GAI_SOLLC
ID GAI_SOLLC Reviewed; 588 AA.
AC Q7Y1B6;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=DELLA protein GAI;
DE AltName: Full=Gibberellic acid-insensitive mutant protein;
GN Name=GAI;
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=15347801; DOI=10.1104/pp.103.034876;
RA Bassel G.W., Zielinska E., Mullen R.T., Bewley J.D.;
RT "Down-regulation of della genes is not essential for germination of tomato,
RT soybean, and Arabidopsis seeds.";
RL Plant Physiol. 136:2782-2789(2004).
CC -!- FUNCTION: Probable transcriptional regulator that acts as a repressor
CC of the gibberellin (GA) signaling pathway. Probably acts by
CC participating in large multiprotein complexes that repress
CC transcription of GA-inducible genes. Upon GA application, it is
CC degraded by the proteasome, allowing the GA signaling pathway (By
CC similarity). Its degradation is not essential for germination.
CC {ECO:0000250, ECO:0000269|PubMed:15347801}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in both vegetative and reproductive
CC tissues. {ECO:0000269|PubMed:15347801}.
CC -!- DOMAIN: The DELLA motif is required for its GA-induced degradation.
CC {ECO:0000250}.
CC -!- PTM: Phosphorylated. {ECO:0000250}.
CC -!- PTM: Ubiquitinated. Upon GA application it is ubiquitinated, leading to
CC its subsequent degradation (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the GRAS family. DELLA subfamily. {ECO:0000305}.
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DR EMBL; AY269087; AAP22369.1; -; mRNA.
DR RefSeq; NP_001234365.1; NM_001247436.1.
DR AlphaFoldDB; Q7Y1B6; -.
DR SMR; Q7Y1B6; -.
DR STRING; 4081.Solyc11g011260.1.1; -.
DR PaxDb; Q7Y1B6; -.
DR PRIDE; Q7Y1B6; -.
DR EnsemblPlants; Solyc11g011260.1.1; Solyc11g011260.1.1.1; Solyc11g011260.1.
DR GeneID; 543881; -.
DR Gramene; Solyc11g011260.1.1; Solyc11g011260.1.1.1; Solyc11g011260.1.
DR KEGG; sly:543881; -.
DR eggNOG; ENOG502QPMG; Eukaryota.
DR HOGENOM; CLU_011924_4_0_1; -.
DR InParanoid; Q7Y1B6; -.
DR OMA; VQQENFA; -.
DR OrthoDB; 559310at2759; -.
DR PhylomeDB; Q7Y1B6; -.
DR Proteomes; UP000004994; Chromosome 11.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR GO; GO:0009740; P:gibberellic acid mediated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0042538; P:hyperosmotic salinity response; IBA:GO_Central.
DR GO; GO:0009867; P:jasmonic acid mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0009938; P:negative regulation of gibberellic acid mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0010187; P:negative regulation of seed germination; IBA:GO_Central.
DR GO; GO:2000377; P:regulation of reactive oxygen species metabolic process; IBA:GO_Central.
DR GO; GO:2000033; P:regulation of seed dormancy process; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0009737; P:response to abscisic acid; IBA:GO_Central.
DR GO; GO:0009723; P:response to ethylene; IBA:GO_Central.
DR GO; GO:0009863; P:salicylic acid mediated signaling pathway; IBA:GO_Central.
DR Gene3D; 1.10.10.1290; -; 1.
DR InterPro; IPR038088; DELLA_N_sf.
DR InterPro; IPR030006; TF_DELLA.
DR InterPro; IPR021914; TF_DELLA_N.
DR InterPro; IPR005202; TF_GRAS.
DR PANTHER; PTHR31636; PTHR31636; 1.
DR PANTHER; PTHR31636:SF47; PTHR31636:SF47; 1.
DR Pfam; PF12041; DELLA; 1.
DR Pfam; PF03514; GRAS; 1.
DR PROSITE; PS50985; GRAS; 1.
PE 2: Evidence at transcript level;
KW Gibberellin signaling pathway; Nucleus; Phosphoprotein; Reference proteome;
KW Repressor; Transcription; Transcription regulation; Ubl conjugation.
FT CHAIN 1..588
FT /note="DELLA protein GAI"
FT /id="PRO_0000132245"
FT DOMAIN 190..574
FT /note="GRAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 1..38
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 152..177
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 197..251
FT /note="Leucine repeat I (LRI)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 269..334
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 348..380
FT /note="Leucine repeat II (LRII)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 392..495
FT /note="PFYRE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 498..574
FT /note="SAW"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 42..46
FT /note="DELLA motif"
FT MOTIF 300..304
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 400..404
FT /note="LXXLL motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT COMPBIAS 1..16
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 158..177
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 588 AA; 64526 MW; 1EDADAF6BE100621 CRC64;
MKRDRDRDRE REKRAFSNGA VSSGKSKIWE EDEEEKPDAG MDELLAVLGY KVKSSDMADV
AQKLEQLEMA MGTTMEDGIT HLSTDTVHKN PSDMAGWVQS MLSSISTNFD MCNQENDVLV
SGCGSSSSII DFSQNHRTST ISDDDLRAIP GGAVFNSDSN KRHRSTTSSF STTSSSMVTD
SSATRPVVLV DSQETGVRLV HTLMACAEAV QQENLTLADQ LVRHIGILAV SQSGAMRKVA
TYFAEALARR IYKIYPQDSM ESSYTDVLQM HFYETCPYLK FAHFTANQAI LEAFTGCNKV
HVIDFSLKQG MQWPALMQAL ALRPGGPPAF RLTGIGPPQP DNTDALQQVG WKLAQLAETI
GVEFEFRGFV ANSLADLDAT ILDIRPSETE AVAINSVFEL HRLLSRPGAI EKVLNSIKQI
NPKIVTLVEQ EANHNAGVFI DRFNEALHYY STMFDSLESS GSSSSASPTG ILPQPPVNNQ
DLVMSEVYLG RQICNVVACE GSDRVERHET LNQWRVRMNS SGFDPVHLGS NAFKQASMLL
ALFAGGDGYR VEENDGCLML GWHTRPLIAT SAWKLLPDSG TGAGEVEL
