ID   GALE1_CYATE             Reviewed;         354 AA.
AC   O65780;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=UDP-glucose 4-epimerase GEPI42;
DE            EC=5.1.3.2;
DE   AltName: Full=Galactowaldenase;
DE   AltName: Full=UDP-galactose 4-epimerase;
OS   Cyamopsis tetragonoloba (Guar) (Cluster bean).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Indigofereae; Cyamopsis.
OX   NCBI_TaxID=3832;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Brunstedt J., Joersbo M., Pedersen S.G., Marcussen J.;
RT   "Isolation and expression of two cDNA clones encoding UDP-galactose
RT   epimerase genes expressed in guar endosperm.";
RL   Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=UDP-alpha-D-glucose = UDP-alpha-D-galactose;
CC         Xref=Rhea:RHEA:22168, ChEBI:CHEBI:58885, ChEBI:CHEBI:66914;
CC         EC=5.1.3.2;
CC   -!- COFACTOR:
CC       Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250};
CC   -!- PATHWAY: Carbohydrate metabolism; galactose metabolism.
CC   -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC       family. {ECO:0000305}.
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DR   EMBL; AJ005081; CAA06338.1; -; mRNA.
DR   PIR; T10496; T10496.
DR   AlphaFoldDB; O65780; -.
DR   SMR; O65780; -.
DR   PRIDE; O65780; -.
DR   UniPathway; UPA00214; -.
DR   GO; GO:0003978; F:UDP-glucose 4-epimerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006012; P:galactose metabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd05247; UDP_G4E_1_SDR_e; 1.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR005886; UDP_G4E.
DR   Pfam; PF16363; GDP_Man_Dehyd; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR01179; galE; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Galactose metabolism; Isomerase; NAD.
FT   CHAIN           1..354
FT                   /note="UDP-glucose 4-epimerase GEPI42"
FT                   /id="PRO_0000183196"
FT   ACT_SITE        161
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         11..42
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         137
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   354 AA;  39286 MW;  1C9DD724F53FE3B1 CRC64;
     MVSSRMASGE TILVTGGAGF IGSHTVVQLL KQGFHVSIID NLYNSVIDAV HRVRLLVGPL
     LSSNLHFHHG DLRNIHDLDI LFSKTKFDAV IHFAGLKGVG ESVLNPSNYY DNNLVATINL
     FQVMSKFNCK KLVISSSATV YGQPDQIPCV EDSNLHAMNP YGRSKLFVEE VARDIQRAEA
     EWRIILLRYF NPVGAHESGQ IGEDPRGLPN NLMPYIQQVA VARLPELNIY GHDYPTKDGT
     AIRDYIHVMD LADGHIAALR KLFTTDNIGC TAYNLGTGRG TSVLEMVAAF EKASGKKIPI
     KMCPRRPGDA TAVYASTEKA EKELGWKAKY GVEEMCRDQW KWASNNPWGY QGKH