GAL2A_DANRE
ID GAL2A_DANRE Reviewed; 363 AA.
AC F1R332;
DT 26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2013, sequence version 2.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=Galanin receptor 2a {ECO:0000303|PubMed:24517231};
GN Name=galr2a {ECO:0000303|PubMed:24517231,
GN ECO:0000312|ZFIN:ZDB-GENE-130603-37};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000312|Proteomes:UP000000437};
RN [1] {ECO:0000312|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen {ECO:0000312|Proteomes:UP000000437};
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2] {ECO:0000305}
RP FUNCTION.
RX PubMed=24517231; DOI=10.1210/en.2013-2106;
RA Kim D.K., Yun S., Son G.H., Hwang J.I., Park C.R., Kim J.I., Kim K.,
RA Vaudry H., Seong J.Y.;
RT "Coevolution of the spexin/galanin/kisspeptin family: Spexin activates
RT galanin receptor type II and III.";
RL Endocrinology 155:1864-1873(2014).
RN [3] {ECO:0000305}
RP DEVELOPMENTAL STAGE.
RX PubMed=27315774; DOI=10.1016/j.neulet.2016.06.025;
RA Kim E., Jeong I., Kim S., Kim H.K., Lee D.W., Kim B., Seong J.Y., Bae Y.K.,
RA Ryu J.H., Park H.C.;
RT "Distribution of galanin receptor 2b neurons and interaction with galanin
RT in the zebrafish central nervous system.";
RL Neurosci. Lett. 628:153-160(2016).
RN [4] {ECO:0000305}
RP FUNCTION.
RX PubMed=31474838; DOI=10.3389/fncir.2019.00053;
RA Jeong I., Kim E., Seong J.Y., Park H.C.;
RT "Overexpression of Spexin 1 in the Dorsal Habenula Reduces Anxiety in
RT Zebrafish.";
RL Front. Neural Circuits 13:53-53(2019).
RN [5] {ECO:0000305}
RP DEVELOPMENTAL STAGE.
RX PubMed=30903017; DOI=10.1038/s41598-019-41431-7;
RA Kim E., Jeong I., Chung A.Y., Kim S., Kwon S.H., Seong J.Y., Park H.C.;
RT "Distribution and neuronal circuit of spexin 1/2 neurons in the zebrafish
RT CNS.";
RL Sci. Rep. 9:5025-5025(2019).
CC -!- FUNCTION: Receptor for the hormone galanin (PubMed:24517231). Receptor
CC for the hormones spexin-1 and spexin-2 (PubMed:24517231,
CC PubMed:31474838). {ECO:0000269|PubMed:24517231,
CC ECO:0000269|PubMed:31474838}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in neurons in the ventral area of the
CC interpeduncular nucleus (IPN) where expression often overlaps with
CC spx1. {ECO:0000269|PubMed:30903017}.
CC -!- DEVELOPMENTAL STAGE: In embryos, expressed at low levels in a
CC restricted area of the brain that includes the subpallium.
CC {ECO:0000269|PubMed:27315774}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000305}.
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DR EMBL; AL807798; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BX510998; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; F1R332; -.
DR SMR; F1R332; -.
DR STRING; 7955.ENSDARP00000025567; -.
DR PaxDb; F1R332; -.
DR Ensembl; ENSDART00000023857; ENSDARP00000025567; ENSDARG00000020853.
DR Ensembl; ENSDART00000189972; ENSDARP00000150425; ENSDARG00000113968.
DR ZFIN; ZDB-GENE-130603-37; galr2a.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01050000244983; -.
DR HOGENOM; CLU_009579_6_4_1; -.
DR InParanoid; F1R332; -.
DR OMA; DICTFVF; -.
DR OrthoDB; 1294084at2759; -.
DR TreeFam; TF315737; -.
DR Reactome; R-DRE-375276; Peptide ligand-binding receptors.
DR Reactome; R-DRE-418594; G alpha (i) signalling events.
DR PRO; PR:F1R332; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 12.
DR Bgee; ENSDARG00000020853; Expressed in testis.
DR ExpressionAtlas; F1R332; baseline.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0008528; F:G protein-coupled peptide receptor activity; IBA:GO_Central.
DR GO; GO:0004966; F:galanin receptor activity; IDA:ZFIN.
DR GO; GO:0007188; P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IBA:GO_Central.
DR InterPro; IPR000405; Galanin_rcpt.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00663; GALANINR.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; G-protein coupled receptor; Membrane; Receptor;
KW Reference proteome; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..363
FT /note="Galanin receptor 2a"
FT /id="PRO_0000448936"
FT TOPO_DOM 1..23
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 24..44
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 45..54
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 55..75
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 76..94
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 95..115
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 116..135
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 136..156
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 157..179
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 180..200
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 201..230
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 231..251
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 252..268
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 269..289
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 290..363
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT DISULFID 92..169
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ SEQUENCE 363 AA; 41329 MW; 70B3A20F24E63C8B CRC64;
MNASQQIHVF SSHWKVESVI ISLIFSMIFL VGTVGNCLVL AVLIRNGQMN TKSTNLFILN
LGLADLCFIV FCVPLQATIY TMDEWVFGAF VCKAVHFIIY LTMYASIFTL AAVSLDRYLA
IRYPLRSRET RTPRNALTSI SLVWALSLFF SSPYLSYYQQ MDLDGTTVCI PAWSVHHRQA
MDICTFIFGY LIPVLILGIT YARTIRYLWT SVDPMQDMSE SRKAKRKVTK MIIIVAVLFC
LCWLPHHLVI LCMWFGHFPL NHTTYVLRIL SHLVAYANSC LNPIVYALVS KHFRKGFKKV
FGCAFRNRVV NRIHTVQPAQ TVSLMEAASS EGSNHCDGST RGRLWSKSSK KMMTSAFMTF
NVT
