GAI_GOSHI
ID GAI_GOSHI Reviewed; 537 AA.
AC Q84TQ7; Q4ZIN2;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 61.
DE RecName: Full=DELLA protein GAI;
DE AltName: Full=GhGAI;
DE AltName: Full=Gibberellic acid-insensitive mutant protein;
GN Name=GAI;
OS Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=3635;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Fiber;
RA Luo M., Xiao Y., Hou L., Luo X., Li D., Zhang Z., Pei Y.;
RT "Molecular cloning and expression analysis of a gene (GhGAI) from cotton
RT (Gossypium hirsutum L.).";
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Liao W.B., Cui B.M., Wei H.B., Yu X.L., Li J.H., Peng M.;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Probable transcriptional regulator that acts as a repressor
CC of the gibberellin (GA) signaling pathway. Probably acts by
CC participating in large multiprotein complexes that represses
CC transcription of GA-inducible genes. Upon GA application, it is
CC degraded by the proteasome, allowing the GA signaling pathway (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- DOMAIN: The DELLA motif is required for its GA-induced degradation.
CC {ECO:0000250}.
CC -!- PTM: Phosphorylated. {ECO:0000250}.
CC -!- PTM: Ubiquitinated. Upon GA application it is ubiquitinated, leading to
CC its subsequent degradation (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the GRAS family. DELLA subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAY28970.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AY208992; AAO62757.1; -; mRNA.
DR EMBL; DQ006269; AAY28970.1; ALT_FRAME; Genomic_DNA.
DR AlphaFoldDB; Q84TQ7; -.
DR SMR; Q84TQ7; -.
DR Proteomes; UP000189702; Genome assembly.
DR GO; GO:0005634; C:nucleus; IDA:AgBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR GO; GO:0009740; P:gibberellic acid mediated signaling pathway; IDA:AgBase.
DR GO; GO:0042538; P:hyperosmotic salinity response; IBA:GO_Central.
DR GO; GO:0009867; P:jasmonic acid mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0009938; P:negative regulation of gibberellic acid mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0010187; P:negative regulation of seed germination; IBA:GO_Central.
DR GO; GO:2000273; P:positive regulation of signaling receptor activity; IDA:AgBase.
DR GO; GO:2000377; P:regulation of reactive oxygen species metabolic process; IBA:GO_Central.
DR GO; GO:2000033; P:regulation of seed dormancy process; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:AgBase.
DR GO; GO:0009737; P:response to abscisic acid; IBA:GO_Central.
DR GO; GO:0009723; P:response to ethylene; IBA:GO_Central.
DR GO; GO:0009739; P:response to gibberellin; IDA:AgBase.
DR GO; GO:0009863; P:salicylic acid mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0090378; P:seed trichome elongation; IDA:AgBase.
DR Gene3D; 1.10.10.1290; -; 1.
DR InterPro; IPR038088; DELLA_N_sf.
DR InterPro; IPR030006; TF_DELLA.
DR InterPro; IPR021914; TF_DELLA_N.
DR InterPro; IPR005202; TF_GRAS.
DR PANTHER; PTHR31636; PTHR31636; 1.
DR PANTHER; PTHR31636:SF47; PTHR31636:SF47; 1.
DR Pfam; PF12041; DELLA; 1.
DR Pfam; PF03514; GRAS; 1.
DR PROSITE; PS50985; GRAS; 1.
PE 2: Evidence at transcript level;
KW Gibberellin signaling pathway; Nucleus; Phosphoprotein; Reference proteome;
KW Repressor; Transcription; Transcription regulation; Ubl conjugation.
FT CHAIN 1..537
FT /note="DELLA protein GAI"
FT /id="PRO_0000132243"
FT DOMAIN 162..533
FT /note="GRAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 131..157
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 169..223
FT /note="Leucine repeat I (LRI)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 241..306
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 320..352
FT /note="Leucine repeat II (LRII)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 364..454
FT /note="PFYRE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 457..533
FT /note="SAW"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 37..41
FT /note="DELLA motif"
FT MOTIF 272..276
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 372..376
FT /note="LXXLL motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT CONFLICT 125
FT /note="A -> T (in Ref. 2; AAY28970)"
FT /evidence="ECO:0000305"
FT CONFLICT 138
FT /note="I -> N (in Ref. 2; AAY28970)"
FT /evidence="ECO:0000305"
FT CONFLICT 167
FT /note="A -> T (in Ref. 2; AAY28970)"
FT /evidence="ECO:0000305"
FT CONFLICT 242..243
FT /note="IP -> MH (in Ref. 2; AAY28970)"
FT /evidence="ECO:0000305"
FT CONFLICT 265
FT /note="A -> T (in Ref. 2; AAY28970)"
FT /evidence="ECO:0000305"
FT CONFLICT 270
FT /note="S -> N (in Ref. 2; AAY28970)"
FT /evidence="ECO:0000305"
FT CONFLICT 314
FT /note="N -> D (in Ref. 2; AAY28970)"
FT /evidence="ECO:0000305"
FT CONFLICT 327
FT /note="Q -> E (in Ref. 2; AAY28970)"
FT /evidence="ECO:0000305"
FT CONFLICT 339
FT /note="R -> P (in Ref. 2; AAY28970)"
FT /evidence="ECO:0000305"
FT CONFLICT 437
FT /note="A -> P (in Ref. 2; AAY28970)"
FT /evidence="ECO:0000305"
FT CONFLICT 482
FT /note="V -> F (in Ref. 2; AAY28970)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 537 AA; 58902 MW; B9E01DE4761A62E1 CRC64;
MKRDHQEISG SGSNPAESSS IKGKLWEEDP DAGGMDDELL AVLGYKVRSS DMADVAQKLE
MLEKVMGTAQ EDGISQLGDT VHFNPSDLSG WVQNLLIEFN GSTTTPDPNF NDDSEYDLRA
IPGVAAYPPV KSDPGLEITR KRAKTESSSS SSSTTTRPVV LIDSQEAGVR LVHTLMACAE
AVQQDNLKLA DALVKHIGLL ASSQTGAMRK VATYFAEALA RRIYRIFPPD SLDPSYNDKL
QIPFYETCPY LKFAHFTANQ AILEAFSMAS RVHVIDFGLK QGMQWPALMQ ALALRPGGPP
AFRLTGIGPP QPDNTDALQQ VGWKLAQLAE RIGIEFEFRG FVANSLADLE PEMLDIRPPE
IEVVAVNAVF ELHPLLARPG GIEKVVSSIK AMKPKIVTVV EQEANHNGPV FLDRFTEALH
YYSTLFDSLE GSGVAPASQD LAMSELYLGR QICNVVACEG MDRVERHEPL TQWRTRMETA
GVSPVHLGSN AYKQASMLLA LFASGDGYRV EENNGCLMLG WHTRPLIAHL GLATRWY
