ID   GALK_DICDI              Reviewed;         501 AA.
AC   Q54DN6;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Galactokinase;
DE            EC=2.7.1.6;
DE   AltName: Full=Galactose kinase;
GN   Name=galK; ORFNames=DDB_G0292112;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-D-galactose + ATP = ADP + alpha-D-galactose 1-phosphate
CC         + H(+); Xref=Rhea:RHEA:13553, ChEBI:CHEBI:15378, ChEBI:CHEBI:28061,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:58336, ChEBI:CHEBI:456216; EC=2.7.1.6;
CC   -!- PATHWAY: Carbohydrate metabolism; galactose metabolism.
CC   -!- SIMILARITY: Belongs to the GHMP kinase family. GalK subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000187; EAL61412.1; -; Genomic_DNA.
DR   RefSeq; XP_629836.1; XM_629834.1.
DR   AlphaFoldDB; Q54DN6; -.
DR   SMR; Q54DN6; -.
DR   STRING; 44689.DDB0231667; -.
DR   PaxDb; Q54DN6; -.
DR   EnsemblProtists; EAL61412; EAL61412; DDB_G0292112.
DR   GeneID; 8628517; -.
DR   KEGG; ddi:DDB_G0292112; -.
DR   dictyBase; DDB_G0292112; galK.
DR   eggNOG; KOG0631; Eukaryota.
DR   HOGENOM; CLU_017814_6_2_1; -.
DR   InParanoid; Q54DN6; -.
DR   OMA; PVCYNLR; -.
DR   PhylomeDB; Q54DN6; -.
DR   UniPathway; UPA00214; -.
DR   PRO; PR:Q54DN6; -.
DR   Proteomes; UP000002195; Chromosome 6.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004335; F:galactokinase activity; ISS:dictyBase.
DR   GO; GO:0019388; P:galactose catabolic process; ISS:dictyBase.
DR   GO; GO:0006012; P:galactose metabolic process; IBA:GO_Central.
DR   Gene3D; 3.30.230.10; -; 1.
DR   InterPro; IPR000705; Galactokinase.
DR   InterPro; IPR019741; Galactokinase_CS.
DR   InterPro; IPR019539; GalKase_N.
DR   InterPro; IPR013750; GHMP_kinase_C_dom.
DR   InterPro; IPR036554; GHMP_kinase_C_sf.
DR   InterPro; IPR006204; GHMP_kinase_N_dom.
DR   InterPro; IPR006206; Mevalonate/galactokinase.
DR   InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR   InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR   Pfam; PF10509; GalKase_gal_bdg; 1.
DR   Pfam; PF08544; GHMP_kinases_C; 1.
DR   Pfam; PF00288; GHMP_kinases_N; 1.
DR   PIRSF; PIRSF000530; Galactokinase; 1.
DR   PRINTS; PR00473; GALCTOKINASE.
DR   SUPFAM; SSF54211; SSF54211; 1.
DR   SUPFAM; SSF55060; SSF55060; 1.
DR   TIGRFAMs; TIGR00131; gal_kin; 1.
DR   PROSITE; PS00106; GALACTOKINASE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Carbohydrate metabolism; Galactose metabolism; Kinase;
KW   Nucleotide-binding; Reference proteome; Transferase.
FT   CHAIN           1..501
FT                   /note="Galactokinase"
FT                   /id="PRO_0000320046"
FT   ACT_SITE        207
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         156..166
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   SITE            54
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   501 AA;  56128 MW;  39C16C0255483245 CRC64;
     MDSFNPALPS IVESLDDIYK NFENNKKRYE ELNETFSKIY NGDKPIFYFR APGRVNLIGE
     HVDYSGYCVL PFALEQDTIV AVSFNKLNND IINIHNCNEK YTPKSIDVSG GGDIEIDMKR
     HHWTNYVLAA WKGVSQAMEK GGKLKSVNLL YSGNVPMGAG VSSSSALVCV STLAISYCNN
     LILNKEELAQ LSIKSERYVG VESGGMDQSI SFLAEQNTAK LIEFHPSLKT FDVQLPKGVS
     FVICNSLVDS LKVVTGATNY NLRVVECRLA AVLLAFHCGL SWEKVRRLRD VQYQGNFDLP
     QLIQLTEQHL SEKQTYTREE VATILDISVE QLVKTYFPSG ITVQSEHFEL YKRARHVFTE
     TQRVYKFSEI CKQQSNFNNN NNNNNNNSSN NTNIIQELGK LMNESHESCS KLFECSCSEL
     DSLTKICREN GALGSRLTGA GWGGCVISLV PNSKVDSFLD AIDTHYYSKF VNPEKLKNIE
     KSSYSFFTTP CKGACIVSST V