GALM_BACSU
ID GALM_BACSU Reviewed; 325 AA.
AC P39840;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-1998, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Aldose 1-epimerase;
DE EC=5.1.3.3;
DE AltName: Full=Galactose mutarotase;
GN Name=galM; Synonyms=yoxA; OrderedLocusNames=BSU18360;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-5, CATALYTIC
RP ACTIVITY, INDUCTION, AND DEVELOPMENTAL STAGE.
RC STRAIN=168;
RX PubMed=19758330; DOI=10.1111/j.1574-6968.2009.01761.x;
RA Duez C., Zervosen A., Teller N., Melkonian R., Banzubaze E., Bouillenne F.,
RA Luxen A., Frere J.-M.;
RT "Characterization of the proteins encoded by the Bacillus subtilis yoxA-
RT dacC operon.";
RL FEMS Microbiol. Lett. 300:42-47(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 284-325.
RC STRAIN=168;
RX PubMed=7711903; DOI=10.1099/13500872-141-3-645;
RA Tognoni A., Franchi E., Magistrelli C., Colombo E., Cosmina P., Grandi G.;
RT "A putative new peptide synthase operon in Bacillus subtilis: partial
RT characterization.";
RL Microbiology 141:645-648(1995).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-D-glucose = beta-D-glucose; Xref=Rhea:RHEA:10264,
CC ChEBI:CHEBI:15903, ChEBI:CHEBI:17925; EC=5.1.3.3;
CC Evidence={ECO:0000269|PubMed:19758330};
CC -!- PATHWAY: Carbohydrate metabolism; hexose metabolism.
CC -!- DEVELOPMENTAL STAGE: Expressed at the end of the exponential growth
CC phase. {ECO:0000269|PubMed:19758330}.
CC -!- INDUCTION: By nitrogen or tryptophan starvation.
CC {ECO:0000269|PubMed:19758330}.
CC -!- SIMILARITY: Belongs to the aldose epimerase family. {ECO:0000305}.
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DR EMBL; AL009126; CAB13719.1; -; Genomic_DNA.
DR EMBL; Z34883; CAA84365.1; -; Genomic_DNA.
DR PIR; A69930; A69930.
DR RefSeq; NP_389718.1; NC_000964.3.
DR RefSeq; WP_004399304.1; NZ_JNCM01000036.1.
DR PDB; 3MWX; X-ray; 1.45 A; A/B=1-325.
DR PDB; 4BZE; X-ray; 2.00 A; A/B=1-325.
DR PDB; 4BZF; X-ray; 1.90 A; A/B=2-325.
DR PDB; 4BZG; X-ray; 2.13 A; A/B=2-325.
DR PDB; 4BZH; X-ray; 2.60 A; A/B=2-325.
DR PDBsum; 3MWX; -.
DR PDBsum; 4BZE; -.
DR PDBsum; 4BZF; -.
DR PDBsum; 4BZG; -.
DR PDBsum; 4BZH; -.
DR AlphaFoldDB; P39840; -.
DR SMR; P39840; -.
DR STRING; 224308.BSU18360; -.
DR PaxDb; P39840; -.
DR PRIDE; P39840; -.
DR DNASU; 938046; -.
DR EnsemblBacteria; CAB13719; CAB13719; BSU_18360.
DR GeneID; 938046; -.
DR KEGG; bsu:BSU18360; -.
DR PATRIC; fig|224308.179.peg.2003; -.
DR eggNOG; COG2017; Bacteria.
DR InParanoid; P39840; -.
DR OMA; IHGSAWQ; -.
DR PhylomeDB; P39840; -.
DR BioCyc; BSUB:BSU18360-MON; -.
DR UniPathway; UPA00242; -.
DR EvolutionaryTrace; P39840; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0004034; F:aldose 1-epimerase activity; IBA:GO_Central.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0033499; P:galactose catabolic process via UDP-galactose; IBA:GO_Central.
DR GO; GO:0006006; P:glucose metabolic process; IBA:GO_Central.
DR Gene3D; 2.70.98.10; -; 1.
DR InterPro; IPR008183; Aldose_1/G6P_1-epimerase.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR014718; GH-type_carb-bd.
DR Pfam; PF01263; Aldose_epim; 1.
DR SUPFAM; SSF74650; SSF74650; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Carbohydrate metabolism; Direct protein sequencing;
KW Isomerase; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:19758330"
FT CHAIN 2..325
FT /note="Aldose 1-epimerase"
FT /id="PRO_0000049666"
FT ACT_SITE 177
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 283
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 73..74
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 230
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT STRAND 3..10
FT /evidence="ECO:0007829|PDB:3MWX"
FT STRAND 13..28
FT /evidence="ECO:0007829|PDB:3MWX"
FT TURN 29..33
FT /evidence="ECO:0007829|PDB:3MWX"
FT STRAND 34..40
FT /evidence="ECO:0007829|PDB:3MWX"
FT TURN 41..44
FT /evidence="ECO:0007829|PDB:3MWX"
FT HELIX 54..59
FT /evidence="ECO:0007829|PDB:3MWX"
FT STRAND 63..65
FT /evidence="ECO:0007829|PDB:3MWX"
FT STRAND 68..71
FT /evidence="ECO:0007829|PDB:3MWX"
FT STRAND 73..76
FT /evidence="ECO:0007829|PDB:3MWX"
FT STRAND 79..82
FT /evidence="ECO:0007829|PDB:3MWX"
FT STRAND 85..88
FT /evidence="ECO:0007829|PDB:3MWX"
FT TURN 94..97
FT /evidence="ECO:0007829|PDB:3MWX"
FT STRAND 98..103
FT /evidence="ECO:0007829|PDB:3MWX"
FT STRAND 110..119
FT /evidence="ECO:0007829|PDB:3MWX"
FT STRAND 121..128
FT /evidence="ECO:0007829|PDB:3MWX"
FT HELIX 129..131
FT /evidence="ECO:0007829|PDB:3MWX"
FT HELIX 133..138
FT /evidence="ECO:0007829|PDB:3MWX"
FT STRAND 143..152
FT /evidence="ECO:0007829|PDB:3MWX"
FT STRAND 155..164
FT /evidence="ECO:0007829|PDB:3MWX"
FT STRAND 166..168
FT /evidence="ECO:0007829|PDB:3MWX"
FT STRAND 170..172
FT /evidence="ECO:0007829|PDB:3MWX"
FT TURN 184..186
FT /evidence="ECO:0007829|PDB:3MWX"
FT STRAND 187..199
FT /evidence="ECO:0007829|PDB:3MWX"
FT STRAND 205..211
FT /evidence="ECO:0007829|PDB:3MWX"
FT HELIX 215..220
FT /evidence="ECO:0007829|PDB:3MWX"
FT STRAND 230..236
FT /evidence="ECO:0007829|PDB:3MWX"
FT HELIX 237..240
FT /evidence="ECO:0007829|PDB:3MWX"
FT STRAND 245..250
FT /evidence="ECO:0007829|PDB:3MWX"
FT TURN 251..254
FT /evidence="ECO:0007829|PDB:3MWX"
FT STRAND 255..261
FT /evidence="ECO:0007829|PDB:3MWX"
FT STRAND 267..271
FT /evidence="ECO:0007829|PDB:3MWX"
FT STRAND 277..287
FT /evidence="ECO:0007829|PDB:3MWX"
FT HELIX 291..293
FT /evidence="ECO:0007829|PDB:3MWX"
FT HELIX 298..301
FT /evidence="ECO:0007829|PDB:3MWX"
FT STRAND 304..306
FT /evidence="ECO:0007829|PDB:3MWX"
FT STRAND 311..322
FT /evidence="ECO:0007829|PDB:3MWX"
SQ SEQUENCE 325 AA; 37228 MW; CD80CB777AF4B829 CRC64;
MANFIEKITY LGTPAIKAGN EHLEMIVVPE WGSNVISLVD KTTNVQLLRE PETAESFHDT
PTLYGIPILF PPNRISDGTF SFRGRTYHFD INEKDKHNHL HGFLYHEKWN VVTTKQTDEG
VIVETEIDLS ELPHVQKQFP HHAVVRMTYT IKENTLFKHA TVMNKGKEAF PWGIGYHTTF
IFPAESSLFS LTADQQWELD ERLLPTGKLM DVPYKEALHE GMDLRHKQLD DVFLSSYQKR
GGENQAVIYH QHAHISIIYK ADEQFKHWVV YNADGKQGYL CPEPYTWVTN AVNLDLPSSL
TGLQVLEPGE ETTAKSSITI ELNHQ
