GAF1_SCHPO
ID GAF1_SCHPO Reviewed; 855 AA.
AC Q10280; O94482; Q9USK9;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Transcription factor gaf1;
DE Short=Gaf-1;
GN Name=gaf1; ORFNames=SPCC1902.01, SPCC417.01c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 566-855.
RX PubMed=9714831; DOI=10.1016/s0378-1119(98)00301-1;
RA Hoe K.-L., Won M.S., Chung K.-S., Park S.-K., Kim D.-U., Jang Y.-J.,
RA Yoo O.-J., Yoo H.-S.;
RT "Molecular cloning of gaf1, a Schizosaccharomyces pombe GATA factor, which
RT can function as a transcriptional activator.";
RL Gene 215:319-328(1998).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-150; SER-727 AND SER-729, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- FUNCTION: Transcriptional activator.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
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DR EMBL; CU329672; CAB40003.1; -; Genomic_DNA.
DR EMBL; L31601; AAC35593.1; -; Genomic_DNA.
DR PIR; T41336; T41336.
DR RefSeq; NP_588278.2; NM_001023268.2.
DR AlphaFoldDB; Q10280; -.
DR SMR; Q10280; -.
DR BioGRID; 275597; 4.
DR STRING; 4896.SPCC1902.01.1; -.
DR iPTMnet; Q10280; -.
DR MaxQB; Q10280; -.
DR PaxDb; Q10280; -.
DR PRIDE; Q10280; -.
DR EnsemblFungi; SPCC1902.01.1; SPCC1902.01.1:pep; SPCC1902.01.
DR GeneID; 2539024; -.
DR KEGG; spo:SPCC1902.01; -.
DR PomBase; SPCC1902.01; gaf1.
DR VEuPathDB; FungiDB:SPCC1902.01; -.
DR eggNOG; KOG1601; Eukaryota.
DR HOGENOM; CLU_336200_0_0_1; -.
DR InParanoid; Q10280; -.
DR OMA; FMKINGV; -.
DR PhylomeDB; Q10280; -.
DR Reactome; R-SPO-9018519; Estrogen-dependent gene expression.
DR PRO; PR:Q10280; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0032153; C:cell division site; HDA:PomBase.
DR GO; GO:0005737; C:cytoplasm; IDA:PomBase.
DR GO; GO:0005829; C:cytosol; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; IDA:PomBase.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IDA:PomBase.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IPI:PomBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:PomBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:PomBase.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0010515; P:negative regulation of induction of conjugation with cellular fusion; IMP:PomBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:PomBase.
DR CDD; cd00202; ZnF_GATA; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR013860; AreA_GATA.
DR InterPro; IPR039355; Transcription_factor_GATA.
DR InterPro; IPR000679; Znf_GATA.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR PANTHER; PTHR10071; PTHR10071; 1.
DR Pfam; PF08550; DUF1752; 1.
DR Pfam; PF00320; GATA; 1.
DR PRINTS; PR00619; GATAZNFINGER.
DR SMART; SM00401; ZnF_GATA; 1.
DR PROSITE; PS00344; GATA_ZN_FINGER_1; 1.
DR PROSITE; PS50114; GATA_ZN_FINGER_2; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Metal-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..855
FT /note="Transcription factor gaf1"
FT /id="PRO_0000083475"
FT ZN_FING 635..659
FT /note="GATA-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00094"
FT REGION 72..126
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 149..184
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 229..287
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 412..483
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 602..643
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 680..768
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 72..116
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 149..166
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 229..255
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 412..480
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 624..643
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 687..716
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 730..768
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 150
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 727
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 729
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
SQ SEQUENCE 855 AA; 91777 MW; 3D932F83D2DE6774 CRC64;
MDLKFSNFFK EAEHPALVKK FDKSTTDESS SKEDYSTMSD LWKMFSKAKS ELSNGRRVEN
LTWRLMSINL QKNLTPNGDS NTLTPDTFSD PTAPSSAQSV PPTSSAETTA DNSDTEMKLN
PIPAYSVPAD TTGSSLMEFN YIQRRVRKTS FDESTAKSKK RSIADSHFPD PNAMQRPHDL
ESQPFSYPKI HASNSFNFVK RDIDSSNFSN LDASALPISP PSDFFSVHSH NLPNAPPSIP
ANSNNSASPN QRIKASPKHA DTDVLGLDFD MTPSEPSSFP ENGGFPSFVD ANTHEQTLFP
SSATNSFSFE HGSAGFPIPG SVPSTSYHAN TASEDGFSSS YNSQGLFGIS SPLSSGVTPN
QSFFPDVSGN NIFDVSRNNH EVSSPLIQSP GSYVSMPSIN MVSSLPISAP VPNSNSQFPR
RPNTFRTNSS KSVGQGSSGV DSNQENAESF NPSISSHNSA EWASGETTGH SSNSPLPGSD
MFSPQFMRVG TAMGVAPVRS NSSNNFGQNF FHQTSPQFSA VPHRKVSAQD TNLMGSSPGM
YNHMPYLNRA TSANSITSPG VLPEGMAASL KKRTTNTAAT PQAALPTTLD TKKDRSVSFN
INKNAEKPTV SNAAEDKKGD ANTRRANATN PTPTCTNCQT RTTPLWRRSP DGQPLCNACG
LFMKINGVVR PLSLKTDVIK KRNRGVGTSA TPKQSGGRKG STRKSSSKSS SAKSTAADMK
PKADSKSISP GFVGGNQSLS SERIPLDPSM RSPLQQQSSE NESKSQSMLS ANNLNAGVND
FGLGFSEGLG SAHLDSNDSS MVQGKNDFAP VVDSPLFDAF DTDLGMSSVA ESHTMNMDPS
DLSRVSKSWD WYSVM
