GALR3_HUMAN
ID GALR3_HUMAN Reviewed; 368 AA.
AC O60755; Q53YJ4;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 171.
DE RecName: Full=Galanin receptor type 3;
DE Short=GAL3-R;
DE Short=GALR-3;
GN Name=GALR3; Synonyms=GALNR3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX PubMed=9722565; DOI=10.1074/jbc.273.36.23321;
RA Smith K.E., Walker M.W., Artymyshyn R., Bard J., Borowsky B., Tamm J.A.,
RA Yao W.-J., Vaysse P.J.-J., Branchek T.A., Gerald C., Jones K.A.;
RT "Cloned human and rat galanin GALR3 receptors: pharmacology and activation
RT of G-protein inwardly rectifying K+ channels.";
RL J. Biol. Chem. 273:23321-23326(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Uterus;
RA Bennett M.M., Lescoe M.K., Gallipoli P.Z., Ramabhadran T.V.;
RT "Homologue of the human galanin 2 receptor gene isolated from a human
RT uterus cDNA library.";
RL Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=9832121; DOI=10.1046/j.1471-4159.1998.71062239.x;
RA Kolakowski L.F. Jr., O'Neill G.P., Howard A.D., Broussard S.R.,
RA Sullivan K.A., Feighner S.D., Sawzdargo M., Nguyen T., Kargman S.,
RA Shiao L.-L., Hreniuk D.L., Tan C.P., Evans J., Abramovitz M.,
RA Chateauneuf A., Coulombe N., Ng G., Johnson M.P., Tharian A.,
RA Khoshbouei H., George S.R., Smith R.G., O'Dowd B.F.;
RT "Molecular characterization and expression of cloned human galanin
RT receptors GALR2 and GALR3.";
RL J. Neurochem. 71:2239-2251(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9928159; DOI=10.1111/j.1749-6632.1998.tb10683.x;
RA Iismaa T.P., Fathi Z., Hort Y.J., Iben L.G., Dutton J.L., Baker E.,
RA Sutherland G.R., Shine J.;
RT "Structural organization and chromosomal localization of three human
RT galanin receptor genes.";
RL Ann. N. Y. Acad. Sci. 863:56-63(1998).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Retina;
RA Eutsler E.P., Kopatz S.A., Sharma S.V.;
RT "Isolation of cDNA coding for galanin receptor 3 (GALR3).";
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S.,
RA Tsutsumi S., Aburatani H., Asai K., Akiyama Y.;
RT "Genome-wide discovery and analysis of human seven transmembrane helix
RT receptor genes.";
RL Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=10591208; DOI=10.1038/990031;
RA Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M.,
RA Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C.,
RA Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E.,
RA Bridgeman A.M., Buck D., Burgess J., Burrill W.D., Burton J., Carder C.,
RA Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G.,
RA Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V.,
RA Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M.,
RA Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A.,
RA Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C.,
RA Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E.,
RA Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F.,
RA Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M.,
RA Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A.,
RA Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D.,
RA Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y.,
RA Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S.,
RA Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E.,
RA Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L.,
RA Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L.,
RA Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N.,
RA Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A.,
RA Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L.,
RA Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P.,
RA Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P.,
RA Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q.,
RA Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J.,
RA Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J.,
RA Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D.,
RA Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T.,
RA Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P.,
RA Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K.,
RA Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R.,
RA Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L.,
RA McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J.,
RA Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E.,
RA Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P.,
RA Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y.,
RA Wright H.;
RT "The DNA sequence of human chromosome 22.";
RL Nature 402:489-495(1999).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [9]
RP FUNCTION AS A RECEPTOR FOR SPEXIN-1.
RX PubMed=24517231; DOI=10.1210/en.2013-2106;
RA Kim D.K., Yun S., Son G.H., Hwang J.I., Park C.R., Kim J.I., Kim K.,
RA Vaudry H., Seong J.Y.;
RT "Coevolution of the spexin/galanin/kisspeptin family: Spexin activates
RT galanin receptor type II and III.";
RL Endocrinology 155:1864-1873(2014).
RN [10]
RP FUNCTION.
RX PubMed=25691535; DOI=10.1093/hmg/ddv060;
RA Guipponi M., Chentouf A., Webling K.E., Freimann K., Crespel A., Nobile C.,
RA Lemke J.R., Hansen J., Dorn T., Lesca G., Ryvlin P., Hirsch E., Rudolf G.,
RA Rosenberg D.S., Weber Y., Becker F., Helbig I., Muhle H., Salzmann A.,
RA Chaouch M., Oubaiche M.L., Ziglio S., Gehrig C., Santoni F., Pizzato M.,
RA Langel U., Antonarakis S.E.;
RT "Galanin pathogenic mutations in temporal lobe epilepsy.";
RL Hum. Mol. Genet. 24:3082-3091(2015).
CC -!- FUNCTION: Receptor for the hormone galanin (PubMed:25691535). Receptor
CC for the hormone spexin-1 (PubMed:24517231).
CC {ECO:0000269|PubMed:24517231, ECO:0000269|PubMed:25691535,
CC ECO:0000269|PubMed:9722565, ECO:0000269|PubMed:9832121}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; AF073799; AAC35944.1; -; mRNA.
DR EMBL; AF067733; AAC18860.1; -; mRNA.
DR EMBL; AF129514; AAD47348.2; -; Genomic_DNA.
DR EMBL; AF129513; AAD47348.2; JOINED; Genomic_DNA.
DR EMBL; AY587582; AAS98215.1; -; mRNA.
DR EMBL; AB065934; BAC06149.1; -; Genomic_DNA.
DR EMBL; Z97630; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471095; EAW60191.1; -; Genomic_DNA.
DR CCDS; CCDS13958.1; -.
DR RefSeq; NP_003605.1; NM_003614.1.
DR AlphaFoldDB; O60755; -.
DR SMR; O60755; -.
DR BioGRID; 114058; 30.
DR STRING; 9606.ENSP00000249041; -.
DR BindingDB; O60755; -.
DR ChEMBL; CHEMBL2731; -.
DR GuidetoPHARMACOLOGY; 245; -.
DR GlyGen; O60755; 1 site.
DR iPTMnet; O60755; -.
DR PhosphoSitePlus; O60755; -.
DR BioMuta; GALR3; -.
DR MassIVE; O60755; -.
DR PaxDb; O60755; -.
DR PeptideAtlas; O60755; -.
DR PRIDE; O60755; -.
DR Antibodypedia; 12147; 245 antibodies from 32 providers.
DR DNASU; 8484; -.
DR Ensembl; ENST00000249041.3; ENSP00000249041.2; ENSG00000128310.3.
DR GeneID; 8484; -.
DR KEGG; hsa:8484; -.
DR MANE-Select; ENST00000249041.3; ENSP00000249041.2; NM_003614.2; NP_003605.1.
DR UCSC; uc003aub.1; human.
DR CTD; 8484; -.
DR DisGeNET; 8484; -.
DR GeneCards; GALR3; -.
DR HGNC; HGNC:4134; GALR3.
DR HPA; ENSG00000128310; Not detected.
DR MIM; 603692; gene.
DR neXtProt; NX_O60755; -.
DR OpenTargets; ENSG00000128310; -.
DR PharmGKB; PA28547; -.
DR VEuPathDB; HostDB:ENSG00000128310; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01050000244841; -.
DR HOGENOM; CLU_009579_6_4_1; -.
DR InParanoid; O60755; -.
DR OMA; GCPGDAR; -.
DR OrthoDB; 1294084at2759; -.
DR PhylomeDB; O60755; -.
DR TreeFam; TF315737; -.
DR PathwayCommons; O60755; -.
DR Reactome; R-HSA-375276; Peptide ligand-binding receptors.
DR Reactome; R-HSA-418594; G alpha (i) signalling events.
DR SignaLink; O60755; -.
DR SIGNOR; O60755; -.
DR BioGRID-ORCS; 8484; 16 hits in 1064 CRISPR screens.
DR ChiTaRS; GALR3; human.
DR GeneWiki; Galanin_receptor_3; -.
DR GenomeRNAi; 8484; -.
DR Pharos; O60755; Tchem.
DR PRO; PR:O60755; -.
DR Proteomes; UP000005640; Chromosome 22.
DR RNAct; O60755; protein.
DR Bgee; ENSG00000128310; Expressed in cerebellar hemisphere and 20 other tissues.
DR Genevisible; O60755; HS.
DR GO; GO:0005929; C:cilium; IDA:MGI.
DR GO; GO:0016021; C:integral component of membrane; TAS:ProtInc.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0097730; C:non-motile cilium; IEA:Ensembl.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0008528; F:G protein-coupled peptide receptor activity; IBA:GO_Central.
DR GO; GO:0004966; F:galanin receptor activity; IDA:UniProtKB.
DR GO; GO:0017046; F:peptide hormone binding; IDA:UniProtKB.
DR GO; GO:0007188; P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007268; P:chemical synaptic transmission; TAS:ProtInc.
DR GO; GO:0007631; P:feeding behavior; TAS:ProtInc.
DR GO; GO:0007187; P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger; IDA:GO_Central.
DR GO; GO:0090663; P:galanin-activated signaling pathway; IDA:GO_Central.
DR GO; GO:0007611; P:learning or memory; TAS:ProtInc.
DR GO; GO:0007194; P:negative regulation of adenylate cyclase activity; IEA:InterPro.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR InterPro; IPR000405; Galanin_rcpt.
DR InterPro; IPR003908; Galnin_3_rcpt.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR01420; GALANIN3R.
DR PRINTS; PR00663; GALANINR.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Lipoprotein; Membrane; Palmitate; Receptor; Reference proteome; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..368
FT /note="Galanin receptor type 3"
FT /id="PRO_0000069469"
FT TOPO_DOM 1..20
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 21..41
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 42..57
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 58..78
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 79..96
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 97..118
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 119..138
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 160..184
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 206..236
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 237..257
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 258..259
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 260..280
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 281..368
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 317..368
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 308
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000250"
FT CARBOHYD 6
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 95..172
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT VARIANT 342
FT /note="R -> G (in dbSNP:rs8137541)"
FT /id="VAR_049387"
FT VARIANT 349
FT /note="Q -> R (in dbSNP:rs8137553)"
FT /id="VAR_059322"
SQ SEQUENCE 368 AA; 39573 MW; E8F46B66422C3150 CRC64;
MADAQNISLD SPGSVGAVAV PVVFALIFLL GTVGNGLVLA VLLQPGPSAW QEPGSTTDLF
ILNLAVADLC FILCCVPFQA TIYTLDAWLF GALVCKAVHL LIYLTMYASS FTLAAVSVDR
YLAVRHPLRS RALRTPRNAR AAVGLVWLLA ALFSAPYLSY YGTVRYGALE LCVPAWEDAR
RRALDVATFA AGYLLPVAVV SLAYGRTLRF LWAAVGPAGA AAAEARRRAT GRAGRAMLAV
AALYALCWGP HHALILCFWY GRFAFSPATY ACRLASHCLA YANSCLNPLV YALASRHFRA
RFRRLWPCGR RRRHRARRAL RRVRPASSGP PGCPGDARPS GRLLAGGGQG PEPREGPVHG
GEAARGPE
