ID   ALGF_PSEPK              Reviewed;         215 AA.
AC   Q88ND4;
DT   25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Alginate biosynthesis protein AlgF;
DE   Flags: Precursor;
GN   Name=algF; OrderedLocusNames=PP_1278;
OS   Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS   / KT2440).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=160488;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX   PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA   Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA   Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA   Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA   Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M., Hance I.,
RA   Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A.,
RA   Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H.,
RA   Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C.,
RA   Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.;
RT   "Complete genome sequence and comparative analysis of the metabolically
RT   versatile Pseudomonas putida KT2440.";
RL   Environ. Microbiol. 4:799-808(2002).
CC   -!- FUNCTION: Together with AlgI and AlgJ, forms an inner membrane complex
CC       which probably interacts with the alginate polymerization-transport
CC       complex and adds acetyl groups at the O-2 and O-3 positions of
CC       mannuronate residues. Acetylation of alginate is important for the
CC       architecture of biofilms and increases the ability of alginate to act
CC       as a defense barrier (By similarity). {ECO:0000250}.
CC   -!- PATHWAY: Glycan biosynthesis; alginate biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the AlgF family. {ECO:0000305}.
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DR   EMBL; AE015451; AAN66902.1; -; Genomic_DNA.
DR   RefSeq; NP_743438.1; NC_002947.4.
DR   RefSeq; WP_003251744.1; NC_002947.4.
DR   AlphaFoldDB; Q88ND4; -.
DR   SMR; Q88ND4; -.
DR   STRING; 160488.PP_1278; -.
DR   EnsemblBacteria; AAN66902; AAN66902; PP_1278.
DR   KEGG; ppu:PP_1278; -.
DR   PATRIC; fig|160488.4.peg.1355; -.
DR   eggNOG; ENOG5032T05; Bacteria.
DR   HOGENOM; CLU_112428_0_0_6; -.
DR   OMA; SSDFSFM; -.
DR   PhylomeDB; Q88ND4; -.
DR   BioCyc; PPUT160488:G1G01-1365-MON; -.
DR   UniPathway; UPA00286; -.
DR   Proteomes; UP000000556; Chromosome.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0042121; P:alginic acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR035422; AlgF.
DR   Pfam; PF11182; AlgF; 1.
PE   3: Inferred from homology;
KW   Alginate biosynthesis; Periplasm; Reference proteome; Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..215
FT                   /note="Alginate biosynthesis protein AlgF"
FT                   /id="PRO_0000001117"
SQ   SEQUENCE   215 AA;  22282 MW;  0A2A3B6AF428BB26 CRC64;
     MTTKTSIAKA LTLAAGLSLA SMQAFAGADA ALYGPSAPKG STFVRLYNAT SAPAAASVGN
     TQIKQVGAQA SSDFSFLPGG DYTAQVGGKS VPVKLASDKY YTLVNSNSGS PKLIEEPPFK
     NKQKALVRVQ NLSDQQLTLK TADGKTEVVK PVAANGRGER EINPVKVNLA LYQGDKKVGD
     VKPVALERGE AAVLYVTGSG NALSPVWVTR PVASN