GAL1_SCHPO
ID GAL1_SCHPO Reviewed; 519 AA.
AC Q9HDU2;
DT 11-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Galactokinase;
DE EC=2.7.1.6;
DE AltName: Full=Galactose kinase;
GN Name=gal1; ORFNames=SPBPB2B2.13;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-D-galactose + ATP = ADP + alpha-D-galactose 1-phosphate
CC + H(+); Xref=Rhea:RHEA:13553, ChEBI:CHEBI:15378, ChEBI:CHEBI:28061,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:58336, ChEBI:CHEBI:456216; EC=2.7.1.6;
CC -!- PATHWAY: Carbohydrate metabolism; galactose metabolism.
CC -!- SIMILARITY: Belongs to the GHMP kinase family. GalK subfamily.
CC {ECO:0000305}.
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DR EMBL; CU329671; CAC21415.1; -; Genomic_DNA.
DR RefSeq; NP_596859.1; NM_001023882.2.
DR AlphaFoldDB; Q9HDU2; -.
DR SMR; Q9HDU2; -.
DR BioGRID; 277891; 36.
DR STRING; 4896.SPBPB2B2.13.1; -.
DR MaxQB; Q9HDU2; -.
DR PaxDb; Q9HDU2; -.
DR EnsemblFungi; SPBPB2B2.13.1; SPBPB2B2.13.1:pep; SPBPB2B2.13.
DR GeneID; 2541380; -.
DR KEGG; spo:SPBPB2B2.13; -.
DR PomBase; SPBPB2B2.13; gal1.
DR VEuPathDB; FungiDB:SPBPB2B2.13; -.
DR eggNOG; KOG0631; Eukaryota.
DR HOGENOM; CLU_017814_6_2_1; -.
DR InParanoid; Q9HDU2; -.
DR OMA; PVCYNLR; -.
DR PhylomeDB; Q9HDU2; -.
DR UniPathway; UPA00214; -.
DR PRO; PR:Q9HDU2; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005737; C:cytoplasm; HDA:PomBase.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004335; F:galactokinase activity; ISO:PomBase.
DR GO; GO:0006012; P:galactose metabolic process; IBA:GO_Central.
DR GO; GO:0052574; P:UDP-galactose biosynthetic process; IC:PomBase.
DR Gene3D; 3.30.230.10; -; 1.
DR InterPro; IPR000705; Galactokinase.
DR InterPro; IPR019741; Galactokinase_CS.
DR InterPro; IPR019539; GalKase_N.
DR InterPro; IPR013750; GHMP_kinase_C_dom.
DR InterPro; IPR036554; GHMP_kinase_C_sf.
DR InterPro; IPR006204; GHMP_kinase_N_dom.
DR InterPro; IPR006203; GHMP_knse_ATP-bd_CS.
DR InterPro; IPR006206; Mevalonate/galactokinase.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR Pfam; PF10509; GalKase_gal_bdg; 1.
DR Pfam; PF08544; GHMP_kinases_C; 1.
DR Pfam; PF00288; GHMP_kinases_N; 1.
DR PIRSF; PIRSF000530; Galactokinase; 1.
DR PRINTS; PR00473; GALCTOKINASE.
DR SUPFAM; SSF54211; SSF54211; 1.
DR SUPFAM; SSF55060; SSF55060; 1.
DR TIGRFAMs; TIGR00131; gal_kin; 1.
DR PROSITE; PS00106; GALACTOKINASE; 1.
DR PROSITE; PS00627; GHMP_KINASES_ATP; 1.
PE 3: Inferred from homology;
KW ATP-binding; Carbohydrate metabolism; Galactose metabolism; Kinase;
KW Nucleotide-binding; Reference proteome; Transferase.
FT CHAIN 1..519
FT /note="Galactokinase"
FT /id="PRO_0000184656"
FT ACT_SITE 209
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 157..167
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT SITE 47
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000250"
SQ SEQUENCE 519 AA; 58385 MW; 5EE9C129896F8C40 CRC64;
MATVPTYHDL SFYSNPKENK ARYAKLLNSF EQKYHCKPDF FSRSPGRVNI IGEHIDYNYF
SVLPMAIDVD VIVSVTTSDD AKVELNNTNP EFKEEILELP SDGAVIEINK THHTWGNYFR
CSMIVAHKYI LEKYPELVSG GKKPLKGLKL IFDGNVPTGG GLSSSAAFCV ASILAILKAN
GINTITKEDL VKISVVSEHY VGVNTGGMDQ CASIYGEQNK ALLVQFKPKL MATPFKMPVL
KPHDMVFLIS NTLVEANKQE TALTNYNLRV VEMAVASEFL AKKFNLELPK ESNLHTGTLR
GFMDEYYEKH LKQPHWDGSD IDMGVQRMQE MLRLTEIMFS EEQKVGFKTE ELAKELGLSV
EEFTKVFLTK IPVKYERMKI YQRTVHVYSD AMRVLQVLKL FHQHKDSDDP QKFMLAFGRL
LNDSQRSEDI YNNSSSPELR EVCKISLANG GYGARTTGAG WGGSAVHLTT HDKLAKLVEA
LTEQYYKKQF PKITQSELNA AVVVSKPAAG SCIVQLAEY
