GAF1_ARATH
ID GAF1_ARATH Reviewed; 581 AA.
AC F4JXF1; Q9FJD9;
DT 13-NOV-2019, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Protein GAMETOPHYTE DEFECTIVE 1 {ECO:0000303|PubMed:22509260};
DE AltName: Full=Probable ribonuclease P protein subunit p30 {ECO:0000303|PubMed:22509260};
DE Short=AtRPP30 {ECO:0000303|PubMed:22509260};
DE Short=RNaseP protein p30 {ECO:0000303|PubMed:22509260};
DE EC=3.1.26.- {ECO:0000250|UniProtKB:P78346};
GN Name=GAF1 {ECO:0000303|PubMed:22509260};
GN Synonyms=RPP30 {ECO:0000303|PubMed:22509260};
GN OrderedLocusNames=At5g59980 {ECO:0000312|Araport:AT5G59980};
GN ORFNames=MMN10.23 {ECO:0000312|EMBL:BAB08366.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT features of the regions of 1,013,767 bp covered by sixteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:297-308(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH POP5, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, AND SUBCELLULAR LOCATION.
RX PubMed=22509260; DOI=10.1371/journal.pone.0033595;
RA Wang S.-Q., Shi D.-Q., Long Y.-P., Liu J., Yang W.-C.;
RT "GAMETOPHYTE DEFECTIVE 1, a putative subunit of RNases P/MRP, is essential
RT for female gametogenesis and male competence in Arabidopsis.";
RL PLoS ONE 7:E33595-E33595(2012).
CC -!- FUNCTION: Probable component of ribonuclease P, a ribonucleoprotein
CC complex that generates mature tRNA molecules by cleaving their 5'-ends
CC (By similarity). May also be a component of the MRP ribonuclease
CC complex, which cleaves pre-rRNA sequences (By similarity). Required for
CC female gametophyte development and male competence (PubMed:22509260).
CC {ECO:0000250|UniProtKB:P78346, ECO:0000269|PubMed:22509260}.
CC -!- SUBUNIT: Probable component of nuclear RNase P and RNase MRP
CC ribonucleoproteins (By similarity). Interacts with POP5
CC (PubMed:22509260). {ECO:0000250|UniProtKB:P78346,
CC ECO:0000269|PubMed:22509260}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000250|UniProtKB:P38786}. Nucleus {ECO:0000269|PubMed:22509260}.
CC Mitochondrion {ECO:0000269|PubMed:22509260}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in inflorescence and roots, to a
CC lower extent in leaves, and, at low levels, in siliques, seedlings and
CC stems. {ECO:0000269|PubMed:22509260}.
CC -!- DEVELOPMENTAL STAGE: Expressed in seedlings, young leaves, root tips
CC and lateral primordia (PubMed:22509260). In reproductive organs,
CC present in the inflorescence, especially in pollen grains, nucellar
CC cells and embryo sacs (PubMed:22509260). {ECO:0000269|PubMed:22509260}.
CC -!- DISRUPTION PHENOTYPE: Impaired progression of the gametophytic division
CC during female gametogenesis leading to arrested embryo sacs at stages
CC ranging from FG1 to FG7 (PubMed:22509260). Normal pollen development,
CC but weaker pollen tube fitness associated with a reduced transmission
CC through the male gametes (PubMed:22509260).
CC {ECO:0000269|PubMed:22509260}.
CC -!- SIMILARITY: Belongs to the eukaryotic/archaeal RNase P protein
CC component 3 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB08366.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB015475; BAB08366.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED97261.1; -; Genomic_DNA.
DR RefSeq; NP_200806.2; NM_125391.3.
DR AlphaFoldDB; F4JXF1; -.
DR SMR; F4JXF1; -.
DR STRING; 3702.AT5G59980.2; -.
DR PRIDE; F4JXF1; -.
DR ProteomicsDB; 204175; -.
DR EnsemblPlants; AT5G59980.1; AT5G59980.1; AT5G59980.
DR GeneID; 836120; -.
DR Gramene; AT5G59980.1; AT5G59980.1; AT5G59980.
DR KEGG; ath:AT5G59980; -.
DR Araport; AT5G59980; -.
DR HOGENOM; CLU_023213_0_0_1; -.
DR PRO; PR:F4JXF1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; F4JXF1; baseline and differential.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR InterPro; IPR002738; RNase_P_p30.
DR PANTHER; PTHR13031; PTHR13031; 1.
DR Pfam; PF01876; RNase_P_p30; 1.
DR SUPFAM; SSF89550; SSF89550; 1.
PE 1: Evidence at protein level;
KW Hydrolase; Mitochondrion; Nucleus; Reference proteome; rRNA processing;
KW tRNA processing.
FT CHAIN 1..581
FT /note="Protein GAMETOPHYTE DEFECTIVE 1"
FT /id="PRO_0000448555"
FT REGION 452..512
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 452..482
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 492..512
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 581 AA; 64079 MW; 631D96866F1AFF2D CRC64;
MGFFDLSIPY NEPPRSGGKE IAGGKTLRLK LATKAMELGY VGIAHNRSIK GVMSDKDSCT
IPLLTLGSLI KVAPRLASSV GFHRDLLGVP RTTPFRQYTR LTVHVESNAQ CQSLNSGNPI
LKSYDIIAVR PMNQNAFDYA CEKAEVDLIS IDFTDKMLFR LKHPMVKAAI QRGIYFEIKY
SDILMDAQTR RQVISNAKLL VDWTRGKNLI ISSGAPSVTE LRGPNDVINL MFLLGLSAER
ARAAISKNCR NMIAKVLKKK RFHKEAVRVE LLSAGDTFSL EQPLSEDCMK WDRLSSGEGD
MLLDDLAKAF DATNVVAHKS SKAIDFTSVL DGLPKHGFRV KDIVGTESVT QPSAAKVIDT
QVHSSNQVSE LRMATASSDD NLREIETISQ IDMLMSEDDN KVEPTTNVLK EEAFALRKCS
ASHGQGILVQ NQTATPFTLT RCTKSEAASD VSMNIESTSE GGSMSPSKSD HGIPQSPVEV
NNMGNAAFEE EASVDENSKE RATTGHASND EMHITESGHH ASIDDEKHIP EPEHLTSIAD
EMKIDCSSEA NHDEYMEVTM EDQMHETVQM RLCKTMTKHQ D
