ID   GALD_PSEPK              Reviewed;         361 AA.
AC   Q88JY0;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=4-oxalomesaconate tautomerase;
DE            EC=5.3.2.8;
DE   AltName: Full=Gallate degradation protein D;
GN   Name=galD; OrderedLocusNames=PP_2513;
OS   Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS   / KT2440).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=160488;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX   PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA   Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA   Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA   Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA   Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M., Hance I.,
RA   Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A.,
RA   Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H.,
RA   Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C.,
RA   Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.;
RT   "Complete genome sequence and comparative analysis of the metabolically
RT   versatile Pseudomonas putida KT2440.";
RL   Environ. Microbiol. 4:799-808(2002).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, PATHWAY, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX   PubMed=21219457; DOI=10.1111/j.1365-2958.2010.07448.x;
RA   Nogales J., Canales A., Jimenez-Barbero J., Serra B., Pingarron J.M.,
RA   Garcia J.L., Diaz E.;
RT   "Unravelling the gallic acid degradation pathway in bacteria: the gal
RT   cluster from Pseudomonas putida.";
RL   Mol. Microbiol. 79:359-374(2011).
CC   -!- FUNCTION: Catalyzes the tautomerization of the 4-oxalomesaconic acid
CC       keto (OMAketo) generated by GalA dioxygenase to 4-oxalomesaconic acid
CC       enol (OMAenol). Mediates the second step in gallate degradation
CC       pathway. {ECO:0000269|PubMed:21219457}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(1E)-4-oxobut-1-ene-1,2,4-tricarboxylate = 4-carboxy-2-
CC         hydroxy-cis,cis-muconate; Xref=Rhea:RHEA:28931, ChEBI:CHEBI:57471,
CC         ChEBI:CHEBI:58142; EC=5.3.2.8;
CC         Evidence={ECO:0000269|PubMed:21219457};
CC   -!- DISRUPTION PHENOTYPE: Does not prevent growth in 5 mM gallate medium,
CC       but the mutant strain grows poorly at gallate concentrations lower than
CC       2 mM. {ECO:0000269|PubMed:21219457}.
CC   -!- SIMILARITY: Belongs to the PrpF family. {ECO:0000305}.
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DR   EMBL; AE015451; AAN68125.1; -; Genomic_DNA.
DR   RefSeq; NP_744661.1; NC_002947.4.
DR   RefSeq; WP_010953447.1; NC_002947.4.
DR   AlphaFoldDB; Q88JY0; -.
DR   SMR; Q88JY0; -.
DR   STRING; 160488.PP_2513; -.
DR   PRIDE; Q88JY0; -.
DR   EnsemblBacteria; AAN68125; AAN68125; PP_2513.
DR   KEGG; ppu:PP_2513; -.
DR   PATRIC; fig|160488.4.peg.2668; -.
DR   eggNOG; COG2828; Bacteria.
DR   HOGENOM; CLU_026443_2_1_6; -.
DR   OMA; TCIDVGN; -.
DR   PhylomeDB; Q88JY0; -.
DR   BioCyc; MetaCyc:G1G01-2697-MON; -.
DR   BioCyc; PPUT160488:G1G01-2697-MON; -.
DR   Proteomes; UP000000556; Chromosome.
DR   GO; GO:0016863; F:intramolecular oxidoreductase activity, transposing C=C bonds; IDA:UniProtKB.
DR   GO; GO:0019396; P:gallate catabolic process; IDA:UniProtKB.
DR   InterPro; IPR007400; PrpF_protein.
DR   PANTHER; PTHR43709; PTHR43709; 1.
DR   Pfam; PF04303; PrpF; 1.
PE   1: Evidence at protein level;
KW   Aromatic hydrocarbons catabolism; Isomerase; Reference proteome.
FT   CHAIN           1..361
FT                   /note="4-oxalomesaconate tautomerase"
FT                   /id="PRO_0000418496"
SQ   SEQUENCE   361 AA;  37559 MW;  0938CFBFEE66F467 CRC64;
     MGQTRIPCLL MRGGTSKGAY FLHDDLPAPG PLRDRVLLAV MGSPDARQID GIGGADSLTS
     KVAIIRASQR DDADVDYLFA QVVVDEARVD YGQNCGNILA GVGPFALERG LVAASGASTP
     VRIFMENTGQ IAVAQVPTAD GQVEYAGDTR IDGVPGRAAA LVVTFADVAG ASCGALLPTG
     NSRDCVEGVE VTCIDNGMPV VLLCAEDLGV TGYEPCETLE ADSALKTRLE AIRLQLGPRM
     NLGDVSQRNV PKMCLLSAPR NGGTVNTRSF IPHRCHASIG VFGAVSVATA CLIEGSVAQG
     LASTSGGDRQ RLAVEHPSGE FTVEISLEHG VIKGCGLVRT ARLLFDGVVC IGRDTWGGPE
     K