GAL1_KLULA
ID GAL1_KLULA Reviewed; 503 AA.
AC P09608;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1992, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Galactokinase;
DE EC=2.7.1.6;
DE AltName: Full=Galactose kinase;
GN Name=GAL1; OrderedLocusNames=KLLA0F08393g;
OS Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX NCBI_TaxID=284590;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1922058; DOI=10.1128/mcb.11.11.5454-5461.1991;
RA Meyer J., Walker-Jonah A., Hollenberg C.P.;
RT "Galactokinase encoded by GAL1 is a bifunctional protein required for
RT induction of the GAL genes in Kluyveromyces lactis and is able to suppress
RT the gal3 phenotype in Saccharomyces cerevisiae.";
RL Mol. Cell. Biol. 11:5454-5461(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX PubMed=15229592; DOI=10.1038/nature02579;
RA Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA Weissenbach J., Wincker P., Souciet J.-L.;
RT "Genome evolution in yeasts.";
RL Nature 430:35-44(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-178.
RC STRAIN=CBS 2359 / IFO 1267 / NRRL Y-1140 / WM37;
RX PubMed=3419917; DOI=10.1093/nar/16.16.8192;
RA Webster T.D., Dickson R.C.;
RT "Nucleotide sequence of the galactose gene cluster of Kluyveromyces
RT lactis.";
RL Nucleic Acids Res. 16:8192-8194(1988).
CC -!- FUNCTION: Seems bifunctional with both a galactokinase activity and the
CC ability to induce the GAL genes.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-D-galactose + ATP = ADP + alpha-D-galactose 1-phosphate
CC + H(+); Xref=Rhea:RHEA:13553, ChEBI:CHEBI:15378, ChEBI:CHEBI:28061,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:58336, ChEBI:CHEBI:456216; EC=2.7.1.6;
CC -!- PATHWAY: Carbohydrate metabolism; galactose metabolism.
CC -!- INDUCTION: The GAL1 gene is a member of the family of galactose
CC inducible, glucose-repressible genes (which include GAL2, GAL3, GAL7,
CC GAL10, GAL80, and MELI).
CC -!- SIMILARITY: Belongs to the GHMP kinase family. GalK subfamily.
CC {ECO:0000305}.
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DR EMBL; M74508; AAA35256.1; -; Genomic_DNA.
DR EMBL; CR382126; CAG98169.1; -; Genomic_DNA.
DR EMBL; M74111; AAA35255.1; -; Genomic_DNA.
DR EMBL; X07039; CAA30089.1; ALT_TERM; Genomic_DNA.
DR PIR; A41684; A41684.
DR RefSeq; XP_455461.1; XM_455461.1.
DR AlphaFoldDB; P09608; -.
DR SMR; P09608; -.
DR STRING; 28985.XP_455461.1; -.
DR MoonProt; P09608; -.
DR EnsemblFungi; CAG98169; CAG98169; KLLA0_F08393g.
DR GeneID; 2894951; -.
DR KEGG; kla:KLLA0_F08393g; -.
DR eggNOG; KOG0631; Eukaryota.
DR HOGENOM; CLU_017814_6_2_1; -.
DR InParanoid; P09608; -.
DR OMA; PVCYNLR; -.
DR UniPathway; UPA00214; -.
DR Proteomes; UP000000598; Chromosome F.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004335; F:galactokinase activity; IEA:UniProtKB-EC.
DR GO; GO:0006012; P:galactose metabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.30.230.10; -; 1.
DR InterPro; IPR000705; Galactokinase.
DR InterPro; IPR019741; Galactokinase_CS.
DR InterPro; IPR019539; GalKase_N.
DR InterPro; IPR013750; GHMP_kinase_C_dom.
DR InterPro; IPR036554; GHMP_kinase_C_sf.
DR InterPro; IPR006204; GHMP_kinase_N_dom.
DR InterPro; IPR006203; GHMP_knse_ATP-bd_CS.
DR InterPro; IPR006206; Mevalonate/galactokinase.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR Pfam; PF10509; GalKase_gal_bdg; 1.
DR Pfam; PF08544; GHMP_kinases_C; 1.
DR Pfam; PF00288; GHMP_kinases_N; 1.
DR PIRSF; PIRSF000530; Galactokinase; 1.
DR PRINTS; PR00473; GALCTOKINASE.
DR SUPFAM; SSF54211; SSF54211; 1.
DR SUPFAM; SSF55060; SSF55060; 1.
DR TIGRFAMs; TIGR00131; gal_kin; 1.
DR PROSITE; PS00106; GALACTOKINASE; 1.
DR PROSITE; PS00627; GHMP_KINASES_ATP; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Carbohydrate metabolism; Galactose metabolism; Kinase;
KW Nucleotide-binding; Reference proteome; Transferase.
FT CHAIN 1..503
FT /note="Galactokinase"
FT /id="PRO_0000184655"
FT ACT_SITE 205
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 150..160
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT SITE 43
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000250"
FT CONFLICT 57
FT /note="S -> H (in Ref. 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 59
FT /note="Missing (in Ref. 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 63
FT /note="I -> S (in Ref. 3; CAA30089)"
FT /evidence="ECO:0000305"
FT CONFLICT 125
FT /note="A -> V (in Ref. 3; CAA30089)"
FT /evidence="ECO:0000305"
FT CONFLICT 170
FT /note="I -> T (in Ref. 3; CAA30089)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 503 AA; 55805 MW; 6A66AD8B9ABDBC0F CRC64;
MSVPIVPTDF AHAPKLEQLK NEFVECYDTD DSRKFFITRS PGRVNLIGEH IDYCQFSVLP
MAIENDLMLA CRLTSESENP SITLTNHDSN FAQRKFDLPL DGSLIEIDPS VSDWSNYFKC
GLLVAQQFLQ EKYNFKGPVH GMEIYVKGDI PSGGGLSSSA AFICAVSLAI IYSNVPAGTP
ILKDELTKTT AVAEHHVGVN NGGMDQAASI CGIEGHALYV EFKPELKATP FKFPEDLPIS
FLIANTLVVS NKAETGPVNY NLRVVEVTVA ANVLAQKFGV TLQTEGNLGK GTLRNFMDSY
YTKYDKSCRK PWDGEIQTGI ERLNKMLQLV EETLDPNGYT LDHAVELCGC ESISQFTELY
LTNFPVRFQR LKLFQRAKHV YSEALRVLKA LQLFQKGESN FFEEFGALMN ESQESCDKLY
ECSCPETDSI CEIALKNGSF GSRLTGAGWG GCTVHLCSTD TVDSVKSALT EQYYNLRFPE
LTAEELEDAI IISKPSLGSV LYE
