ALGF_PSEFL
ID ALGF_PSEFL Reviewed; 217 AA.
AC P59791;
DT 25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 25-JUL-2003, sequence version 1.
DT 25-MAY-2022, entry version 47.
DE RecName: Full=Alginate biosynthesis protein AlgF;
DE Flags: Precursor;
GN Name=algF;
OS Pseudomonas fluorescens.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=294;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 17397 / DSM 50091 / CIP 73.25 / NCIMB 10525 / 12;
RX PubMed=12775688; DOI=10.1128/jb.185.12.3515-3523.2003;
RA Gimmestad M., Sletta H., Ertesvaag H., Bakkevig K., Jain S., Suh S.-J.,
RA Skjaak-Braek G., Ellingsen T.E., Ohman D.E., Valla S.;
RT "The Pseudomonas fluorescens AlgG protein, but not its mannuronan C-5-
RT epimerase activity, is needed for alginate polymer formation.";
RL J. Bacteriol. 185:3515-3523(2003).
CC -!- FUNCTION: Together with AlgI and AlgJ, forms an inner membrane complex
CC which probably interacts with the alginate polymerization-transport
CC complex and adds acetyl groups at the O-2 and O-3 positions of
CC mannuronate residues. Acetylation of alginate is important for the
CC architecture of biofilms and increases the ability of alginate to act
CC as a defense barrier (By similarity). {ECO:0000250}.
CC -!- PATHWAY: Glycan biosynthesis; alginate biosynthesis.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the AlgF family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF527790; AAP46699.1; -; Genomic_DNA.
DR RefSeq; WP_012722326.1; NZ_CABVHZ010000006.1.
DR AlphaFoldDB; P59791; -.
DR SMR; P59791; -.
DR STRING; 690597.JH730920_gene1197; -.
DR PATRIC; fig|294.129.peg.5010; -.
DR eggNOG; ENOG5032T05; Bacteria.
DR UniPathway; UPA00286; -.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0042121; P:alginic acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR035422; AlgF.
DR Pfam; PF11182; AlgF; 1.
PE 3: Inferred from homology;
KW Alginate biosynthesis; Periplasm; Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..217
FT /note="Alginate biosynthesis protein AlgF"
FT /id="PRO_0000001116"
SQ SEQUENCE 217 AA; 22808 MW; 7277C8D804C547F3 CRC64;
MTFTTTPRRL AKTLALAAGM SVVSLSAFAG DAALYGPVAP KGSSFVRVYN ASNQEVSATV
GSTNLSEVAP LASSDFSFMP GGDYSAKIGS QTVPVKLAAD HYYTLVNNSS GQPQLIEEPP
FKNKQKSLVR VQNLSDKALT LKTADGKTDV VKSVAAKGRG EREINPVKVS LALYDGDKKV
GDVKPVALER GEAAVLYVTG SGSSISPVWV KRPVSTR