ID   GALE2_CYATE             Reviewed;         350 AA.
AC   O65781;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=UDP-glucose 4-epimerase GEPI48;
DE            EC=5.1.3.2;
DE   AltName: Full=Galactowaldenase;
DE   AltName: Full=UDP-galactose 4-epimerase;
OS   Cyamopsis tetragonoloba (Guar) (Cluster bean).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Indigofereae; Cyamopsis.
OX   NCBI_TaxID=3832;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Brunstedt J., Joersbo M., Pedersen S.G., Marcussen J.;
RT   "Isolation and expression of two cDNA clones encoding UDP-galactose
RT   epimerase genes expressed in guar endosperm.";
RL   Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=UDP-alpha-D-glucose = UDP-alpha-D-galactose;
CC         Xref=Rhea:RHEA:22168, ChEBI:CHEBI:58885, ChEBI:CHEBI:66914;
CC         EC=5.1.3.2;
CC   -!- COFACTOR:
CC       Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250};
CC   -!- PATHWAY: Carbohydrate metabolism; galactose metabolism.
CC   -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC       family. {ECO:0000305}.
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DR   EMBL; AJ005082; CAA06339.1; -; mRNA.
DR   PIR; T10498; T10498.
DR   AlphaFoldDB; O65781; -.
DR   SMR; O65781; -.
DR   UniPathway; UPA00214; -.
DR   GO; GO:0003978; F:UDP-glucose 4-epimerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006012; P:galactose metabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd05247; UDP_G4E_1_SDR_e; 1.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR005886; UDP_G4E.
DR   Pfam; PF16363; GDP_Man_Dehyd; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR01179; galE; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Galactose metabolism; Isomerase; NAD.
FT   CHAIN           1..350
FT                   /note="UDP-glucose 4-epimerase GEPI48"
FT                   /id="PRO_0000183197"
FT   ACT_SITE        154
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         5..36
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         130
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   350 AA;  38374 MW;  C7E6C6CEBE7E31BC CRC64;
     MSSQTVLVTG GAGYIGSHTV LQLLLGGFKA VVVDNLDNSS ETAIHRVKEL AGKFAGNLSF
     HKLDLRDRDA LEKIFSSTKF DSVIHFAGLK AVGESVQKPL LYYDNNLIGT IVLFEVMAAH
     GCKKLVFSSS ATVYGLPKEV PCTEEFPLSA ANPYGRTKLI IEEICRDIYR AEQEWKIILL
     RYFNPVGAHP SGYIGEDPRG IPNNLMPFVQ QVAVGRRPAL TVFGNDYTTS DGTGVRDYIH
     VVDLADGHIA ALRKLNDPKI GCEVYNLGTG KGTSVLEMVK AFEQASGKKI PLVMAGRRPG
     DAEVVYASTN KAERELNWKA KYGIDEMCRD QWNWASKNPY GYGGSEDSSN