ID   GALD_RHIME              Reviewed;         256 AA.
AC   Q92RN6;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Probable galactose dehydrogenase GalD;
DE            EC=1.1.1.-;
GN   Name=galD; Synonyms=fabG2; OrderedLocusNames=R00826; ORFNames=SMc00880;
OS   Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS   meliloti).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=266834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11481430; DOI=10.1073/pnas.161294398;
RA   Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA   Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA   Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA   Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA   Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT   "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT   meliloti strain 1021.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11474104; DOI=10.1126/science.1060966;
RA   Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA   Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA   Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA   Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA   Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA   Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA   Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA   Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA   Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA   Wong K., Yeh K.-C., Batut J.;
RT   "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL   Science 293:668-672(2001).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=1021;
RX   PubMed=22797764; DOI=10.1128/jb.00982-12;
RA   Geddes B.A., Oresnik I.J.;
RT   "Inability to catabolize galactose leads to increased ability to compete
RT   for nodule occupancy in Sinorhizobium meliloti.";
RL   J. Bacteriol. 194:5044-5053(2012).
CC   -!- FUNCTION: Involved in the degradation of galactose via the DeLey-
CC       Doudoroff pathway. Catalyzes the oxidation of galactose in the presence
CC       of NAD(+). Uses NAD(+) as a hydrogen acceptor more efficiently than
CC       NADP(+). {ECO:0000269|PubMed:22797764}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene fail to grow on galactose
CC       as sole carbon source. {ECO:0000269|PubMed:22797764}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; AL591688; CAC45398.1; -; Genomic_DNA.
DR   RefSeq; NP_384932.1; NC_003047.1.
DR   RefSeq; WP_010968851.1; NC_003047.1.
DR   AlphaFoldDB; Q92RN6; -.
DR   SMR; Q92RN6; -.
DR   STRING; 266834.SMc00880; -.
DR   EnsemblBacteria; CAC45398; CAC45398; SMc00880.
DR   GeneID; 61602292; -.
DR   KEGG; sme:SMc00880; -.
DR   PATRIC; fig|266834.11.peg.2216; -.
DR   eggNOG; COG1028; Bacteria.
DR   HOGENOM; CLU_010194_1_0_5; -.
DR   OMA; FSSISYM; -.
DR   Proteomes; UP000001976; Chromosome.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   3: Inferred from homology;
KW   NAD; Oxidoreductase; Reference proteome.
FT   CHAIN           1..256
FT                   /note="Probable galactose dehydrogenase GalD"
FT                   /id="PRO_0000428929"
FT   ACT_SITE        163
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT   BINDING         20..23
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         71..72
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         98
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         150
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         163..167
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         196
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   256 AA;  27294 MW;  B5A44F4D0E3C5E90 CRC64;
     MTLPSSQFPD LRDRGVLVTG GGSGIGAALV EAFARQGARV AFVDIAAESS LALCEKVAAQ
     TGQAPHFIQA DLRNVEAVRA AADEAVAKLG SVRVLVNNAA RDDRQALEAV TEESWDESLS
     VNLRHLFFMC QAVAPHMQRQ GGGSIVNFSS IAFLLNMPEI PAYSTAKAGI IGLTKSLAGK
     LGPDNIRVNA ILPGMIVTER QRRLWLTEES IARMQERQCL KRMLVADDLV GPCLFLASDS
     SAAMTAQAMI IDGGVF