ALGF_PSEAE
ID ALGF_PSEAE Reviewed; 216 AA.
AC Q06062; Q51394;
DT 25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 25-JUL-2003, sequence version 2.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Alginate biosynthesis protein AlgF;
DE Flags: Precursor;
GN Name=algF; OrderedLocusNames=PA3550;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7934909; DOI=10.1111/j.1365-2958.1993.tb01232.x;
RA Shinabarger D., May T.B., Boyd A., Ghosh M., Chakrabarty A.M.;
RT "Nucleotide sequence and expression of the Pseudomonas aeruginosa algF gene
RT controlling acetylation of alginate.";
RL Mol. Microbiol. 9:1027-1035(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=FRD1;
RX PubMed=8636017; DOI=10.1128/jb.178.8.2186-2195.1996;
RA Franklin M.J., Ohman D.E.;
RT "Identification of algI and algJ in the Pseudomonas aeruginosa alginate
RT biosynthetic gene cluster which are required for alginate O acetylation.";
RL J. Bacteriol. 178:2186-2195(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [4]
RP SUBCELLULAR LOCATION.
RC STRAIN=FRD1;
RX PubMed=12003941; DOI=10.1128/jb.184.11.3000-3007.2002;
RA Franklin M.J., Ohman D.E.;
RT "Mutant analysis and cellular localization of the AlgI, AlgJ, and AlgF
RT proteins required for O acetylation of alginate in Pseudomonas
RT aeruginosa.";
RL J. Bacteriol. 184:3000-3007(2002).
CC -!- FUNCTION: Together with AlgI and AlgJ, forms an inner membrane complex
CC which probably interacts with the alginate polymerization-transport
CC complex and adds acetyl groups at the O-2 and O-3 positions of
CC mannuronate residues. Acetylation of alginate is important for the
CC architecture of biofilms and increases resistance to opsonic killing in
CC the host.
CC -!- PATHWAY: Glycan biosynthesis; alginate biosynthesis.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000269|PubMed:12003941}.
CC -!- SIMILARITY: Belongs to the AlgF family. {ECO:0000305}.
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DR EMBL; L13026; AAD15231.1; -; Genomic_DNA.
DR EMBL; U50202; AAB09783.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG06938.1; -; Genomic_DNA.
DR PIR; A83201; A83201.
DR PIR; S37351; S37351.
DR RefSeq; NP_252240.1; NC_002516.2.
DR RefSeq; WP_003092112.1; NZ_QZGE01000001.1.
DR PDB; 6CZT; NMR; -; A=30-216.
DR PDB; 6D10; Other; -; A=30-216.
DR PDBsum; 6CZT; -.
DR PDBsum; 6D10; -.
DR AlphaFoldDB; Q06062; -.
DR SMR; Q06062; -.
DR STRING; 287.DR97_4392; -.
DR PaxDb; Q06062; -.
DR DNASU; 878717; -.
DR EnsemblBacteria; AAG06938; AAG06938; PA3550.
DR GeneID; 878717; -.
DR KEGG; pae:PA3550; -.
DR PATRIC; fig|208964.12.peg.3715; -.
DR PseudoCAP; PA3550; -.
DR HOGENOM; CLU_112428_0_0_6; -.
DR OMA; SSDFSFM; -.
DR PhylomeDB; Q06062; -.
DR BioCyc; MetaCyc:MON-19200; -.
DR BioCyc; PAER208964:G1FZ6-3618-MON; -.
DR UniPathway; UPA00286; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0051979; P:alginic acid acetylation; IDA:PseudoCAP.
DR GO; GO:0042121; P:alginic acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR035422; AlgF.
DR Pfam; PF11182; AlgF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alginate biosynthesis; Periplasm; Reference proteome; Signal.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..216
FT /note="Alginate biosynthesis protein AlgF"
FT /id="PRO_0000001115"
FT CONFLICT 11
FT /note="T -> N (in Ref. 1; AAD15231)"
FT /evidence="ECO:0000305"
FT STRAND 43..49
FT /evidence="ECO:0007829|PDB:6CZT"
FT STRAND 51..53
FT /evidence="ECO:0007829|PDB:6CZT"
FT STRAND 57..59
FT /evidence="ECO:0007829|PDB:6CZT"
FT STRAND 72..79
FT /evidence="ECO:0007829|PDB:6CZT"
FT STRAND 85..87
FT /evidence="ECO:0007829|PDB:6CZT"
FT STRAND 90..92
FT /evidence="ECO:0007829|PDB:6CZT"
FT STRAND 100..106
FT /evidence="ECO:0007829|PDB:6CZT"
FT STRAND 113..117
FT /evidence="ECO:0007829|PDB:6CZT"
SQ SEQUENCE 216 AA; 22834 MW; 20FE4D4FDEE9AF6B CRC64;
MNPMTRRHTW TRLACALSLG VAAFAAQADE GALYGPQAPK GSAFVRAYNA GNSELDVSVG
STSLNDVAPL GSSDFKFLPP GSYTAQVGQQ SLPVKLDPDS YYTLVSQPGG KPQLVAEPPF
KNKQKALVRV QNLSGSKLTL KTADGKTDVV KDVGPQSHGD REINPVKVNL ALFDGSKKVS
DLKPVTLARG EVVCLYVTGS GGKLAPVWVK RPVKAD
