GAL1_HAEIN
ID GAL1_HAEIN Reviewed; 384 AA.
AC P31767;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Galactokinase {ECO:0000255|HAMAP-Rule:MF_00246};
DE EC=2.7.1.6 {ECO:0000255|HAMAP-Rule:MF_00246};
DE AltName: Full=Galactose kinase {ECO:0000255|HAMAP-Rule:MF_00246};
GN Name=galK {ECO:0000255|HAMAP-Rule:MF_00246}; OrderedLocusNames=HI_0819;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=RM 7004 / Serotype B;
RX PubMed=1282642; DOI=10.1111/j.1365-2958.1992.tb01763.x;
RA Maskell D.J., Szabo M.J., Deadman M.E., Moxon E.R.;
RT "The gal locus from Haemophilus influenzae: cloning, sequencing and the use
RT of gal mutants to study lipopolysaccharide.";
RL Mol. Microbiol. 6:3051-3063(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
CC -!- FUNCTION: Catalyzes the transfer of the gamma-phosphate of ATP to D-
CC galactose to form alpha-D-galactose-1-phosphate (Gal-1-P).
CC {ECO:0000255|HAMAP-Rule:MF_00246}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-D-galactose + ATP = ADP + alpha-D-galactose 1-phosphate
CC + H(+); Xref=Rhea:RHEA:13553, ChEBI:CHEBI:15378, ChEBI:CHEBI:28061,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:58336, ChEBI:CHEBI:456216; EC=2.7.1.6;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00246};
CC -!- PATHWAY: Carbohydrate metabolism; galactose metabolism.
CC {ECO:0000255|HAMAP-Rule:MF_00246}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00246}.
CC -!- SIMILARITY: Belongs to the GHMP kinase family. GalK subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00246}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC22478.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X65934; CAA46731.1; -; Genomic_DNA.
DR EMBL; L42023; AAC22478.1; ALT_INIT; Genomic_DNA.
DR PIR; D64096; D64096.
DR RefSeq; NP_438979.2; NC_000907.1.
DR RefSeq; WP_010869061.1; NC_000907.1.
DR AlphaFoldDB; P31767; -.
DR SMR; P31767; -.
DR STRING; 71421.HI_0819; -.
DR EnsemblBacteria; AAC22478; AAC22478; HI_0819.
DR KEGG; hin:HI_0819; -.
DR PATRIC; fig|71421.8.peg.860; -.
DR eggNOG; COG0153; Bacteria.
DR HOGENOM; CLU_017814_2_1_6; -.
DR PhylomeDB; P31767; -.
DR BioCyc; HINF71421:G1GJ1-860-MON; -.
DR UniPathway; UPA00214; -.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004335; F:galactokinase activity; IBA:GO_Central.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006012; P:galactose metabolic process; IBA:GO_Central.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.30.70.890; -; 1.
DR HAMAP; MF_00246; Galactokinase; 1.
DR InterPro; IPR000705; Galactokinase.
DR InterPro; IPR022963; Galactokinase_bac.
DR InterPro; IPR019741; Galactokinase_CS.
DR InterPro; IPR019539; GalKase_N.
DR InterPro; IPR013750; GHMP_kinase_C_dom.
DR InterPro; IPR036554; GHMP_kinase_C_sf.
DR InterPro; IPR006204; GHMP_kinase_N_dom.
DR InterPro; IPR006203; GHMP_knse_ATP-bd_CS.
DR InterPro; IPR006206; Mevalonate/galactokinase.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR PANTHER; PTHR10457:SF6; PTHR10457:SF6; 1.
DR Pfam; PF10509; GalKase_gal_bdg; 1.
DR Pfam; PF08544; GHMP_kinases_C; 1.
DR Pfam; PF00288; GHMP_kinases_N; 1.
DR PIRSF; PIRSF000530; Galactokinase; 1.
DR PRINTS; PR00473; GALCTOKINASE.
DR SUPFAM; SSF54211; SSF54211; 1.
DR SUPFAM; SSF55060; SSF55060; 1.
DR TIGRFAMs; TIGR00131; gal_kin; 1.
DR PROSITE; PS00106; GALACTOKINASE; 1.
DR PROSITE; PS00627; GHMP_KINASES_ATP; 1.
PE 3: Inferred from homology;
KW ATP-binding; Carbohydrate metabolism; Cytoplasm; Galactose metabolism;
KW Kinase; Magnesium; Metal-binding; Nucleotide-binding; Reference proteome;
KW Transferase.
FT CHAIN 1..384
FT /note="Galactokinase"
FT /id="PRO_0000184612"
FT ACT_SITE 173
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00246"
FT BINDING 34..37
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00246"
FT BINDING 123..129
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00246"
FT BINDING 129
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00246"
FT BINDING 161
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00246"
FT BINDING 222
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00246"
FT SITE 28
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00246"
FT VARIANT 107
FT /note="D -> H (in strain: RM 7004)"
FT VARIANT 145
FT /note="D -> N (in strain: RM 7004)"
FT VARIANT 321
FT /note="A -> V (in strain: RM 7004)"
FT VARIANT 331
FT /note="A -> T (in strain: RM 7004)"
FT VARIANT 353
FT /note="S -> A (in strain: RM 7004)"
SQ SEQUENCE 384 AA; 42132 MW; B28D552832858D34 CRC64;
MTPIQNAQQI FNRQHKNLPE ITVYAPGRVN IIGEHTDYND GFVMPCAINF GTAVSGTKRD
DHIWNVYAAD LDETDEFSLN VEIPKSEHKW ANYVRGVVKF IQERYPDFQQ GANLVISGNV
PLSSGLSSSA ALEVAVGKFC QQLGDLPLSH TDIALNGQKA ENQFVGANCG NMDQLISALG
QENHLLMIDC RSLETTPTPV PQDVAVIIVN SNVPHDLVTG EYNTRRQQCE EAAKFFGVKA
LRDVSVEQFQ KREAELTALS PLAAKRARHV VTENQRVLDA VEALKNNDLT CLGKLMEASH
DSMRDDFEIT VPQIDYLVEL AQLVIGKSGG ARMTGGGFGG CIVALAPHDK VDSVRKIIAD
NYEKTTGLKE TFYVCTASQG VRVI
