ID   GALK2_RAT               Reviewed;         458 AA.
AC   Q5XIG6;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=N-acetylgalactosamine kinase;
DE            EC=2.7.1.157;
DE   AltName: Full=GalNAc kinase;
DE   AltName: Full=Galactokinase 2;
GN   Name=Galk2;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Heart;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Acts on GalNAc. Also acts as a galactokinase when galactose
CC       is present at high concentrations (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + N-acetyl-alpha-D-galactosamine = ADP + H(+) + N-acetyl-
CC         alpha-D-galactosamine 1-phosphate; Xref=Rhea:RHEA:12617,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:40356,
CC         ChEBI:CHEBI:61970, ChEBI:CHEBI:456216; EC=2.7.1.157;
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the GHMP kinase family. GalK subfamily.
CC       {ECO:0000305}.
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DR   EMBL; BC083716; AAH83716.1; -; mRNA.
DR   RefSeq; NP_001013941.1; NM_001013919.1.
DR   AlphaFoldDB; Q5XIG6; -.
DR   SMR; Q5XIG6; -.
DR   STRING; 10116.ENSRNOP00000012447; -.
DR   PaxDb; Q5XIG6; -.
DR   PRIDE; Q5XIG6; -.
DR   Ensembl; ENSRNOT00000012447; ENSRNOP00000012447; ENSRNOG00000009289.
DR   GeneID; 296117; -.
DR   KEGG; rno:296117; -.
DR   UCSC; RGD:1308691; rat.
DR   CTD; 2585; -.
DR   RGD; 1308691; Galk2.
DR   eggNOG; KOG0631; Eukaryota.
DR   GeneTree; ENSGT00950000183187; -.
DR   InParanoid; Q5XIG6; -.
DR   OMA; PVCYNLR; -.
DR   OrthoDB; 860024at2759; -.
DR   PhylomeDB; Q5XIG6; -.
DR   TreeFam; TF324235; -.
DR   PRO; PR:Q5XIG6; -.
DR   Proteomes; UP000002494; Chromosome 3.
DR   Bgee; ENSRNOG00000009289; Expressed in adult mammalian kidney and 18 other tissues.
DR   ExpressionAtlas; Q5XIG6; baseline and differential.
DR   Genevisible; Q5XIG6; RN.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004335; F:galactokinase activity; ISO:RGD.
DR   GO; GO:0033858; F:N-acetylgalactosamine kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006012; P:galactose metabolic process; IBA:GO_Central.
DR   Gene3D; 3.30.230.10; -; 1.
DR   InterPro; IPR000705; Galactokinase.
DR   InterPro; IPR019741; Galactokinase_CS.
DR   InterPro; IPR019539; GalKase_N.
DR   InterPro; IPR013750; GHMP_kinase_C_dom.
DR   InterPro; IPR036554; GHMP_kinase_C_sf.
DR   InterPro; IPR006204; GHMP_kinase_N_dom.
DR   InterPro; IPR006203; GHMP_knse_ATP-bd_CS.
DR   InterPro; IPR006206; Mevalonate/galactokinase.
DR   InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR   InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR   Pfam; PF10509; GalKase_gal_bdg; 1.
DR   Pfam; PF08544; GHMP_kinases_C; 1.
DR   Pfam; PF00288; GHMP_kinases_N; 1.
DR   PIRSF; PIRSF000530; Galactokinase; 1.
DR   PRINTS; PR00473; GALCTOKINASE.
DR   SUPFAM; SSF54211; SSF54211; 1.
DR   SUPFAM; SSF55060; SSF55060; 1.
DR   TIGRFAMs; TIGR00131; gal_kin; 1.
DR   PROSITE; PS00106; GALACTOKINASE; 1.
DR   PROSITE; PS00627; GHMP_KINASES_ATP; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Kinase; Nucleotide-binding; Reference proteome; Transferase.
FT   CHAIN           1..458
FT                   /note="N-acetylgalactosamine kinase"
FT                   /id="PRO_0000184650"
FT   ACT_SITE        190
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         49..52
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         139..149
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   BINDING         187..190
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            43
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   458 AA;  50198 MW;  4F582ACF66DB3231 CRC64;
     MAAEDPATRR VQVAEHPRLL KLKEMFNSKF GSTPKFYVRA PGRVNIIGEH IDYCGYSVLP
     MAVEQDMLIA VGPVKTQTLQ LANTDPLYPD FSTTANNICI DKTQPLWHNY FLCGFKGIQE
     HFGLTQLPGM NCLVDGNIPP SSGLSSSSAL VCCAGLVTLT VLGMRLSKVE LAEICAKSER
     YIGTEGGGMD QSISFLAEEG TAKLIEFSPL RATDVKLPSG AVFVIANSCV EMNKAATSHF
     NVRVMECRLA AKVLAKHKGL QWDKVLRLEE VQSELGISLE EMLLVTEDAL HAEPYSREEI
     CKCLGISLEE LRTQILSPNT QGELTFKLYQ RAKHVYSEAA RVLQFKQVCE AAPDNAVQLL
     GELMNQSHRS CRDMYECSCP ELDQLVDICR KFGAKGSRLT GAGWGGCTVS LVPADTLSSF
     LASVHEAYYQ GNMSRLAREK HSLFATKPGG GALVFLEA