ID   GALE1_PEA               Reviewed;         350 AA.
AC   Q43070;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=UDP-glucose 4-epimerase;
DE            EC=5.1.3.2;
DE   AltName: Full=Galactowaldenase;
DE   AltName: Full=UDP-galactose 4-epimerase;
GN   Name=GALE;
OS   Pisum sativum (Garden pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Wando;
RA   Slocum R.D., Lake M.R., Williamson C.L.;
RT   "Cloning and characterization of a UDP-galactose-4-epimerase
RT   ('galactowaldenase') and its expression in pea tissues.";
RL   Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=UDP-alpha-D-glucose = UDP-alpha-D-galactose;
CC         Xref=Rhea:RHEA:22168, ChEBI:CHEBI:58885, ChEBI:CHEBI:66914;
CC         EC=5.1.3.2;
CC   -!- COFACTOR:
CC       Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250};
CC   -!- PATHWAY: Carbohydrate metabolism; galactose metabolism.
CC   -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC       family. {ECO:0000305}.
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DR   EMBL; U31544; AAA86532.1; -; mRNA.
DR   PIR; T06526; T06526.
DR   AlphaFoldDB; Q43070; -.
DR   SMR; Q43070; -.
DR   UniPathway; UPA00214; -.
DR   GO; GO:0003978; F:UDP-glucose 4-epimerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006012; P:galactose metabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd05247; UDP_G4E_1_SDR_e; 1.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR005886; UDP_G4E.
DR   Pfam; PF16363; GDP_Man_Dehyd; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR01179; galE; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Galactose metabolism; Isomerase; NAD.
FT   CHAIN           1..350
FT                   /note="UDP-glucose 4-epimerase"
FT                   /id="PRO_0000183198"
FT   ACT_SITE        157
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         7..38
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         133
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   350 AA;  38990 MW;  86CB3444C9BDCFAC CRC64;
     MVASSQKILV TGSAGFIGTH TVVQLLNNGF NVSIIDNFDN SVMEAVERVR EVVGSNLSQN
     LEFTLGDLRN KDDLEKLFSK SKFDAVIHFA GLKAVGESVE NPRRYFDNNL VGTINLYEVM
     AKHNCKKMVF SSSATVYGQP EKIPCVEDFK LQAMNPYGRT KLFLEEIARD IQKAEPEWRI
     VLLRYFNPVG AHESGKLGED PRGIPNNLMP YIQQVAVGRL PELNVYGHDY PTRDGSAIRD
     YIHVMDLADG HIAALRKLFT SENIGCTAYN LGTGRGSSVL EMVAAFEKAS GKKIALKLCP
     RRPGDATEVY ASTAKAEKEL GWKAKYGVEE MCRDQWNWAK NNPWGYSGKP