GAL2B_DANRE
ID GAL2B_DANRE Reviewed; 378 AA.
AC E7F7V7;
DT 26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT 08-MAR-2011, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Galanin receptor 2b {ECO:0000303|PubMed:24517231};
GN Name=galr2b {ECO:0000303|PubMed:24517231,
GN ECO:0000312|ZFIN:ZDB-GENE-131121-48};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000312|Proteomes:UP000000437};
RN [1] {ECO:0000312|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen {ECO:0000312|Proteomes:UP000000437};
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2] {ECO:0000305}
RP FUNCTION.
RX PubMed=24517231; DOI=10.1210/en.2013-2106;
RA Kim D.K., Yun S., Son G.H., Hwang J.I., Park C.R., Kim J.I., Kim K.,
RA Vaudry H., Seong J.Y.;
RT "Coevolution of the spexin/galanin/kisspeptin family: Spexin activates
RT galanin receptor type II and III.";
RL Endocrinology 155:1864-1873(2014).
RN [3] {ECO:0000305}
RP DEVELOPMENTAL STAGE.
RX PubMed=27315774; DOI=10.1016/j.neulet.2016.06.025;
RA Kim E., Jeong I., Kim S., Kim H.K., Lee D.W., Kim B., Seong J.Y., Bae Y.K.,
RA Ryu J.H., Park H.C.;
RT "Distribution of galanin receptor 2b neurons and interaction with galanin
RT in the zebrafish central nervous system.";
RL Neurosci. Lett. 628:153-160(2016).
RN [4] {ECO:0000305}
RP FUNCTION.
RX PubMed=31474838; DOI=10.3389/fncir.2019.00053;
RA Jeong I., Kim E., Seong J.Y., Park H.C.;
RT "Overexpression of Spexin 1 in the Dorsal Habenula Reduces Anxiety in
RT Zebrafish.";
RL Front. Neural Circuits 13:53-53(2019).
RN [5] {ECO:0000305}
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=30903017; DOI=10.1038/s41598-019-41431-7;
RA Kim E., Jeong I., Chung A.Y., Kim S., Kwon S.H., Seong J.Y., Park H.C.;
RT "Distribution and neuronal circuit of spexin 1/2 neurons in the zebrafish
RT CNS.";
RL Sci. Rep. 9:5025-5025(2019).
CC -!- FUNCTION: Receptor for the hormone galanin (PubMed:24517231). Receptor
CC for the hormones spexin-1 and spexin-2 (PubMed:24517231,
CC PubMed:31474838). {ECO:0000269|PubMed:24517231,
CC ECO:0000269|PubMed:31474838}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in neurons in the ventral area of the
CC interpeduncular nucleus (IPN) where expression often overlaps with
CC spx1. {ECO:0000269|PubMed:30903017}.
CC -!- DEVELOPMENTAL STAGE: In embryos, widely expressed in nerve cells
CC innervating the central nervous system (CNS) in regions including the
CC olfactory bulb, midbrain tegmentum, preoptic region, dorsal thalamus,
CC posterior tuberculum, postoptic commissure, hindbrain, and lateral
CC margin of the spinal cord (PubMed:27315774). In the spinal cord,
CC expressed in various neuronal cell types throughout the dorsoventral
CC axis, including motor neurons and interneurons (PubMed:27315774). In
CC the postoptic commissure, anterior commissure, hypothalamus and
CC pituitary gland, expression overlaps with gal (PubMed:27315774). In
CC larvae, expressed in nerve cells in the dorsal spinal cord where
CC expression often overlaps with spx1 (PubMed:30903017).
CC {ECO:0000269|PubMed:27315774, ECO:0000269|PubMed:30903017}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000305}.
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DR EMBL; CU681846; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_001339169.1; XM_001339133.4.
DR AlphaFoldDB; E7F7V7; -.
DR SMR; E7F7V7; -.
DR STRING; 7955.ENSDARP00000128014; -.
DR PaxDb; E7F7V7; -.
DR Ensembl; ENSDART00000164581; ENSDARP00000140272; ENSDARG00000105113.
DR Ensembl; ENSDART00000188300; ENSDARP00000150204; ENSDARG00000105113.
DR GeneID; 798737; -.
DR KEGG; dre:798737; -.
DR CTD; 798737; -.
DR ZFIN; ZDB-GENE-131121-48; galr2b.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01050000244841; -.
DR HOGENOM; CLU_009579_6_4_1; -.
DR InParanoid; E7F7V7; -.
DR OMA; TEVSQMH; -.
DR OrthoDB; 1294084at2759; -.
DR PhylomeDB; E7F7V7; -.
DR TreeFam; TF315737; -.
DR Reactome; R-DRE-375276; Peptide ligand-binding receptors.
DR Reactome; R-DRE-418594; G alpha (i) signalling events.
DR PRO; PR:E7F7V7; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 3.
DR Bgee; ENSDARG00000105113; Expressed in interpeduncular nucleus and 15 other tissues.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0008528; F:G protein-coupled peptide receptor activity; IBA:GO_Central.
DR GO; GO:0004966; F:galanin receptor activity; IDA:ZFIN.
DR GO; GO:0007188; P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IBA:GO_Central.
DR InterPro; IPR000405; Galanin_rcpt.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00663; GALANINR.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; G-protein coupled receptor; Membrane; Receptor;
KW Reference proteome; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..378
FT /note="Galanin receptor 2b"
FT /id="PRO_0000448937"
FT TOPO_DOM 1..30
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 31..51
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 52..62
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 63..83
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 84..101
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 102..123
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 124..143
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 144..164
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 165..187
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 188..208
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 209..238
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 239..259
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 260..276
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 277..297
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 298..378
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT REGION 339..362
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 100..177
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ SEQUENCE 378 AA; 42767 MW; AF0225C78446549B CRC64;
MSDHEDLNKA MGHWNASESY QLNPASVIVS VVFSLIFLLG TIGNSLVLAV LLRSGQVGYN
TTNLFILNLS VADFFFIIFC VPFQATIYSL EGWVFGSFMC KVVHFFINLT MYASSFTLAA
VSVDRYLAIR YPLRSRELRT PCNAVVAMVV IWGLSLVFAG PYLSYYDLID FENSNVCVPG
WEEHNRKVLD TCTFVFGYVI PVLIVSLSYT RTIKYLWTAV DPLDGMSESK RAKRKVTKMI
IIVTVLFCIC WLPYHVVILC YLYGDFPFNQ TTYAFRLLSH CMAYANSCLN PIVYALVSKH
FRKGFKKVFS CILSKKGRNK VHVVNVANTV PGFEAGSTEV SQMNEENARQ NESEMVNRPL
AQPQDTTMTI TLPFQNQP
