GALK1_BOVIN
ID GALK1_BOVIN Reviewed; 392 AA.
AC A6H768; Q1JP66;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 2.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Galactokinase;
DE EC=2.7.1.6 {ECO:0000250|UniProtKB:P51570};
DE AltName: Full=Galactose kinase;
GN Name=GALK1;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Fetal pons;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-290.
RX PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT "Characterization of 954 bovine full-CDS cDNA sequences.";
RL BMC Genomics 6:166-166(2005).
CC -!- FUNCTION: Catalyzes the transfer of a phosphate from ATP to alpha-D-
CC galactose and participates in the first committed step in the
CC catabolism of galactose. {ECO:0000250|UniProtKB:P51570}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-D-galactose + ATP = ADP + alpha-D-galactose 1-phosphate
CC + H(+); Xref=Rhea:RHEA:13553, ChEBI:CHEBI:15378, ChEBI:CHEBI:28061,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:58336, ChEBI:CHEBI:456216; EC=2.7.1.6;
CC Evidence={ECO:0000250|UniProtKB:P51570};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:13554;
CC Evidence={ECO:0000250|UniProtKB:P51570};
CC -!- PATHWAY: Carbohydrate metabolism; galactose metabolism.
CC {ECO:0000250|UniProtKB:P51570}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P51570}.
CC -!- SIMILARITY: Belongs to the GHMP kinase family. GalK subfamily.
CC {ECO:0000305}.
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DR EMBL; BC146132; AAI46133.1; -; mRNA.
DR EMBL; BT025341; ABF57297.1; -; mRNA.
DR EMBL; BT025347; ABF57303.1; -; mRNA.
DR EMBL; BT025488; ABF57444.1; -; mRNA.
DR EMBL; BT026290; ABG81446.1; -; mRNA.
DR RefSeq; NP_001092851.1; NM_001099381.1.
DR AlphaFoldDB; A6H768; -.
DR SMR; A6H768; -.
DR STRING; 9913.ENSBTAP00000019921; -.
DR PaxDb; A6H768; -.
DR PeptideAtlas; A6H768; -.
DR PRIDE; A6H768; -.
DR Ensembl; ENSBTAT00000019921; ENSBTAP00000019921; ENSBTAG00000014964.
DR GeneID; 530855; -.
DR KEGG; bta:530855; -.
DR CTD; 2584; -.
DR VEuPathDB; HostDB:ENSBTAG00000014964; -.
DR VGNC; VGNC:29219; GALK1.
DR eggNOG; KOG0631; Eukaryota.
DR GeneTree; ENSGT00950000183187; -.
DR InParanoid; A6H768; -.
DR OMA; NTHQDYK; -.
DR OrthoDB; 860024at2759; -.
DR UniPathway; UPA00214; -.
DR Proteomes; UP000009136; Chromosome 19.
DR Bgee; ENSBTAG00000014964; Expressed in anterior segment of eyeball and 108 other tissues.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004335; F:galactokinase activity; IBA:GO_Central.
DR GO; GO:0005534; F:galactose binding; IEA:Ensembl.
DR GO; GO:0019402; P:galactitol metabolic process; IEA:Ensembl.
DR GO; GO:0033499; P:galactose catabolic process via UDP-galactose; IEA:Ensembl.
DR GO; GO:0006012; P:galactose metabolic process; IBA:GO_Central.
DR GO; GO:0061623; P:glycolytic process from galactose; IEA:Ensembl.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.30.70.890; -; 1.
DR InterPro; IPR000705; Galactokinase.
DR InterPro; IPR019741; Galactokinase_CS.
DR InterPro; IPR019539; GalKase_N.
DR InterPro; IPR013750; GHMP_kinase_C_dom.
DR InterPro; IPR036554; GHMP_kinase_C_sf.
DR InterPro; IPR006204; GHMP_kinase_N_dom.
DR InterPro; IPR006203; GHMP_knse_ATP-bd_CS.
DR InterPro; IPR006206; Mevalonate/galactokinase.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR PANTHER; PTHR10457:SF6; PTHR10457:SF6; 1.
DR Pfam; PF10509; GalKase_gal_bdg; 1.
DR Pfam; PF08544; GHMP_kinases_C; 1.
DR Pfam; PF00288; GHMP_kinases_N; 1.
DR PIRSF; PIRSF000530; Galactokinase; 1.
DR PRINTS; PR00473; GALCTOKINASE.
DR SUPFAM; SSF54211; SSF54211; 1.
DR SUPFAM; SSF55060; SSF55060; 1.
DR TIGRFAMs; TIGR00131; gal_kin; 1.
DR PROSITE; PS00106; GALACTOKINASE; 1.
DR PROSITE; PS00627; GHMP_KINASES_ATP; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Carbohydrate metabolism; Galactose metabolism; Kinase;
KW Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase.
FT CHAIN 1..392
FT /note="Galactokinase"
FT /id="PRO_0000320045"
FT ACT_SITE 186
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 43..46
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 134..144
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 183..186
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 236
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT SITE 37
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000250"
FT MOD_RES 230
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P51570"
FT CONFLICT 137
FT /note="G -> D (in Ref. 1; AAI46133)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 392 AA; 42227 MW; CDAE6E34871EA9D4 CRC64;
MAASEQPQAG ELLAKARRAF LEEFGAEPEL AVSAPGRVNL IGEHTDYNRG LVLPMALELV
TVLVGSPRVD GLVSLLTTSE DADEPRRLQF PLPTSQRPLE PGTPHWANYV KGVIQHYPAA
PLPGFSAVVV SSVPLGGGLS SSASLEVATY TFLQQLCPDS GTIAARAQVC QRAEHSFAGV
PCGIMDQLIA LLGQRGHALL IDCRSLETSL VPLSDPKLAV LITNSNVRHS LGSSEYPLRR
RQCEEVARAL GKESLREVQL EELEAGRDLM STEAFRRARH VVGEIQRTAQ AAAALRRGDY
RAFGRLMVES HHSLRDDYEV SCPELDQLVE AALSAPGVYG SRMTGGGFGG CTVTLLEASA
APRVMQHIQE QYHGTATFYL SQAADGAKVL HF
