GALF_SHIFL
ID GALF_SHIFL Reviewed; 297 AA.
AC P37776; Q54161;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2003, sequence version 2.
DT 25-MAY-2022, entry version 133.
DE RecName: Full=UTP--glucose-1-phosphate uridylyltransferase;
DE EC=2.7.7.9;
DE AltName: Full=Alpha-D-glucosyl-1-phosphate uridylyltransferase;
DE AltName: Full=UDP-glucose pyrophosphorylase;
DE Short=UDPGP;
DE AltName: Full=Uridine diphosphoglucose pyrophosphorylase;
GN Name=galF; OrderedLocusNames=SF2105, S2228;
OS Shigella flexneri.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PE577 / Serotype 2a;
RX PubMed=8170390; DOI=10.1111/j.1365-2958.1994.tb00308.x;
RA Macpherson D.F., Manning P.A., Morona R.;
RT "Characterization of the dTDP-rhamnose biosynthetic genes encoded in the
RT rfb locus of Shigella flexneri.";
RL Mol. Microbiol. 11:281-292(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT "Complete genome sequence and comparative genomics of Shigella flexneri
RT serotype 2a strain 2457T.";
RL Infect. Immun. 71:2775-2786(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 169-297.
RC STRAIN=YSH6200 / Serotype 2a;
RX PubMed=8157605; DOI=10.1128/jb.176.8.2362-2373.1994;
RA Rajakumar K., Jost B.H., Sasakawa C., Okada N., Yoshikawa M., Adler B.;
RT "Nucleotide sequence of the rhamnose biosynthetic operon of Shigella
RT flexneri 2a and role of lipopolysaccharide in virulence.";
RL J. Bacteriol. 176:2362-2373(1994).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-D-glucose 1-phosphate + H(+) + UTP = diphosphate + UDP-
CC alpha-D-glucose; Xref=Rhea:RHEA:19889, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:46398, ChEBI:CHEBI:58601,
CC ChEBI:CHEBI:58885; EC=2.7.7.9;
CC -!- PATHWAY: Carbohydrate metabolism; nucleotide-sugar metabolism.
CC -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC biosynthesis.
CC -!- SIMILARITY: Belongs to the UDPGP type 2 family. {ECO:0000305}.
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DR EMBL; X71970; CAA50766.1; -; Genomic_DNA.
DR EMBL; AE005674; AAN43644.2; -; Genomic_DNA.
DR EMBL; AE014073; AAP17473.1; -; Genomic_DNA.
DR EMBL; L14842; AAA53678.1; -; Genomic_DNA.
DR PIR; S41533; S41533.
DR RefSeq; NP_707937.2; NC_004337.2.
DR RefSeq; WP_000183041.1; NZ_WPGW01000076.1.
DR AlphaFoldDB; P37776; -.
DR SMR; P37776; -.
DR STRING; 198214.SF2105; -.
DR EnsemblBacteria; AAN43644; AAN43644; SF2105.
DR EnsemblBacteria; AAP17473; AAP17473; S2228.
DR GeneID; 1025313; -.
DR KEGG; sfl:SF2105; -.
DR KEGG; sfx:S2228; -.
DR PATRIC; fig|198214.7.peg.2513; -.
DR HOGENOM; CLU_029499_1_2_6; -.
DR OMA; MHYVRQG; -.
DR OrthoDB; 1402673at2; -.
DR UniPathway; UPA00030; -.
DR UniPathway; UPA00215; -.
DR Proteomes; UP000001006; Chromosome.
DR Proteomes; UP000002673; Chromosome.
DR GO; GO:0030234; F:enzyme regulator activity; IEA:InterPro.
DR GO; GO:0003983; F:UTP:glucose-1-phosphate uridylyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006011; P:UDP-glucose metabolic process; IEA:InterPro.
DR CDD; cd02541; UGPase_prokaryotic; 1.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR005774; GalF.
DR InterPro; IPR005771; GalU_uridylyltTrfase_bac/arc.
DR InterPro; IPR005835; NTP_transferase_dom.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR43197; PTHR43197; 1.
DR Pfam; PF00483; NTP_transferase; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
DR TIGRFAMs; TIGR01105; galF; 1.
PE 3: Inferred from homology;
KW Lipopolysaccharide biosynthesis; Nucleotidyltransferase;
KW Reference proteome; Transferase.
FT CHAIN 1..297
FT /note="UTP--glucose-1-phosphate uridylyltransferase"
FT /id="PRO_0000201353"
FT CONFLICT 31..32
FT /note="IV -> TA (in Ref. 1; CAA50766)"
FT /evidence="ECO:0000305"
FT CONFLICT 285
FT /note="A -> S (in Ref. 1; CAA50766)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 297 AA; 32841 MW; D693305ECBD9DF8A CRC64;
MTNLKAVIPV AGLGMHMLPA TKAIPKEMLP IVDKPMIQYI VDEIVAAGIK EILLVTHASK
NAVENHFDTS YELESLLEQR VKRQLLAEVQ SICPPGVIIM NVRQGEPLGL GHSILCARPA
IGDNPFVVVL PDVVIDDASA DPLRYNLAAM IARFNETGRS QVLAKRMPGD LSEYSVIQTK
EPLDREGKVS RIVEFIEKPD QPQTLDSDIM AVGRYVLSAD IWPELERTQP GAWGRIQLTD
AIAELAKKQS VDAMLMTGDS YDCGKKMGYM QAFVKYGLRN LKEGAKFRKG IEKLLSE
