc.
DR GO; GO:0006351; P:transcription, DNA-templated; IDA:EcoCyc.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR018060; HTH_AraC.
DR InterPro; IPR018062; HTH_AraC-typ_CS.
DR InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR Pfam; PF12833; HTH_18; 1.
DR PRINTS; PR00032; HTHARAC.
DR SMART; SM00342; HTH_ARAC; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; DNA-binding; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..274
FT /note="HTH-type transcriptional regulator GadX"
FT /id="PRO_0000194518"
FT DOMAIN 145..242
FT /note="HTH araC/xylS-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT DNA_BIND 162..183
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT DNA_BIND 209..232
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT HELIX 143..152
FT /evidence="ECO:0007829|PDB:3MKL"
FT HELIX 161..167
FT /evidence="ECO:0007829|PDB:3MKL"
FT HELIX 172..181
FT /evidence="ECO:0007829|PDB:3MKL"
FT HELIX 186..202
FT /evidence="ECO:0007829|PDB:3MKL"
FT HELIX 209..215
FT /evidence="ECO:0007829|PDB:3MKL"
FT HELIX 221..232
FT /evidence="ECO:0007829|PDB:3MKL"
FT HELIX 236..242
FT /evidence="ECO:0007829|PDB:3MKL"
SQ SEQUENCE 274 AA; 31563 MW; C5B742F296436DC8 CRC64;
MQSLHGNCLI AYARHKYILT MVNGEYRYFN GGDLVFADAS QIRVDKCVEN FVFVSRDTLS
LFLPMLKEEA LNLHAHKKVS SLLVHHCSRD IPVFQEVAQL SQNKNLRYAE MLRKRALIFA
LLSVFLEDEH FIPLLLNVLQ PNMRTRVCTV INNNIAHEWT LARIASELLM SPSLLKKKLR
EEETSYSQLL TECRMQRALQ LIVIHGFSIK RVAVSCGYHS VSYFIYVFRN YYGMTPTEYQ
ERSAQRLSNR DSAASIVAQG NFYGTDRSAE GIRL
