GAL10_SCHPO
ID GAL10_SCHPO Reviewed; 713 AA.
AC Q9HDU3;
DT 11-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Bifunctional protein gal10;
DE Includes:
DE RecName: Full=UDP-glucose 4-epimerase;
DE EC=5.1.3.2;
DE AltName: Full=Galactowaldenase;
DE Includes:
DE RecName: Full=Aldose 1-epimerase;
DE EC=5.1.3.3;
DE AltName: Full=Galactose mutarotase;
GN Name=gal10; ORFNames=SPBPB2B2.12c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
CC -!- FUNCTION: Mutarotase converts alpha-aldose to the beta-anomer. It is
CC active on D-glucose, L-arabinose, D-xylose, D-galactose, maltose and
CC lactose (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=UDP-alpha-D-glucose = UDP-alpha-D-galactose;
CC Xref=Rhea:RHEA:22168, ChEBI:CHEBI:58885, ChEBI:CHEBI:66914;
CC EC=5.1.3.2;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-D-glucose = beta-D-glucose; Xref=Rhea:RHEA:10264,
CC ChEBI:CHEBI:15903, ChEBI:CHEBI:17925; EC=5.1.3.3;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10126};
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC -!- PATHWAY: Carbohydrate metabolism; galactose metabolism.
CC -!- PATHWAY: Carbohydrate metabolism; hexose metabolism.
CC -!- SIMILARITY: In the N-terminal section; belongs to the NAD(P)-dependent
CC epimerase/dehydratase family. {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the aldose epimerase
CC family. {ECO:0000305}.
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DR EMBL; CU329671; CAC21414.1; -; Genomic_DNA.
DR RefSeq; NP_596858.1; NM_001023881.2.
DR AlphaFoldDB; Q9HDU3; -.
DR SMR; Q9HDU3; -.
DR BioGRID; 277919; 6.
DR STRING; 4896.SPBPB2B2.12c.1; -.
DR iPTMnet; Q9HDU3; -.
DR MaxQB; Q9HDU3; -.
DR PaxDb; Q9HDU3; -.
DR EnsemblFungi; SPBPB2B2.12c.1; SPBPB2B2.12c.1:pep; SPBPB2B2.12c.
DR GeneID; 2541411; -.
DR KEGG; spo:SPBPB2B2.12c; -.
DR PomBase; SPBPB2B2.12c; gal10.
DR VEuPathDB; FungiDB:SPBPB2B2.12c; -.
DR eggNOG; KOG1371; Eukaryota.
DR HOGENOM; CLU_007383_22_1_1; -.
DR InParanoid; Q9HDU3; -.
DR OMA; KGLYREW; -.
DR PhylomeDB; Q9HDU3; -.
DR BRENDA; 5.1.3.2; 5613.
DR Reactome; R-SPO-70370; Galactose catabolism.
DR UniPathway; UPA00214; -.
DR UniPathway; UPA00242; -.
DR PRO; PR:Q9HDU3; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0004034; F:aldose 1-epimerase activity; IEA:UniProtKB-EC.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0003978; F:UDP-glucose 4-epimerase activity; IMP:PomBase.
DR GO; GO:0006012; P:galactose metabolic process; IMP:PomBase.
DR GO; GO:0042125; P:protein galactosylation; IMP:PomBase.
DR GO; GO:0052574; P:UDP-galactose biosynthetic process; IMP:PomBase.
DR CDD; cd05247; UDP_G4E_1_SDR_e; 1.
DR Gene3D; 2.70.98.10; -; 1.
DR InterPro; IPR018052; Ald1_epimerase_CS.
DR InterPro; IPR008183; Aldose_1/G6P_1-epimerase.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR014718; GH-type_carb-bd.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR005886; UDP_G4E.
DR Pfam; PF01263; Aldose_epim; 1.
DR Pfam; PF16363; GDP_Man_Dehyd; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
DR SUPFAM; SSF74650; SSF74650; 1.
DR TIGRFAMs; TIGR01179; galE; 1.
DR PROSITE; PS00545; ALDOSE_1_EPIMERASE; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Galactose metabolism; Isomerase;
KW Multifunctional enzyme; NAD; Reference proteome.
FT CHAIN 1..713
FT /note="Bifunctional protein gal10"
FT /id="PRO_0000197441"
FT REGION 1..350
FT /note="Galactowaldenase"
FT REGION 351..713
FT /note="Mutarotase"
FT ACT_SITE 532
FT /note="For mutarotase activity"
FT /evidence="ECO:0000255"
FT BINDING 7..38
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000255"
SQ SEQUENCE 713 AA; 80666 MW; 51C89DA0843A8556 CRC64;
MAVQDEYILV TGGAGYIGSH TVIELINHGY KVIIVDNLCN SCYDAVARVE FIVRKSIKFF
KLDLRDKEGL AQIFDTFKIK GVIHFAALKA VGESMKLPLE YYDNNICGTI TLLNVMREHR
VKTVVFSSSA TVYGDATRFD NMIPIPESCP NDPTNPYGKT KYAIENIIKD LHTSDNTWRG
AILRYFNPIG AHPSGLLGED PLGIPNNLLP FLAQVAIGRR EKLLVFGDDY DSHDGTPIRD
YIHVVDLAKG HIAALNYLNK INNSEGMYRE WNLGTGKGSS VFDIYHAFCK EVGKDLPYEV
VGRRTGDVLN LTASPNRANS ELKWKAELSI TDACRDLWKW TIENPFGFQI DNYKWKLFNT
LGIMDYKNRL HTICFQDLEV SIANYGALVQ AVRYKGRNLV NGFNDFSRYK LKENPFFGAT
IGRFANRIAN GQFEVDGHLY TLCKNENNKT TLHGGNNGFD KQFFLGPIAR QYEDYNTLEF
ILVDKDGNNG FPSDLETLVK YTIKNNSLEI EYKSVIPEYS KLNVTAVNLT NHSYWNLASP
NKTIDGTIIK STTNVYLKVN SETSLPTGDI VEWQNDITKP TKLDPNISFD NCFIVDREAS
KFCLDTRKYS LKNIVEVIHP SVPVKLVVST TEPAFQLYTG DGNDICEFQS RSGFCVETGR
FINALNNEKW SKQVILRKGE VYGARSKFSL YAQDLEENKH FLDSASYNSG EYY
