ID   GAIP_CUCMA              Reviewed;         579 AA.
AC   Q6EI06;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 53.
DE   RecName: Full=DELLA protein GAIP;
DE   AltName: Full=CmGAIP;
DE            Short=GAIP;
DE   AltName: Full=Gibberellic acid-insensitive phloem protein;
GN   Name=GAIP;
OS   Cucurbita maxima (Pumpkin) (Winter squash).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Cucurbiteae; Cucurbita.
OX   NCBI_TaxID=3661;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=15773853; DOI=10.1111/j.1365-313x.2005.02351.x;
RA   Haywood V., Yu T.-S., Huang N.-C., Lucas W.J.;
RT   "Phloem long-distance trafficking of GIBBERELLIC ACID-INSENSITIVE RNA
RT   regulates leaf development.";
RL   Plant J. 42:49-68(2005).
CC   -!- FUNCTION: Probable transcriptional regulator that acts as a repressor
CC       of the gibberellin (GA) signaling pathway. Probably acts by
CC       participating in large multiprotein complexes that represses
CC       transcription of GA-inducible genes. Upon GA application, it is
CC       degraded by the proteasome, allowing the GA signaling pathway (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- DOMAIN: The DELLA motif is required for its GA-induced degradation.
CC       {ECO:0000250}.
CC   -!- PTM: Phosphorylated. {ECO:0000250}.
CC   -!- PTM: Ubiquitinated. Upon GA application it is ubiquitinated, leading to
CC       its subsequent degradation (By similarity). {ECO:0000250}.
CC   -!- MISCELLANEOUS: Its mRNA transcript may be transported over long
CC       distance in the phloem stream. Such trafficking may regulate leaf
CC       development.
CC   -!- SIMILARITY: Belongs to the GRAS family. DELLA subfamily. {ECO:0000305}.
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DR   EMBL; AY326306; AAQ96164.1; -; mRNA.
DR   AlphaFoldDB; Q6EI06; -.
DR   SMR; Q6EI06; -.
DR   Proteomes; UP000504608; Unplaced.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0009740; P:gibberellic acid mediated signaling pathway; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.1290; -; 1.
DR   InterPro; IPR038088; DELLA_N_sf.
DR   InterPro; IPR030006; TF_DELLA.
DR   InterPro; IPR021914; TF_DELLA_N.
DR   InterPro; IPR005202; TF_GRAS.
DR   PANTHER; PTHR31636; PTHR31636; 1.
DR   PANTHER; PTHR31636:SF247; PTHR31636:SF247; 1.
DR   Pfam; PF12041; DELLA; 1.
DR   Pfam; PF03514; GRAS; 1.
DR   PROSITE; PS50985; GRAS; 1.
PE   2: Evidence at transcript level;
KW   Gibberellin signaling pathway; Nucleus; Phosphoprotein; Reference proteome;
KW   Repressor; Transcription; Transcription regulation; Ubl conjugation.
FT   CHAIN           1..579
FT                   /note="DELLA protein GAIP"
FT                   /id="PRO_0000132241"
FT   DOMAIN          202..570
FT                   /note="GRAS"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          209..263
FT                   /note="Leucine repeat I (LRI)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          281..346
FT                   /note="VHIID"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          360..392
FT                   /note="Leucine repeat II (LRII)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          404..491
FT                   /note="PFYRE"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          494..570
FT                   /note="SAW"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   MOTIF           46..50
FT                   /note="DELLA motif"
FT   MOTIF           312..316
FT                   /note="VHIID"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   MOTIF           412..416
FT                   /note="LXXLL motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
SQ   SEQUENCE   579 AA;  64442 MW;  55B78C49199C9AD7 CRC64;
     MKREHHYLHP RPEPPSVATG SNRESYLNTG KAKLWEEEVQ LDGGMDELLA VLGYKVKSSD
     MAEVAQKLEQ LEEAMCQVQD TGLSHLAFDT VHYNPSDLST WVESMLTELH PPPTSHLDDS
     SFLAPAESST IANVDYEPQL QTSSRIFEES SSSDYDLKAI TDSAIYSPRE SKRLKASESD
     TDVFSTSAIG ASNFATRPVV LVDSQENGIQ LVHALMVCAE AVQQNNLNLA EALVKRIDYL
     AVSQAGAMRK VATFFAEALA RRIYRLCPEN PLDRSVLDML QMHFYESCPY LKFAHFTANQ
     AILEAFEGKK RVHVIDFSMN QGIQWPALIQ ALALRPSGPP TFRLTGIGPP APDNSDYLQD
     VGWKLVKFAE TLHVEFEYRG FVANSLADLD ASMLELRPSE VESVVVNSVF ELHQLLARPG
     AIEKVLSVVK QMKPEIVTVV EQEANHNGPV FVERFTESLH YYSTLFDSLE CSPNSQDKMM
     SEMYLGKQIC NVVACEGADR VERHETLTQW RTRLSSAGFD PIHLGSNAFK QASILLALFG
     SGEGYRVEEN EGSLMLGWHT RPLIATSAWK PGNNPVVAH