ID   GAL10_CANMA             Reviewed;         153 AA.
AC   P56600;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-1998, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Bifunctional protein GAL10;
DE   Includes:
DE     RecName: Full=UDP-glucose 4-epimerase;
DE              EC=5.1.3.2;
DE     AltName: Full=Galactowaldenase;
DE   Includes:
DE     RecName: Full=Aldose 1-epimerase;
DE              EC=5.1.3.3;
DE     AltName: Full=Galactose mutarotase;
DE   Flags: Fragment;
GN   Name=GAL10;
OS   Candida maltosa (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX   NCBI_TaxID=5479;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 28140 / CBS 5611 / IAM 12247 / JCM 1504 / NBRC 1977;
RX   PubMed=9046083;
RX   DOI=10.1002/(sici)1097-0061(199701)13:1<21::aid-yea58>3.0.co;2-l;
RA   Park S.M., Ohkuma M., Masuda Y., Ohta A., Takagi M.;
RT   "Galactose-inducible expression systems in Candida maltosa using promoters
RT   of newly-isolated GAL1 and GAL10 genes.";
RL   Yeast 13:21-29(1997).
CC   -!- FUNCTION: Mutarotase converts alpha-aldose to the beta-anomer. It is
CC       active on D-glucose, L-arabinose, D-xylose, D-galactose, maltose and
CC       lactose (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=UDP-alpha-D-glucose = UDP-alpha-D-galactose;
CC         Xref=Rhea:RHEA:22168, ChEBI:CHEBI:58885, ChEBI:CHEBI:66914;
CC         EC=5.1.3.2;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-D-glucose = beta-D-glucose; Xref=Rhea:RHEA:10264,
CC         ChEBI:CHEBI:15903, ChEBI:CHEBI:17925; EC=5.1.3.3;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10126};
CC   -!- COFACTOR:
CC       Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC   -!- PATHWAY: Carbohydrate metabolism; galactose metabolism.
CC   -!- PATHWAY: Carbohydrate metabolism; hexose metabolism.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the NAD(P)-dependent
CC       epimerase/dehydratase family. {ECO:0000305}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the aldose epimerase
CC       family. {ECO:0000305}.
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DR   EMBL; D29759; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; P56600; -.
DR   SMR; P56600; -.
DR   UniPathway; UPA00214; -.
DR   UniPathway; UPA00242; -.
DR   GO; GO:0004034; F:aldose 1-epimerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003978; F:UDP-glucose 4-epimerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006012; P:galactose metabolic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF16363; GDP_Man_Dehyd; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Galactose metabolism; Isomerase;
KW   Multifunctional enzyme; NAD.
FT   CHAIN           1..>153
FT                   /note="Bifunctional protein GAL10"
FT                   /id="PRO_0000197438"
FT   REGION          1..>153
FT                   /note="Galactowaldenase"
FT   NON_TER         153
SQ   SEQUENCE   153 AA;  16752 MW;  A069AC3040BC978D CRC64;
     MSDDIFLVTG GAGYIGSHTV IELINHGIKV VIADNLSNSS YDAVARIEFI VKQHVSHFIK
     VDIRNEKELN QVFSNHKISG VIHFAALKAV GESTKIPLEY YDNNVSGTIA LLNVCKSNNV
     KTIVFSSSAT VYGDVTRFGD NSMIPIPEHC PME