ID   GADX_SHIFL              Reviewed;         274 AA.
AC   Q83PR0; Q7BYZ7;
DT   27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 114.
DE   RecName: Full=HTH-type transcriptional regulator GadX;
GN   Name=gadX; OrderedLocusNames=SF3595, S4172;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Positively regulates the expression of about fifteen genes
CC       involved in acid resistance such as gadA, gadB and gadC. Depending on
CC       the conditions (growth phase and medium), can repress gadW (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- INDUCTION: Expression can be activated by RpoS and repressed by CRP, H-
CC       NS and GadW, depending on the conditions. {ECO:0000250}.
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DR   EMBL; AE005674; AAN45046.1; -; Genomic_DNA.
DR   EMBL; AE014073; AAP19141.1; -; Genomic_DNA.
DR   RefSeq; NP_709339.1; NC_004337.2.
DR   RefSeq; WP_001191070.1; NZ_WPGW01000173.1.
DR   AlphaFoldDB; Q83PR0; -.
DR   SMR; Q83PR0; -.
DR   STRING; 198214.SF3595; -.
DR   EnsemblBacteria; AAN45046; AAN45046; SF3595.
DR   EnsemblBacteria; AAP19141; AAP19141; S4172.
DR   GeneID; 1026323; -.
DR   GeneID; 58391717; -.
DR   KEGG; sfl:SF3595; -.
DR   KEGG; sfx:S4172; -.
DR   PATRIC; fig|198214.7.peg.4244; -.
DR   HOGENOM; CLU_091292_0_0_6; -.
DR   OMA; NIANDWS; -.
DR   OrthoDB; 1268974at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR018060; HTH_AraC.
DR   InterPro; IPR018062; HTH_AraC-typ_CS.
DR   InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR   Pfam; PF12833; HTH_18; 1.
DR   PRINTS; PR00032; HTHARAC.
DR   SMART; SM00342; HTH_ARAC; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR   PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..274
FT                   /note="HTH-type transcriptional regulator GadX"
FT                   /id="PRO_0000194522"
FT   DOMAIN          145..242
FT                   /note="HTH araC/xylS-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        162..183
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        209..232
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
SQ   SEQUENCE   274 AA;  31452 MW;  76EAB1A51C62476F CRC64;
     MQSLHGNCLI AYARHKYILT MVNGEYRYFN GGDLVFADAS QIRVDKCVEN FVLVSRDTLS
     LFLPMLKEEA LNLHAHKKIS SLLVHHCSRD IPVFQEVAQL SQNKNLRYAE MLRKRALIFA
     LLSVFLEDEH FIPLLLNVLQ PNMRTRVCTV INNNIAHEWT LARIASELLM SPSLLKKKLR
     EEETSYSQLL TECRMQRALQ LIVIHGFSIK RVAVSCGYHS VSYFIYVFRN YYGMTPTEYQ
     ERSAQGLPNR DSAASIVAQG NFYGTNRSAE GIRL