ID   GALE_KASSE              Reviewed;          72 AA.
AC   Q90W78; P82448;
DT   25-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 42.
DE   RecName: Full=Galensin;
DE   Flags: Precursor;
OS   Kassina senegalensis (Senegal running frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Microhyloidea; Hyperoliidae; Kassina.
OX   NCBI_TaxID=8415;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Skin;
RA   Reid C.N., Bjourson A.J., Shaw C.;
RT   "Galensin, a novel homodimeric antimicrobial peptide from the defensive
RT   skin secretion of Kassina senegalensis: isolation, structural
RT   characterisation and cloning of precursor cDNA.";
RL   Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PROTEIN SEQUENCE OF 49-71, FUNCTION, TISSUE SPECIFICITY, AND AMIDATION AT
RP   PHE-71.
RC   TISSUE=Skin;
RA   Reid C.N., Bjourson T., Shaw C.;
RL   Submitted (MAR-2000) to UniProtKB.
CC   -!- FUNCTION: Antibacterial activity against the Gram-positive bacterium
CC       M.luteus and the Gram-negative bacterium E.coli. {ECO:0000269|Ref.2}.
CC   -!- SUBUNIT: Homodimer; disulfide-linked.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands. {ECO:0000269|Ref.2}.
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Brevinin subfamily. {ECO:0000305}.
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DR   EMBL; AJ318759; CAC47951.1; -; mRNA.
DR   AlphaFoldDB; Q90W78; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR   Pfam; PF03032; FSAP_sig_propep; 1.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..48
FT                   /evidence="ECO:0000269|Ref.2"
FT                   /id="PRO_0000003459"
FT   PEPTIDE         49..71
FT                   /note="Galensin"
FT                   /id="PRO_0000003460"
FT   MOD_RES         71
FT                   /note="Phenylalanine amide"
FT                   /evidence="ECO:0000269|Ref.2"
FT   DISULFID        49
FT                   /note="Interchain"
SQ   SEQUENCE   72 AA;  8371 MW;  7A626A6A2FE01754 CRC64;
     MLTLKKSMLL LFFLGLVSVS LADDKREDEA EEGEDKRAAE EERNVEKRCY SAAKYPGFQE
     FINRKYKSSR FG