GAL3_YEAST
ID GAL3_YEAST Reviewed; 520 AA.
AC P13045; D6VRZ6;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 2.
DT 03-AUG-2022, entry version 188.
DE RecName: Full=Protein GAL3;
GN Name=GAL3; OrderedLocusNames=YDR009W; ORFNames=YD8119.14;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-425.
RX PubMed=3062381; DOI=10.1128/mcb.8.8.3439-3447.1988;
RA Bajwa W., Torchia T.E., Hopper J.E.;
RT "Yeast regulatory gene GAL3: carbon regulation; UASGal elements in common
RT with GAL1, GAL2, GAL7, GAL10, GAL80, and MEL1; encoded protein strikingly
RT similar to yeast and Escherichia coli galactokinases.";
RL Mol. Cell. Biol. 8:3439-3447(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8896275;
RX DOI=10.1002/(sici)1097-0061(199609)12:10b<1085::aid-yea9>3.3.co;2-s;
RA Eide L.G., Sander C., Prydz H.;
RT "Sequencing and analysis of a 35.4 kb region on the right arm of chromosome
RT IV from Saccharomyces cerevisiae reveal 23 open reading frames.";
RL Yeast 12:1085-1090(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: The GAL3 regulatory function is required for rapid induction
CC of the galactose system. At normal induction, galactose in the presence
CC of the GAL3 protein may lead to the induction of the GAL genes,
CC including GAL1. Then the galactokinase protein (GAL1) in the presence
CC of galactose may reinforce the induction leading to a higher expression
CC level. Upon depletion of galactose, the inducing activity of the
CC galactokinase protein may decrease, after which transcription of the
CC GAL genes, including GAL1, may decrease.
CC -!- INTERACTION:
CC P13045; P04387: GAL80; NbExp=5; IntAct=EBI-7282, EBI-2061197;
CC -!- INDUCTION: The GAL3 gene is a member of the family of galactose
CC inducible, glucose-repressible genes (which include GAL1, GAL2, GAL7,
CC GAL10, GAL80, and MELI).
CC -!- MISCELLANEOUS: Present with 721 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the GHMP kinase family. GalK subfamily.
CC {ECO:0000305}.
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DR EMBL; M21615; AAA34625.1; -; Genomic_DNA.
DR EMBL; X95966; CAA65201.1; -; Genomic_DNA.
DR EMBL; Z48008; CAA88069.1; -; Genomic_DNA.
DR EMBL; Z74305; CAA98829.1; -; Genomic_DNA.
DR EMBL; AY723766; AAU09683.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11856.1; -; Genomic_DNA.
DR PIR; S50990; S50990.
DR RefSeq; NP_010292.1; NM_001180317.1.
DR PDB; 3V2U; X-ray; 2.10 A; C/D=2-520.
DR PDB; 3V5R; X-ray; 2.10 A; A/B=17-520.
DR PDBsum; 3V2U; -.
DR PDBsum; 3V5R; -.
DR AlphaFoldDB; P13045; -.
DR SMR; P13045; -.
DR BioGRID; 32061; 64.
DR ComplexPortal; CPX-1042; GAL3-GAL80 transcription regulation complex.
DR DIP; DIP-5725N; -.
DR IntAct; P13045; 4.
DR MINT; P13045; -.
DR STRING; 4932.YDR009W; -.
DR iPTMnet; P13045; -.
DR MaxQB; P13045; -.
DR PaxDb; P13045; -.
DR PRIDE; P13045; -.
DR EnsemblFungi; YDR009W_mRNA; YDR009W; YDR009W.
DR GeneID; 851572; -.
DR KEGG; sce:YDR009W; -.
DR SGD; S000002416; GAL3.
DR VEuPathDB; FungiDB:YDR009W; -.
DR eggNOG; KOG0631; Eukaryota.
DR GeneTree; ENSGT00950000183187; -.
DR HOGENOM; CLU_017814_6_2_1; -.
DR InParanoid; P13045; -.
DR OMA; HQEIDLQ; -.
DR BioCyc; YEAST:G3O-29629-MON; -.
DR PRO; PR:P13045; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; P13045; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0005667; C:transcription regulator complex; IC:ComplexPortal.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004335; F:galactokinase activity; IBA:GO_Central.
DR GO; GO:0006012; P:galactose metabolic process; IMP:SGD.
DR GO; GO:0045185; P:maintenance of protein location; IMP:SGD.
DR GO; GO:0000435; P:positive regulation of transcription from RNA polymerase II promoter by galactose; IMP:SGD.
DR Gene3D; 3.30.230.10; -; 1.
DR InterPro; IPR000705; Galactokinase.
DR InterPro; IPR019741; Galactokinase_CS.
DR InterPro; IPR019539; GalKase_N.
DR InterPro; IPR013750; GHMP_kinase_C_dom.
DR InterPro; IPR036554; GHMP_kinase_C_sf.
DR InterPro; IPR006204; GHMP_kinase_N_dom.
DR InterPro; IPR006206; Mevalonate/galactokinase.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR Pfam; PF10509; GalKase_gal_bdg; 1.
DR Pfam; PF08544; GHMP_kinases_C; 1.
DR Pfam; PF00288; GHMP_kinases_N; 1.
DR PIRSF; PIRSF000530; Galactokinase; 1.
DR PRINTS; PR00473; GALCTOKINASE.
DR SUPFAM; SSF54211; SSF54211; 1.
DR SUPFAM; SSF55060; SSF55060; 1.
DR TIGRFAMs; TIGR00131; gal_kin; 1.
DR PROSITE; PS00106; GALACTOKINASE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; ATP-binding; Carbohydrate metabolism;
KW Galactose metabolism; Nucleotide-binding; Reference proteome.
FT CHAIN 1..520
FT /note="Protein GAL3"
FT /id="PRO_0000184658"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CONFLICT 37..45
FT /note="KPDFIARSP -> NLILSLGLL (in Ref. 1; AAA34625)"
FT /evidence="ECO:0000305"
FT CONFLICT 170..171
FT /note="AA -> GR (in Ref. 1; AAA34625)"
FT /evidence="ECO:0000305"
FT CONFLICT 192..211
FT /note="RITAVAEHYVGVNNGGMDQA -> ASQRLRSTMLESIMVVWIKQ (in
FT Ref. 1; AAA34625)"
FT /evidence="ECO:0000305"
FT CONFLICT 227..241
FT /note="RPKLKATPFKFPQLK -> MAKTKWPHFQVSSIE (in Ref. 1;
FT AAA34625)"
FT /evidence="ECO:0000305"
FT CONFLICT 251..294
FT /note="NTLVKSNKFETAPTNYNLRVIEVTVAANALATRYSVALPSHKDN -> ILCT
FT RSNNRTLLHISLSCSLLTLLYISTFAREPCGPDTLGLTISQGQ (in Ref. 1;
FT AAA34625)"
FT /evidence="ECO:0000305"
FT CONFLICT 303..338
FT /note="RDFMDAYYARYENQAQPWNGDIGTGIERLLKMLQLV -> EIYGCLLRPDTK
FT TKPNHGMEISELVLNVYSRCYNWY (in Ref. 1; AAA34625)"
FT /evidence="ECO:0000305"
FT CONFLICT 407..425
FT /note="TFHTDEDFFTDFGRLMNES -> IFTRTRFLYRFWPTNE (in Ref. 1;
FT AAA34625)"
FT /evidence="ECO:0000305"
FT STRAND 7..9
FT /evidence="ECO:0007829|PDB:3V2U"
FT HELIX 20..34
FT /evidence="ECO:0007829|PDB:3V5R"
FT STRAND 39..51
FT /evidence="ECO:0007829|PDB:3V5R"
FT HELIX 56..58
FT /evidence="ECO:0007829|PDB:3V5R"
FT STRAND 62..77
FT /evidence="ECO:0007829|PDB:3V5R"
FT STRAND 84..91
FT /evidence="ECO:0007829|PDB:3V5R"
FT STRAND 97..100
FT /evidence="ECO:0007829|PDB:3V5R"
FT STRAND 112..114
FT /evidence="ECO:0007829|PDB:3V5R"
FT HELIX 119..135
FT /evidence="ECO:0007829|PDB:3V5R"
FT HELIX 137..140
FT /evidence="ECO:0007829|PDB:3V5R"
FT STRAND 141..143
FT /evidence="ECO:0007829|PDB:3V5R"
FT STRAND 148..154
FT /evidence="ECO:0007829|PDB:3V5R"
FT HELIX 162..179
FT /evidence="ECO:0007829|PDB:3V5R"
FT HELIX 187..195
FT /evidence="ECO:0007829|PDB:3V5R"
FT HELIX 198..201
FT /evidence="ECO:0007829|PDB:3V5R"
FT HELIX 208..215
FT /evidence="ECO:0007829|PDB:3V5R"
FT STRAND 220..225
FT /evidence="ECO:0007829|PDB:3V5R"
FT STRAND 227..229
FT /evidence="ECO:0007829|PDB:3V5R"
FT STRAND 231..236
FT /evidence="ECO:0007829|PDB:3V5R"
FT STRAND 244..251
FT /evidence="ECO:0007829|PDB:3V5R"
FT HELIX 258..261
FT /evidence="ECO:0007829|PDB:3V2U"
FT TURN 263..265
FT /evidence="ECO:0007829|PDB:3V5R"
FT HELIX 266..283
FT /evidence="ECO:0007829|PDB:3V5R"
FT STRAND 296..299
FT /evidence="ECO:0007829|PDB:3V5R"
FT HELIX 302..313
FT /evidence="ECO:0007829|PDB:3V5R"
FT HELIX 324..342
FT /evidence="ECO:0007829|PDB:3V5R"
FT HELIX 343..345
FT /evidence="ECO:0007829|PDB:3V5R"
FT HELIX 351..357
FT /evidence="ECO:0007829|PDB:3V5R"
FT HELIX 362..369
FT /evidence="ECO:0007829|PDB:3V5R"
FT STRAND 370..373
FT /evidence="ECO:0007829|PDB:3V5R"
FT STRAND 378..380
FT /evidence="ECO:0007829|PDB:3V5R"
FT HELIX 382..404
FT /evidence="ECO:0007829|PDB:3V5R"
FT HELIX 411..431
FT /evidence="ECO:0007829|PDB:3V5R"
FT HELIX 438..449
FT /evidence="ECO:0007829|PDB:3V5R"
FT STRAND 453..473
FT /evidence="ECO:0007829|PDB:3V5R"
FT HELIX 478..488
FT /evidence="ECO:0007829|PDB:3V5R"
FT HELIX 490..493
FT /evidence="ECO:0007829|PDB:3V5R"
FT HELIX 499..505
FT /evidence="ECO:0007829|PDB:3V5R"
FT STRAND 506..509
FT /evidence="ECO:0007829|PDB:3V5R"
FT STRAND 515..519
FT /evidence="ECO:0007829|PDB:3V5R"
SQ SEQUENCE 520 AA; 58129 MW; 5852028EBDDC0F3B CRC64;
MNTNVPIFSS PVRDLPRSFE QKHLAVVDAF FQTYHVKPDF IARSPGRVNL IGEHIDYCDF
SVLPLAIDVD MLCAVKILDE KNPSITLTNA DPKFAQRKFD LPLDGSYMAI DPSVSEWSNY
FKCGLHVAHS YLKKIAPERF NNTPLVGAQI FCQSDIPTGG GLSSAFTCAA ALATIRANMG
KNFDISKKDL TRITAVAEHY VGVNNGGMDQ ATSVYGEEDH ALYVEFRPKL KATPFKFPQL
KNHEISFVIA NTLVKSNKFE TAPTNYNLRV IEVTVAANAL ATRYSVALPS HKDNSNSERG
NLRDFMDAYY ARYENQAQPW NGDIGTGIER LLKMLQLVEE SFSRKKSGFT VHEASTALNC
SREEFTRDYL TTFPVRFQVL KLYQRAKHVY SESLRVLKAL KMMTSATFHT DEDFFTDFGR
LMNESQASCD KLYECSCIET NQICSIALAN GSFGSRLTGA GWGGCTIHLV PSGANGNVEQ
VRKALIEKFY NVRYPDLTDE ELKDAIIVSK PALGTCLYEQ
