GALR2_MOUSE
ID GALR2_MOUSE Reviewed; 371 AA.
AC O88854; Q14A53; Q9Z2B0;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Galanin receptor type 2;
DE Short=GAL2-R;
DE Short=GALR-2;
GN Name=Galr2; Synonyms=Galnr2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=129/Sv;
RX PubMed=9832121; DOI=10.1046/j.1471-4159.1998.71062239.x;
RA Kolakowski L.F. Jr., O'Neill G.P., Howard A.D., Broussard S.R.,
RA Sullivan K.A., Feighner S.D., Sawzdargo M., Nguyen T., Kargman S.,
RA Shiao L.-L., Hreniuk D.L., Tan C.P., Evans J., Abramovitz M.,
RA Chateauneuf A., Coulombe N., Ng G., Johnson M.P., Tharian A.,
RA Khoshbouei H., George S.R., Smith R.G., O'Dowd B.F.;
RT "Molecular characterization and expression of cloned human galanin
RT receptors GALR2 and GALR3.";
RL J. Neurochem. 71:2239-2251(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=129/Sv;
RX PubMed=9832122; DOI=10.1046/j.1471-4159.1998.71062252.x;
RA Pang L., Hashemi T., Lee H.J., Maguire M., Graziano M.P., Bayne M.,
RA Hawes B., Wong G., Wang S.;
RT "The mouse GalR2 galanin receptor: genomic organization, cDNA cloning, and
RT functional characterization.";
RL J. Neurochem. 71:2252-2259(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Receptor for the hormone galanin, GALP and spexin-1. The
CC activity of this receptor is mediated by G proteins that activate the
CC phospholipase C/protein kinase C pathway (via G(q)) and that inhibit
CC adenylyl cyclase (via G(i)). {ECO:0000250|UniProtKB:O43603,
CC ECO:0000269|PubMed:9832122}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; AF042784; AAC36589.1; -; Genomic_DNA.
DR EMBL; AF077375; AAC95468.1; -; mRNA.
DR EMBL; AL645861; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466558; EDL34569.1; -; Genomic_DNA.
DR EMBL; BC116980; AAI16981.1; -; mRNA.
DR EMBL; BC116982; AAI16983.1; -; mRNA.
DR CCDS; CCDS25663.1; -.
DR RefSeq; NP_034384.3; NM_010254.4.
DR AlphaFoldDB; O88854; -.
DR SMR; O88854; -.
DR STRING; 10090.ENSMUSP00000054062; -.
DR GuidetoPHARMACOLOGY; 244; -.
DR GlyGen; O88854; 1 site.
DR PhosphoSitePlus; O88854; -.
DR PaxDb; O88854; -.
DR PRIDE; O88854; -.
DR Antibodypedia; 19663; 297 antibodies from 37 providers.
DR DNASU; 14428; -.
DR Ensembl; ENSMUST00000055872; ENSMUSP00000054062; ENSMUSG00000020793.
DR GeneID; 14428; -.
DR KEGG; mmu:14428; -.
DR UCSC; uc007mkr.2; mouse.
DR CTD; 8811; -.
DR MGI; MGI:1337018; Galr2.
DR VEuPathDB; HostDB:ENSMUSG00000020793; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01050000244983; -.
DR HOGENOM; CLU_009579_6_4_1; -.
DR InParanoid; O88854; -.
DR OMA; DICTFVF; -.
DR OrthoDB; 1294084at2759; -.
DR PhylomeDB; O88854; -.
DR TreeFam; TF315737; -.
DR Reactome; R-MMU-375276; Peptide ligand-binding receptors.
DR Reactome; R-MMU-418594; G alpha (i) signalling events.
DR BioGRID-ORCS; 14428; 6 hits in 75 CRISPR screens.
DR PRO; PR:O88854; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; O88854; protein.
DR Bgee; ENSMUSG00000020793; Expressed in embryonic brain and 43 other tissues.
DR Genevisible; O88854; MM.
DR GO; GO:0005929; C:cilium; ISO:MGI.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0008528; F:G protein-coupled peptide receptor activity; IBA:GO_Central.
DR GO; GO:0004966; F:galanin receptor activity; ISS:UniProtKB.
DR GO; GO:0042923; F:neuropeptide binding; IEA:Ensembl.
DR GO; GO:0017046; F:peptide hormone binding; ISS:UniProtKB.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IEA:Ensembl.
DR GO; GO:0007188; P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0090663; P:galanin-activated signaling pathway; ISO:MGI.
DR GO; GO:0043647; P:inositol phosphate metabolic process; IEA:Ensembl.
DR GO; GO:0007194; P:negative regulation of adenylate cyclase activity; IEA:InterPro.
DR GO; GO:0031175; P:neuron projection development; IDA:MGI.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IBA:GO_Central.
DR GO; GO:0046488; P:phosphatidylinositol metabolic process; IEA:Ensembl.
DR GO; GO:0007200; P:phospholipase C-activating G protein-coupled receptor signaling pathway; ISO:MGI.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IEA:Ensembl.
DR GO; GO:1902608; P:positive regulation of large conductance calcium-activated potassium channel activity; ISO:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR InterPro; IPR003907; GAL2_rcpt.
DR InterPro; IPR000405; Galanin_rcpt.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR01419; GALANIN2R.
DR PRINTS; PR00663; GALANINR.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..371
FT /note="Galanin receptor type 2"
FT /id="PRO_0000069467"
FT TOPO_DOM 1..27
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 28..48
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 49..59
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 81..98
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..120
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 121..140
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..161
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 162..186
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 208..236
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 237..257
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 258..259
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 260..280
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 281..371
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 2
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 97..174
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT CONFLICT 130
FT /note="L -> M (in Ref. 1; AAC36589)"
FT /evidence="ECO:0000305"
FT CONFLICT 184..188
FT /note="RAMDL -> PWNS (in Ref. 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 192..193
FT /note="VF -> CL (in Ref. 2; AAC95468)"
FT /evidence="ECO:0000305"
FT CONFLICT 221
FT /note="A -> V (in Ref. 2; AAC95468)"
FT /evidence="ECO:0000305"
FT CONFLICT 345..360
FT /note="SEAAGPLVPAPALPNC -> KRGSRAPRPRTRTSQT (in Ref. 2)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 371 AA; 40516 MW; C59F6560AF2EFD34 CRC64;
MNGSDSQGAE DSSQEGGGGW QPEAVLVPLF FALIFLVGAV GNALVLAVLL RGGQAVSTTN
LFILNLGVAD LCFILCCVPF QATIYTLDDW VFGSLLCKAV HFLIFLTMHA SSFTLAAVSL
DRYLAIRYPL HSRELRTPRN ALAAIGLIWG LALLFSGPYL SYYSQSQLAN LTVCHPAWSA
PRRRAMDLCT FVFSYLLPVL VLSLTYARTL HYLWRTVDPV AAGSGSQRAK RKVTRMIVIV
AVLFCLCWMP HHALILCVWF GRFPLTRATY ALRILSHLVS YANSCVNPIV YALVSKHFRK
GFRKICAGLL RRAPRRASGR VCILAPGNHS GGMLEPESTD LTQVSEAAGP LVPAPALPNC
TTLSRTLDPA C
