GALL1_DROME
ID GALL1_DROME Reviewed; 218 AA.
AC Q9V968;
DT 02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=MIP18 family protein galla-1 {ECO:0000303|PubMed:25065591};
GN Name=galla-1 {ECO:0000303|PubMed:25065591,
GN ECO:0000312|FlyBase:FBgn0034543};
GN ORFNames=CG30152 {ECO:0000312|FlyBase:FBgn0034543};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Larva, and Pupae;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14 AND SER-65, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
RN [5]
RP FUNCTION, INTERACTION WITH THE CGX COMPLEX, SUBCELLULAR LOCATION,
RP DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=25065591; DOI=10.1038/onc.2014.202;
RA Yeom E., Hong S.T., Choi K.W.;
RT "Crumbs interacts with Xpd for nuclear division control in Drosophila.";
RL Oncogene 34:2777-2789(2015).
CC -!- FUNCTION: Component of the crb-galla-Xpd (CGX) complex which is
CC essential for proper mitotic chromosome segregation in early embryos.
CC The CGX complex is also required for cell proliferation in developing
CC wing disks. In the CGX complex, acts with crb to recruit Xpd thus
CC forming the functional complex. {ECO:0000269|PubMed:25065591}.
CC -!- SUBUNIT: Component of the CGX complex composed of crb, galla (galla-1
CC or galla-2) and Xpd. Interacts with crb (via intracellular domain). Is
CC not able to interact with Xpd in the absence of crb.
CC {ECO:0000269|PubMed:25065591}.
CC -!- SUBCELLULAR LOCATION: Apical cell membrane
CC {ECO:0000269|PubMed:25065591}. Cytoplasm, cytoskeleton, spindle
CC {ECO:0000269|PubMed:25065591}. Note=Expressed in a punctate pattern in
CC spindle microtubules during metaphase. {ECO:0000269|PubMed:25065591}.
CC -!- DEVELOPMENTAL STAGE: Expressed at low levels during larval stages, and
CC at higher levels in pupae and adults (at protein level). Ubiquitously
CC expressed in the wing disk (at protein level).
CC {ECO:0000269|PubMed:25065591}.
CC -!- DISRUPTION PHENOTYPE: Mutant flies show temperature-sensitive
CC lethality. Prior to cellularization many embryos display signs of
CC incomplete chromosome segregation during mitotic divisions likely due
CC to defective organization of spindle microtubules.
CC {ECO:0000269|PubMed:25065591}.
CC -!- MISCELLANEOUS: The name 'galla' means splitting in Korean and is
CC derived from its role in chromosome segregation.
CC {ECO:0000303|PubMed:25065591}.
CC -!- SIMILARITY: Belongs to the MIP18 family. {ECO:0000305}.
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DR EMBL; AE013599; AAF57428.1; -; Genomic_DNA.
DR EMBL; AY118612; AAM49981.1; -; mRNA.
DR RefSeq; NP_001286642.1; NM_001299713.1.
DR RefSeq; NP_001286643.1; NM_001299714.1.
DR RefSeq; NP_611509.1; NM_137665.2.
DR AlphaFoldDB; Q9V968; -.
DR SMR; Q9V968; -.
DR BioGRID; 73054; 4.
DR IntAct; Q9V968; 1.
DR STRING; 7227.FBpp0085549; -.
DR iPTMnet; Q9V968; -.
DR PaxDb; Q9V968; -.
DR PRIDE; Q9V968; -.
DR DNASU; 246485; -.
DR EnsemblMetazoa; FBtr0086236; FBpp0085549; FBgn0034543.
DR EnsemblMetazoa; FBtr0342718; FBpp0309592; FBgn0034543.
DR EnsemblMetazoa; FBtr0345353; FBpp0311508; FBgn0034543.
DR GeneID; 246485; -.
DR KEGG; dme:Dmel_CG30152; -.
DR UCSC; CG30152-RA; d. melanogaster.
DR CTD; 246485; -.
DR FlyBase; FBgn0034543; galla-1.
DR VEuPathDB; VectorBase:FBgn0034543; -.
DR eggNOG; KOG3381; Eukaryota.
DR GeneTree; ENSGT00390000017697; -.
DR InParanoid; Q9V968; -.
DR OrthoDB; 1408004at2759; -.
DR PhylomeDB; Q9V968; -.
DR SignaLink; Q9V968; -.
DR BioGRID-ORCS; 246485; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 246485; -.
DR PRO; PR:Q9V968; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0034543; Expressed in adult midgut (Drosophila) and 27 other tissues.
DR ExpressionAtlas; Q9V968; baseline and differential.
DR Genevisible; Q9V968; DM.
DR GO; GO:0016324; C:apical plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005876; C:spindle microtubule; IDA:FlyBase.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0098813; P:nuclear chromosome segregation; IMP:FlyBase.
DR GO; GO:0106035; P:protein maturation by [4Fe-4S] cluster transfer; IEA:InterPro.
DR Gene3D; 3.30.300.130; -; 1.
DR InterPro; IPR034904; FSCA_dom_sf.
DR InterPro; IPR039796; MIP18.
DR InterPro; IPR002744; MIP18-like.
DR PANTHER; PTHR12377; PTHR12377; 1.
DR Pfam; PF01883; FeS_assembly_P; 1.
DR SUPFAM; SSF117916; SSF117916; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Cell membrane; Chromosome partition; Cytoplasm;
KW Cytoskeleton; Membrane; Mitosis; Phosphoprotein; Reference proteome.
FT CHAIN 1..218
FT /note="MIP18 family protein galla-1"
FT /id="PRO_0000212695"
FT REGION 1..59
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 10..59
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 14
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 65
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 218 AA; 23806 MW; E9A354BC59CBA11B CRC64;
MLSYIKRKLS ESDSGVSSVA TVTSSCGGDS GRAGGTGSSE SGTGSSSASI SGRSQNADEL
VRKTSQMSMD DEAIAFGEDA LLHELGYKNQ TELQETIYDL LRGIRDPEKP CTLEDLNVVY
EDGIFVMPPT RSNVSVVRIE FNPTVPHCSL ATLIGLCIRV KVERGLPHNI KLDIYIKKGA
HQTEEEINKQ INDKERIAAA MENPNLRDLV ENCIKDEE
