ID   GAL1_CANMA              Reviewed;         214 AA.
AC   P56599;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-1998, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Galactokinase;
DE            EC=2.7.1.6;
DE   AltName: Full=Galactose kinase;
DE   Flags: Fragment;
GN   Name=GAL1;
OS   Candida maltosa (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX   NCBI_TaxID=5479;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 28140 / CBS 5611 / IAM 12247 / JCM 1504 / NBRC 1977;
RX   PubMed=9046083;
RX   DOI=10.1002/(sici)1097-0061(199701)13:1<21::aid-yea58>3.0.co;2-l;
RA   Park S.M., Ohkuma M., Masuda Y., Ohta A., Takagi M.;
RT   "Galactose-inducible expression systems in Candida maltosa using promoters
RT   of newly-isolated GAL1 and GAL10 genes.";
RL   Yeast 13:21-29(1997).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-D-galactose + ATP = ADP + alpha-D-galactose 1-phosphate
CC         + H(+); Xref=Rhea:RHEA:13553, ChEBI:CHEBI:15378, ChEBI:CHEBI:28061,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:58336, ChEBI:CHEBI:456216; EC=2.7.1.6;
CC   -!- PATHWAY: Carbohydrate metabolism; galactose metabolism.
CC   -!- SIMILARITY: Belongs to the GHMP kinase family. GalK subfamily.
CC       {ECO:0000305}.
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DR   EMBL; D29759; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; P56599; -.
DR   SMR; P56599; -.
DR   UniPathway; UPA00214; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004335; F:galactokinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006012; P:galactose metabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.230.10; -; 1.
DR   InterPro; IPR000705; Galactokinase.
DR   InterPro; IPR019741; Galactokinase_CS.
DR   InterPro; IPR019539; GalKase_N.
DR   InterPro; IPR006204; GHMP_kinase_N_dom.
DR   InterPro; IPR006203; GHMP_knse_ATP-bd_CS.
DR   InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR   InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR   Pfam; PF10509; GalKase_gal_bdg; 1.
DR   Pfam; PF00288; GHMP_kinases_N; 1.
DR   PRINTS; PR00473; GALCTOKINASE.
DR   SUPFAM; SSF54211; SSF54211; 1.
DR   PROSITE; PS00106; GALACTOKINASE; 1.
DR   PROSITE; PS00627; GHMP_KINASES_ATP; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Carbohydrate metabolism; Galactose metabolism; Kinase;
KW   Nucleotide-binding; Transferase.
FT   CHAIN           1..>214
FT                   /note="Galactokinase"
FT                   /id="PRO_0000184653"
FT   ACT_SITE        199
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         147..157
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   SITE            47
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250"
FT   NON_TER         214
SQ   SEQUENCE   214 AA;  23418 MW;  45C79C2D05E5342A CRC64;
     MSISTVDDLS FYSNVEPNQQ RFTEVVKTFQ TNFPGDDITF FARSPGRVNL IGDHIDYNFF
     PVLPMAIAND VIAAVNVNST NEIIITNTDS KDFLKEVIAL RNSQIDQEHH SWANYFKCAL
     IVAKQYLEER GVTSLKGMKL TFNGNVPTGG GLSSSAAFCV ASTLAIIRAN GITDLTKQDL
     TRITVVSEHY VGVNTGGMDQ CASVCGEPDK LLLI