GAK1A_MOUSE
ID GAK1A_MOUSE Reviewed; 562 AA.
AC Q3UY90; Q3TYS0; Q3UTV8; Q8C7H6;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Golgi-associated kinase 1A {ECO:0000305};
DE AltName: Full=Protein FAM198A;
DE Flags: Precursor;
GN Name=Gask1a; Synonyms=Fam198a {ECO:0000312|MGI:MGI:3041196};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC STRAIN=C57BL/6J; TISSUE=Aorta, Inner ear, Liver, and Olfactory bulb;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP SUBCELLULAR LOCATION, PROTEOLYTIC CLEAVAGE, AND TISSUE SPECIFICITY.
RX PubMed=30188967; DOI=10.1093/abbs/gmy105;
RA Wei Z., Liu T., Lei J., Wu Y., Wang S., Liao K.;
RT "Fam198a, a member of secreted kinase, secrets through caveolae biogenesis
RT pathway.";
RL Acta Biochim. Biophys. Sin. 50:968-975(2018).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30188967}.
CC Endoplasmic reticulum {ECO:0000269|PubMed:30188967}. Golgi apparatus
CC {ECO:0000269|PubMed:30188967}. Membrane, caveola
CC {ECO:0000269|PubMed:30188967}. Note=Requires caveolae biogenesis to be
CC secreted from the endoplasmic reticulum going through the Golgi
CC apparatus where is post-translationally processed to the mature form.
CC {ECO:0000269|PubMed:30188967}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q3UY90-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3UY90-2; Sequence=VSP_027885;
CC Name=3;
CC IsoId=Q3UY90-3; Sequence=VSP_027886;
CC -!- TISSUE SPECIFICITY: Expressed in tracheal serous gland, heart, muscle,
CC fat, lung, ovary, liver, kidney, adrenal gland and brain (at protein
CC level). {ECO:0000269|PubMed:30188967}.
CC -!- PTM: Proteolytically cleaved. Cleaved at Arg-112 and Arg-424 leading to
CC a processed mature product of 35 kDa. The cleavage takes place in the
CC Golgi apparatus. {ECO:0000269|PubMed:30188967}.
CC -!- SIMILARITY: Belongs to the GASK family. {ECO:0000305}.
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DR EMBL; AK050223; BAC34133.1; -; mRNA.
DR EMBL; AK134876; BAE22322.1; -; mRNA.
DR EMBL; AK139038; BAE23872.1; -; mRNA.
DR EMBL; AK158406; BAE34492.1; -; mRNA.
DR EMBL; BC113767; AAI13768.1; -; mRNA.
DR CCDS; CCDS52969.1; -. [Q3UY90-2]
DR RefSeq; NP_001186856.1; NM_001199927.1. [Q3UY90-3]
DR RefSeq; NP_808411.1; NM_177743.5. [Q3UY90-2]
DR RefSeq; XP_006512193.1; XM_006512130.1. [Q3UY90-1]
DR RefSeq; XP_006512194.1; XM_006512131.2. [Q3UY90-2]
DR AlphaFoldDB; Q3UY90; -.
DR STRING; 10090.ENSMUSP00000040221; -.
DR PhosphoSitePlus; Q3UY90; -.
DR PaxDb; Q3UY90; -.
DR PRIDE; Q3UY90; -.
DR ProteomicsDB; 271830; -. [Q3UY90-1]
DR ProteomicsDB; 271831; -. [Q3UY90-2]
DR ProteomicsDB; 271832; -. [Q3UY90-3]
DR Antibodypedia; 48543; 5 antibodies from 5 providers.
DR DNASU; 245050; -.
DR Ensembl; ENSMUST00000043011; ENSMUSP00000040221; ENSMUSG00000038233. [Q3UY90-2]
DR Ensembl; ENSMUST00000213773; ENSMUSP00000151175; ENSMUSG00000038233. [Q3UY90-2]
DR Ensembl; ENSMUST00000214536; ENSMUSP00000150724; ENSMUSG00000038233. [Q3UY90-3]
DR Ensembl; ENSMUST00000215990; ENSMUSP00000149755; ENSMUSG00000038233. [Q3UY90-1]
DR GeneID; 245050; -.
DR KEGG; mmu:245050; -.
DR UCSC; uc009sei.2; mouse. [Q3UY90-1]
DR UCSC; uc012hcv.1; mouse. [Q3UY90-3]
DR CTD; 729085; -.
DR MGI; MGI:3041196; Gask1a.
DR VEuPathDB; HostDB:ENSMUSG00000038233; -.
DR eggNOG; ENOG502RYY5; Eukaryota.
DR GeneTree; ENSGT00420000029769; -.
DR HOGENOM; CLU_033542_1_0_1; -.
DR InParanoid; Q3UY90; -.
DR OrthoDB; 451767at2759; -.
DR PhylomeDB; Q3UY90; -.
DR TreeFam; TF330994; -.
DR BioGRID-ORCS; 245050; 1 hit in 73 CRISPR screens.
DR PRO; PR:Q3UY90; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q3UY90; protein.
DR Bgee; ENSMUSG00000038233; Expressed in nasal concha cartilage and 50 other tissues.
DR GO; GO:0005901; C:caveola; IEA:UniProtKB-SubCell.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:UniProtKB.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR InterPro; IPR029207; FAM198.
DR PANTHER; PTHR15905; PTHR15905; 1.
DR Pfam; PF15051; FAM198; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Endoplasmic reticulum; Golgi apparatus; Membrane;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..562
FT /note="Golgi-associated kinase 1A"
FT /id="PRO_0000301949"
FT PROPEP 30..111
FT /note="Removed in mature form"
FT /evidence="ECO:0000250|UniProtKB:Q9UFP1"
FT /id="PRO_0000446053"
FT PROPEP 424..562
FT /note="Removed in mature form"
FT /evidence="ECO:0000250|UniProtKB:Q9UFP1"
FT /id="PRO_0000446054"
FT REGION 179..229
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 201..225
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 111..112
FT /note="Cleavage"
FT /evidence="ECO:0000250|UniProtKB:Q9UFP1"
FT SITE 423..424
FT /note="Cleavage"
FT /evidence="ECO:0000250|UniProtKB:Q9UFP1"
FT VAR_SEQ 1..16
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_027885"
FT VAR_SEQ 494..562
FT /note="FPESAVKVLASGCLQNLLFKSLQMDQVFWESQGGAAGLKHVLETLESRGQIL
FT LRHIKKHNLTLFRDKDP -> IYHVCEGDYGSQKRTADSLELELLEWLCATAYRC (in
FT isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_027886"
FT CONFLICT 425
FT /note="Y -> H (in Ref. 1; BAE34492)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 562 AA; 62298 MW; F2A9D5F81F8E4A7E CRC64;
MVSWIWRRLR GKKRSVMAFC LLMVLSAVAV IHFPPGHPAS TPGLNPMEPR GEVGASDPRI
QQTLNSSLRQ PARNLGHWTG QALPRNPILV CAKKQSRRRQ VDRSRHPLSV RRDAILSAQD
RELLLEGEVR DAGGAALGQP GHNGLVQETQ SKTVTMVVPL TERSHESFQA QRDTAAASFR
PWPADGRDPL PGAKNGVLTG GKAGSATSGS EAPWWSSSAE DLQKSPWCGT ETPGLAGTAA
WGQVPPWFME HDAQTLRLLV HGKVVGKARV PAHGQVLQVG LSAGDALQDI SPLRLSQFCS
QGLCGLIKRS KDLHEVLSFH LDRVLGLQRS LPAVARSFHS PLLPYRYTDG SVRPIIWWAP
DVQHLRDPDE DQNSLALGWL QYQALLARGC KWPGQIPCLG IHHAEWARLA LFDFLLQVHD
RLDRYCCGFE PEPSDPCVEE GLREKCRNPE ELRLVHILVR SSDPSRLVYI DNAGNLQHPE
DKLNFRLLEG IDGFPESAVK VLASGCLQNL LFKSLQMDQV FWESQGGAAG LKHVLETLES
RGQILLRHIK KHNLTLFRDK DP
