GALK2_MOUSE
ID GALK2_MOUSE Reviewed; 458 AA.
AC Q68FH4;
DT 13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=N-acetylgalactosamine kinase;
DE EC=2.7.1.157;
DE AltName: Full=GalNAc kinase;
DE AltName: Full=Galactokinase 2;
GN Name=Galk2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP PROTEIN SEQUENCE OF 313-327, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=OF1; TISSUE=Hippocampus;
RA Lubec G., Sunyer B., Chen W.-Q.;
RL Submitted (JAN-2009) to UniProtKB.
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Liver, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Acts on GalNAc. Also acts as a galactokinase when galactose
CC is present at high concentrations (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + N-acetyl-alpha-D-galactosamine = ADP + H(+) + N-acetyl-
CC alpha-D-galactosamine 1-phosphate; Xref=Rhea:RHEA:12617,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:40356,
CC ChEBI:CHEBI:61970, ChEBI:CHEBI:456216; EC=2.7.1.157;
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the GHMP kinase family. GalK subfamily.
CC {ECO:0000305}.
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DR EMBL; BC079843; AAH79843.1; -; mRNA.
DR CCDS; CCDS71134.1; -.
DR RefSeq; NP_001277931.1; NM_001291002.1.
DR AlphaFoldDB; Q68FH4; -.
DR SMR; Q68FH4; -.
DR STRING; 10090.ENSMUSP00000028636; -.
DR iPTMnet; Q68FH4; -.
DR PhosphoSitePlus; Q68FH4; -.
DR EPD; Q68FH4; -.
DR jPOST; Q68FH4; -.
DR MaxQB; Q68FH4; -.
DR PaxDb; Q68FH4; -.
DR PRIDE; Q68FH4; -.
DR ProteomicsDB; 267417; -.
DR Antibodypedia; 24645; 268 antibodies from 26 providers.
DR DNASU; 69976; -.
DR Ensembl; ENSMUST00000094604; ENSMUSP00000092186; ENSMUSG00000027207.
DR GeneID; 69976; -.
DR KEGG; mmu:69976; -.
DR UCSC; uc008mdg.2; mouse.
DR CTD; 2585; -.
DR MGI; MGI:1917226; Galk2.
DR VEuPathDB; HostDB:ENSMUSG00000027207; -.
DR eggNOG; KOG0631; Eukaryota.
DR GeneTree; ENSGT00950000183187; -.
DR InParanoid; Q68FH4; -.
DR OrthoDB; 860024at2759; -.
DR PhylomeDB; Q68FH4; -.
DR BioGRID-ORCS; 69976; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Galk2; mouse.
DR PRO; PR:Q68FH4; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q68FH4; protein.
DR Bgee; ENSMUSG00000027207; Expressed in right kidney and 253 other tissues.
DR ExpressionAtlas; Q68FH4; baseline and differential.
DR Genevisible; Q68FH4; MM.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004335; F:galactokinase activity; ISO:MGI.
DR GO; GO:0033858; F:N-acetylgalactosamine kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0006012; P:galactose metabolic process; IBA:GO_Central.
DR Gene3D; 3.30.230.10; -; 1.
DR InterPro; IPR000705; Galactokinase.
DR InterPro; IPR019741; Galactokinase_CS.
DR InterPro; IPR019539; GalKase_N.
DR InterPro; IPR013750; GHMP_kinase_C_dom.
DR InterPro; IPR036554; GHMP_kinase_C_sf.
DR InterPro; IPR006204; GHMP_kinase_N_dom.
DR InterPro; IPR006203; GHMP_knse_ATP-bd_CS.
DR InterPro; IPR006206; Mevalonate/galactokinase.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR Pfam; PF10509; GalKase_gal_bdg; 1.
DR Pfam; PF08544; GHMP_kinases_C; 1.
DR Pfam; PF00288; GHMP_kinases_N; 1.
DR PIRSF; PIRSF000530; Galactokinase; 1.
DR PRINTS; PR00473; GALCTOKINASE.
DR SUPFAM; SSF54211; SSF54211; 1.
DR SUPFAM; SSF55060; SSF55060; 1.
DR TIGRFAMs; TIGR00131; gal_kin; 1.
DR PROSITE; PS00106; GALACTOKINASE; 1.
DR PROSITE; PS00627; GHMP_KINASES_ATP; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Direct protein sequencing; Kinase; Nucleotide-binding;
KW Reference proteome; Transferase.
FT CHAIN 1..458
FT /note="N-acetylgalactosamine kinase"
FT /id="PRO_0000184648"
FT ACT_SITE 190
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 49..52
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 49
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 139..149
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 187..190
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT SITE 43
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000250"
SQ SEQUENCE 458 AA; 50503 MW; 624A5B9DE149C126 CRC64;
MAAEDPATRR VQVAEHPRLL KLKEMFNSKF GSTPKFYVRA PGRVNIIGEH IDYCGYSVIP
MAVEQDMLIA VEPVKTHTLQ LANTDPLYPD FSTTANNICI DKTKPLWHNY FLCGFKGIQE
HFGLSKLPGM NCLVDGNIPP SSGLSSSSAL VCCAGLVTLT VLGLRLSKVE LAEICAKSER
YIGTEGGGMD QSISFLAEEG TAKLIEFSPL RATNVKLPSG AVFVIANSCM EMNKAATSHF
NVRVMECRLA AKVLAKHKGL QWDNVLRLEE VQSKLGISLE EMLLVTEDAL HPEPYSREEI
CRCLGISLER LRTQILTPNT QDELTFKLYQ RAKHVYSEAA RVLQFKQVCE DAPDNAVQLL
GELMNQSHRS CRDMYECSCP ELDQLVDICR KFGAKGSRLT GAGWGGCTVS LVPADMLSSF
LASVHEAYYQ GNTSRLAQEK HSLFATKPGG GALVFREV
