GALF_ECOLI
ID GALF_ECOLI Reviewed; 297 AA.
AC P0AAB6; P71245; P78083; P97192;
DT 13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=UTP--glucose-1-phosphate uridylyltransferase;
DE EC=2.7.7.9;
DE AltName: Full=Alpha-D-glucosyl-1-phosphate uridylyltransferase;
DE AltName: Full=UDP-glucose pyrophosphorylase;
DE Short=UDPGP;
DE AltName: Full=Uridine diphosphoglucose pyrophosphorylase;
GN Name=galF; Synonyms=wcaN; OrderedLocusNames=b2042, JW2027;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP CHARACTERIZATION.
RX PubMed=8971705; DOI=10.1046/j.1365-2958.1996.01531.x;
RA Marolda C.L., Valvano M.A.;
RT "The GalF protein of Escherichia coli is not a UDP-glucose
RT pyrophosphorylase but interacts with the GalU protein possibly to regulate
RT cellular levels of UDP-glucose.";
RL Mol. Microbiol. 22:827-840(1996).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-D-glucose 1-phosphate + H(+) + UTP = diphosphate + UDP-
CC alpha-D-glucose; Xref=Rhea:RHEA:19889, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:46398, ChEBI:CHEBI:58601,
CC ChEBI:CHEBI:58885; EC=2.7.7.9;
CC -!- PATHWAY: Carbohydrate metabolism; nucleotide-sugar metabolism.
CC -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC biosynthesis.
CC -!- SIMILARITY: Belongs to the UDPGP type 2 family. {ECO:0000305}.
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DR EMBL; U38473; AAC77853.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75103.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15896.1; -; Genomic_DNA.
DR PIR; A64970; A64970.
DR RefSeq; NP_416546.1; NC_000913.3.
DR RefSeq; WP_000183060.1; NZ_STEB01000002.1.
DR AlphaFoldDB; P0AAB6; -.
DR SMR; P0AAB6; -.
DR BioGRID; 4259678; 180.
DR DIP; DIP-35871N; -.
DR IntAct; P0AAB6; 17.
DR STRING; 511145.b2042; -.
DR jPOST; P0AAB6; -.
DR PaxDb; P0AAB6; -.
DR PRIDE; P0AAB6; -.
DR EnsemblBacteria; AAC75103; AAC75103; b2042.
DR EnsemblBacteria; BAA15896; BAA15896; BAA15896.
DR GeneID; 66674061; -.
DR GeneID; 946560; -.
DR KEGG; ecj:JW2027; -.
DR KEGG; eco:b2042; -.
DR PATRIC; fig|511145.12.peg.2119; -.
DR EchoBASE; EB3348; -.
DR eggNOG; COG1210; Bacteria.
DR HOGENOM; CLU_029499_1_1_6; -.
DR InParanoid; P0AAB6; -.
DR OMA; MHYVRQG; -.
DR PhylomeDB; P0AAB6; -.
DR BioCyc; EcoCyc:G7093-MON; -.
DR SABIO-RK; P0AAB6; -.
DR UniPathway; UPA00030; -.
DR UniPathway; UPA00215; -.
DR PRO; PR:P0AAB6; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0030234; F:enzyme regulator activity; IEA:InterPro.
DR GO; GO:0003983; F:UTP:glucose-1-phosphate uridylyltransferase activity; IDA:EcoCyc.
DR GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006011; P:UDP-glucose metabolic process; IEA:InterPro.
DR CDD; cd02541; UGPase_prokaryotic; 1.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR005774; GalF.
DR InterPro; IPR005771; GalU_uridylyltTrfase_bac/arc.
DR InterPro; IPR005835; NTP_transferase_dom.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR43197; PTHR43197; 1.
DR Pfam; PF00483; NTP_transferase; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
DR TIGRFAMs; TIGR01105; galF; 1.
PE 1: Evidence at protein level;
KW Lipopolysaccharide biosynthesis; Nucleotidyltransferase;
KW Reference proteome; Transferase.
FT CHAIN 1..297
FT /note="UTP--glucose-1-phosphate uridylyltransferase"
FT /id="PRO_0000201349"
SQ SEQUENCE 297 AA; 32829 MW; 6F85D6BB12C0C5A8 CRC64;
MTNLKAVIPV AGLGMHMLPA TKAIPKEMLP IVDKPMIQYI VDEIVAAGIK EILLVTHASK
NAVENHFDTS YELESLLEQR VKRQLLAEVQ SICPPGVTIM NVRQGEPLGL GHSILCARPA
IGDNPFVVVL PDVVIDDASA DPLRYNLAAM IARFNETGRS QVLAKRMPGD LSEYSVIQTK
EPLDREGKVS RIVEFIEKPD QPQTLDSDIM AVGRYVLSAD IWPELERTQP GAWGRIQLTD
AIAELAKKQS VDAMLMTGDS YDCGKKMGYM QAFVKYGLRN LKEGAKFRKG IEKLLSE
