ID   GALAB_RHIME             Reviewed;         294 AA.
AC   Q92RN9;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Putative sugar lactone lactonase;
DE            EC=3.1.1.-;
GN   OrderedLocusNames=R00823; ORFNames=SMc00883;
OS   Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS   meliloti).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=266834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11481430; DOI=10.1073/pnas.161294398;
RA   Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA   Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA   Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA   Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA   Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT   "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT   meliloti strain 1021.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11474104; DOI=10.1126/science.1060966;
RA   Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA   Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA   Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA   Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA   Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA   Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA   Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA   Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA   Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA   Wong K., Yeh K.-C., Batut J.;
RT   "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL   Science 293:668-672(2001).
RN   [3]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=1021;
RX   PubMed=22797764; DOI=10.1128/jb.00982-12;
RA   Geddes B.A., Oresnik I.J.;
RT   "Inability to catabolize galactose leads to increased ability to compete
RT   for nodule occupancy in Sinorhizobium meliloti.";
RL   J. Bacteriol. 194:5044-5053(2012).
CC   -!- FUNCTION: Involved in the degradation of galactose via the DeLey-
CC       Doudoroff pathway. {ECO:0000305}.
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000250};
CC       Note=Binds 1 divalent metal cation per subunit. {ECO:0000250};
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene exhibit no detectable
CC       phenotype when grown on galactose as sole carbon source.
CC       {ECO:0000269|PubMed:22797764}.
CC   -!- SIMILARITY: Belongs to the SMP-30/CGR1 family. {ECO:0000305}.
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DR   EMBL; AL591688; CAC45395.1; -; Genomic_DNA.
DR   RefSeq; NP_384929.1; NC_003047.1.
DR   RefSeq; WP_010968848.1; NC_003047.1.
DR   AlphaFoldDB; Q92RN9; -.
DR   SMR; Q92RN9; -.
DR   STRING; 266834.SMc00883; -.
DR   EnsemblBacteria; CAC45395; CAC45395; SMc00883.
DR   GeneID; 61602289; -.
DR   KEGG; sme:SMc00883; -.
DR   PATRIC; fig|266834.11.peg.2213; -.
DR   eggNOG; COG3386; Bacteria.
DR   HOGENOM; CLU_036110_3_1_5; -.
DR   OMA; WTDIEGA; -.
DR   Proteomes; UP000001976; Chromosome.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 2.120.10.30; -; 1.
DR   InterPro; IPR011042; 6-blade_b-propeller_TolB-like.
DR   InterPro; IPR013658; SGL.
DR   InterPro; IPR005511; SMP-30.
DR   Pfam; PF08450; SGL; 1.
DR   PRINTS; PR01790; SMP30FAMILY.
PE   3: Inferred from homology;
KW   Hydrolase; Metal-binding; Reference proteome.
FT   CHAIN           1..294
FT                   /note="Putative sugar lactone lactonase"
FT                   /id="PRO_0000428932"
FT   ACT_SITE        201
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         21
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000250"
FT   BINDING         150
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000250"
FT   BINDING         201
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   294 AA;  31370 MW;  BF2A2A28DCF795D6 CRC64;
     MTDLVPFSGR TLSEAASELG EGPTFDPGTG TAWWFNITGR ELHELHLESG RKAIHPLPFL
     GSVLAVIDPL RQLIASDQGL FVRDTESSKL GHFATLEEKP GNRSNDGRIH PCGALWIGTM
     GRSAEKHAGA IYHVAGSRVT KLYSNITIPN AICFSPDGAT AYFTDTDVNQ LMRVDIDPAT
     ALPTGDPVLL SDESTSPGGV DGAVCDADGL IWNARWGASA VEVYKPDGQK VARYAVPATQ
     PSCPAFVGAK AERLLVTSAW QGMDDAARAA DPHAGKTFEL GIEVKGRFEP AFRL