GAI1_VITVI
ID GAI1_VITVI Reviewed; 590 AA.
AC Q8S4W7; Q84MI8;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=DELLA protein GAI1;
DE AltName: Full=Gibberellic acid-insensitive mutant protein 1;
DE AltName: Full=VvGAI1;
GN Name=GAI1;
OS Vitis vinifera (Grape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; Vitales; Vitaceae; Viteae; Vitis.
OX NCBI_TaxID=29760;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT HIS-38.
RC STRAIN=cv. Pinot Meunier;
RX PubMed=11976683; DOI=10.1038/416847a;
RA Boss P.K., Thomas M.R.;
RT "Association of dwarfism and floral induction with a grape 'green
RT revolution' mutation.";
RL Nature 416:847-850(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 294-440.
RC STRAIN=cv. Riesling;
RA Joly D., Perrin M., Gertz C., Masson J.E.;
RL Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Probable transcriptional regulator that acts as a repressor
CC of the gibberellin (GA) signaling pathway. Probably acts by
CC participating in large multiprotein complexes that repress
CC transcription of GA-inducible genes. Upon GA application, it is
CC degraded by the proteasome, allowing the GA signaling pathway.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- DOMAIN: The DELLA motif is required for its GA-induced degradation.
CC {ECO:0000250}.
CC -!- PTM: Phosphorylated. {ECO:0000250}.
CC -!- PTM: Ubiquitinated. Upon GA application it is ubiquitinated, leading to
CC its subsequent degradation (By similarity). {ECO:0000250}.
CC -!- POLYMORPHISM: In cv. Pinot Meunier, 1 of the 3 cultivars used for the
CC sparkling wine Champagne, plants are genetically indistinguishable from
CC Pinot noir in most cells, but their outer layer, the 'L1' epidermal
CC cell layer, is different: cv. Pinot Meunier has a furry surface on its
CC leaves whereas Pinot noir does not. This is due to the Ser-387 variant
CC that is present only in cells of the L1 layer that causes this
CC difference, demonstrating that GA inhibits flowering in grapewine.
CC -!- SIMILARITY: Belongs to the GRAS family. DELLA subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=All things dwarfed and
CC beautiful - Issue 70 of May 2006;
CC URL="https://web.expasy.org/spotlight/back_issues/070";
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DR EMBL; AF378125; AAM19210.1; -; Genomic_DNA.
DR EMBL; AY256862; AAP20096.1; -; mRNA.
DR RefSeq; XP_002284648.1; XM_002284612.4.
DR AlphaFoldDB; Q8S4W7; -.
DR SMR; Q8S4W7; -.
DR STRING; 29760.VIT_01s0011g05260.t01; -.
DR EnsemblPlants; Vitvi01g00446_t001; Vitvi01g00446_P001; Vitvi01g00446.
DR GeneID; 100232937; -.
DR Gramene; Vitvi01g00446_t001; Vitvi01g00446_P001; Vitvi01g00446.
DR KEGG; vvi:100232937; -.
DR eggNOG; ENOG502QPMG; Eukaryota.
DR HOGENOM; CLU_011924_4_0_1; -.
DR OrthoDB; 559310at2759; -.
DR ExpressionAtlas; Q8S4W7; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR GO; GO:0009740; P:gibberellic acid mediated signaling pathway; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.1290; -; 1.
DR InterPro; IPR038088; DELLA_N_sf.
DR InterPro; IPR030006; TF_DELLA.
DR InterPro; IPR021914; TF_DELLA_N.
DR InterPro; IPR005202; TF_GRAS.
DR PANTHER; PTHR31636; PTHR31636; 1.
DR PANTHER; PTHR31636:SF247; PTHR31636:SF247; 1.
DR Pfam; PF12041; DELLA; 1.
DR Pfam; PF03514; GRAS; 1.
DR PROSITE; PS50985; GRAS; 1.
PE 2: Evidence at transcript level;
KW Gibberellin signaling pathway; Nucleus; Phosphoprotein; Repressor;
KW Transcription; Transcription regulation; Ubl conjugation.
FT CHAIN 1..590
FT /note="DELLA protein GAI1"
FT /id="PRO_0000132248"
FT DOMAIN 205..575
FT /note="GRAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 153..182
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 212..266
FT /note="Leucine repeat I (LRI)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 284..349
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 363..395
FT /note="Leucine repeat II (LRII)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 405..496
FT /note="PFYRE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 499..575
FT /note="SAW"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 35..39
FT /note="DELLA motif"
FT MOTIF 315..319
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 413..417
FT /note="LXXLL motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT VARIANT 38
FT /note="L -> H (in Pinot Meunier L1 layer; induces the
FT formation of influorescence instead of grapewine tendrils)"
FT /evidence="ECO:0000269|PubMed:11976683"
FT CONFLICT 387
FT /note="N -> S (in Ref. 2; AAP20096)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 590 AA; 64866 MW; 0D9CF844C81C0001 CRC64;
MKREYHHPHH PTCSTSPTGK GKMWDADPQQ DAGMDELLAV LGYNVKASDM AEVAQKLEQL
EEVIVNAQED GLSHLASETV HYNPSDLSNW LGSMLSEFNP TPNCALDNPF LPPISPLDYT
NCSTQPKQEP SIFDSPSLDY DLKAIPGKAL YSHIEQPPQQ PPAPPLYQRD NKRLKPTTSA
TANSVSSVIG GWGVPTESAR PVVLVDSQET GIRLVHTLMA CAEAVQQENL KLAEALVKQI
GFLAVSQAGA MRKVATYFAE GLARRIYRLY PDKPLDSSFS DILQMHFYET CPYLKFAHFT
ANQAILEAFE GKKRVHVIDF SMKQGMQWPA LMQALALRPG GPPSFRLTGI GPPSTDNTDH
LHEVGWKLAQ LAETIHVEFE YRGFVANSLA DLDASMLELR DGESVAVNSV FELHSLLARP
GGIERVLSAV KDMKPDIVTI VEQEANHNGP VFLDRFTESL HYYSTLFDSL EGCGVSPVNT
QDKLMSEVYL GQQICNVVAC EGPERVERHE TLAQWRARLG SAGFDPVNLG SNAFKQASML
LALFAGGDGY RVEENNGCLM LGWHTRPLIA TSAWQLANKP ALPSSTPASN
