ID   GAIPB_CUCMA             Reviewed;         587 AA.
AC   Q6EI05;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 56.
DE   RecName: Full=DELLA protein GAIP-B;
DE   AltName: Full=CmGAIP-B;
DE            Short=GAIP-B;
DE   AltName: Full=Gibberellic acid-insensitive phloem protein B;
GN   Name=GAIPB;
OS   Cucurbita maxima (Pumpkin) (Winter squash).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Cucurbiteae; Cucurbita.
OX   NCBI_TaxID=3661;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=15773853; DOI=10.1111/j.1365-313x.2005.02351.x;
RA   Haywood V., Yu T.-S., Huang N.-C., Lucas W.J.;
RT   "Phloem long-distance trafficking of GIBBERELLIC ACID-INSENSITIVE RNA
RT   regulates leaf development.";
RL   Plant J. 42:49-68(2005).
CC   -!- FUNCTION: Probable transcriptional regulator that acts as a repressor
CC       of the gibberellin (GA) signaling pathway. Probably acts by
CC       participating in large multiprotein complexes that represses
CC       transcription of GA-inducible genes. Upon GA application, it is
CC       degraded by the proteasome, allowing the GA signaling pathway (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- DOMAIN: The DELLA motif is required for its GA-induced degradation.
CC       {ECO:0000250}.
CC   -!- PTM: Phosphorylated. {ECO:0000250}.
CC   -!- PTM: Ubiquitinated. Upon GA application it is ubiquitinated, leading to
CC       its subsequent degradation (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the GRAS family. DELLA subfamily. {ECO:0000305}.
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DR   EMBL; AY326307; AAQ96165.1; -; mRNA.
DR   AlphaFoldDB; Q6EI05; -.
DR   SMR; Q6EI05; -.
DR   Proteomes; UP000504608; Unplaced.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0009740; P:gibberellic acid mediated signaling pathway; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.1290; -; 1.
DR   InterPro; IPR038088; DELLA_N_sf.
DR   InterPro; IPR030006; TF_DELLA.
DR   InterPro; IPR021914; TF_DELLA_N.
DR   InterPro; IPR005202; TF_GRAS.
DR   PANTHER; PTHR31636; PTHR31636; 1.
DR   PANTHER; PTHR31636:SF247; PTHR31636:SF247; 1.
DR   Pfam; PF12041; DELLA; 1.
DR   Pfam; PF03514; GRAS; 1.
DR   PROSITE; PS50985; GRAS; 1.
PE   2: Evidence at transcript level;
KW   Gibberellin signaling pathway; Nucleus; Phosphoprotein; Reference proteome;
KW   Repressor; Transcription; Transcription regulation; Ubl conjugation.
FT   CHAIN           1..587
FT                   /note="DELLA protein GAIP-B"
FT                   /id="PRO_0000132242"
FT   DOMAIN          209..577
FT                   /note="GRAS"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          1..23
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          216..270
FT                   /note="Leucine repeat I (LRI)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          288..353
FT                   /note="VHIID"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          367..399
FT                   /note="Leucine repeat II (LRII)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          411..498
FT                   /note="PFYRE"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          501..577
FT                   /note="SAW"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   MOTIF           46..50
FT                   /note="DELLA motif"
FT   MOTIF           319..323
FT                   /note="VHIID"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   MOTIF           419..423
FT                   /note="LXXLL motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
SQ   SEQUENCE   587 AA;  65189 MW;  7BC80F6546F3D81D CRC64;
     MKREHHHLHP RPDPPSMAAA PNGDTYLNTG KAKLWEEDAQ LDGGMDELLA VLGYKVKSSD
     MAEVAQKLEQ LEEAMCQVQD TGLSHLAFDT VHYNPSDLST WLESMITELH PPPSFPQPHP
     SQMNDSSFLA PAESSTITSI DYDPQRQTSS LIFEESSSSD YDLKAITSSA IYSPRENKRL
     KPSSESDSDL FSTSAIGASN SATRPIVLVD SQENGIQLVH ALMACAEAVQ QNNLNLAEAL
     EKRIGYLAVS QAGAMRKVAT FFAEALARRI YRVCPENPLD HSMSDMLQLH FYESSPYLKF
     AHFTANQAIL EAFEGKKRVH VIDFSMNQGM QWPALLQALA LRPSGPPAFR LTGIGPPAPD
     NSDYLQDVGW KLAKLVETIN VEFEYRGFVA NSLADLDASM LELRPSEVES VVVNSVFELH
     KLLARPGAIE KVMSVVKQMK PEIMTVVEQE ANHNGPVFMD RFTESLHYYS TLFDSLESSP
     NNQDKMMSEM YLGKQICNVV ACEGSDRVEW HETLTQWRTR LCSSGFEPIH LGSNAFKQAS
     MLLALFGSGE GYRVEENNGS LTLGWHTRPL IVTSAWKLGN NSVVVTH