GALR_ECOLI
ID GALR_ECOLI Reviewed; 343 AA.
AC P03024; Q2MA00;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 25-MAY-2022, entry version 171.
DE RecName: Full=HTH-type transcriptional regulator GalR;
DE AltName: Full=Galactose operon repressor;
GN Name=galR; OrderedLocusNames=b2837, JW2805;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6283521; DOI=10.1073/pnas.79.8.2427;
RA von Wilcken-Bergmann B., Mueller-Hill B.;
RT "Sequence of galR gene indicates a common evolutionary origin of lac and
RT gal repressor in Escherichia coli.";
RL Proc. Natl. Acad. Sci. U.S.A. 79:2427-2431(1982).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-343.
RX PubMed=6350601; DOI=10.1016/s0022-2836(83)80021-7;
RA Stragier P., Danos O., Patte J.-C.;
RT "Regulation of diaminopimelate decarboxylase synthesis in Escherichia coli.
RT II. Nucleotide sequence of the lysA gene and its regulatory region.";
RL J. Mol. Biol. 168:321-331(1983).
RN [5]
RP 3D-STRUCTURE MODELING OF 58-343.
RX PubMed=8188660; DOI=10.1016/s0021-9258(17)36722-4;
RA Hsieh M., Hensely P., Brenowitz M., Fetrow J.S.;
RT "A molecular model of the inducer binding domain of the galactose repressor
RT of Escherichia coli.";
RL J. Biol. Chem. 269:13825-13835(1994).
RN [6]
RP CHARACTERIZATION.
RX PubMed=8982002; DOI=10.1128/jb.179.1.228-234.1997;
RA Geanacopoulos M., Adhya S.;
RT "Functional characterization of roles of GalR and GalS as regulators of the
RT gal regulon.";
RL J. Bacteriol. 179:228-234(1997).
CC -!- FUNCTION: Repressor of the galactose operon. Binds galactose as an
CC inducer.
CC -!- PATHWAY: Carbohydrate metabolism; galactose metabolism [regulation].
CC -!- SUBUNIT: Homodimer.
CC -!- INTERACTION:
CC P03024; P03024: galR; NbExp=3; IntAct=EBI-556902, EBI-556902;
CC P03024; P25748: galS; NbExp=5; IntAct=EBI-556902, EBI-1117006;
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DR EMBL; V00280; CAA23543.1; -; Genomic_DNA.
DR EMBL; U29581; AAB40484.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75876.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76906.1; -; Genomic_DNA.
DR EMBL; J01614; AAA83860.1; -; Genomic_DNA.
DR PIR; A93910; RPECG.
DR RefSeq; NP_417314.1; NC_000913.3.
DR RefSeq; WP_000201063.1; NZ_SSZK01000003.1.
DR AlphaFoldDB; P03024; -.
DR SMR; P03024; -.
DR BioGRID; 4262964; 20.
DR BioGRID; 851641; 2.
DR DIP; DIP-9733N; -.
DR IntAct; P03024; 3.
DR STRING; 511145.b2837; -.
DR jPOST; P03024; -.
DR PaxDb; P03024; -.
DR PRIDE; P03024; -.
DR EnsemblBacteria; AAC75876; AAC75876; b2837.
DR EnsemblBacteria; BAE76906; BAE76906; BAE76906.
DR GeneID; 947314; -.
DR KEGG; ecj:JW2805; -.
DR KEGG; eco:b2837; -.
DR PATRIC; fig|1411691.4.peg.3897; -.
DR EchoBASE; EB0359; -.
DR eggNOG; COG1609; Bacteria.
DR HOGENOM; CLU_037628_6_0_6; -.
DR InParanoid; P03024; -.
DR OMA; CVAVDFR; -.
DR PhylomeDB; P03024; -.
DR BioCyc; EcoCyc:PD03028; -.
DR UniPathway; UPA00214; -.
DR PRO; PR:P03024; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IDA:EcoCyc.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0006012; P:galactose metabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:EcoCyc.
DR GO; GO:0006351; P:transcription, DNA-templated; IDA:EcoCyc.
DR CDD; cd01392; HTH_LacI; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR000843; HTH_LacI.
DR InterPro; IPR046335; LacI/GalR-like_sensor.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR028082; Peripla_BP_I.
DR Pfam; PF00356; LacI; 1.
DR Pfam; PF13377; Peripla_BP_3; 1.
DR PRINTS; PR00036; HTHLACI.
DR SMART; SM00354; HTH_LACI; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR SUPFAM; SSF53822; SSF53822; 1.
DR PROSITE; PS00356; HTH_LACI_1; 1.
DR PROSITE; PS50932; HTH_LACI_2; 1.
PE 1: Evidence at protein level;
KW Carbohydrate metabolism; DNA-binding; Galactose metabolism;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..343
FT /note="HTH-type transcriptional regulator GalR"
FT /id="PRO_0000107951"
FT DOMAIN 1..56
FT /note="HTH lacI-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00111"
FT DNA_BIND 4..23
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00111"
SQ SEQUENCE 343 AA; 37094 MW; A4855FC8A2527A30 CRC64;
MATIKDVARL AGVSVATVSR VINNSPKASE ASRLAVHSAM ESLSYHPNAN ARALAQQTTE
TVGLVVGDVS DPFFGAMVKA VEQVAYHTGN FLLIGNGYHN EQKERQAIEQ LIRHRCAALV
VHAKMIPDAD LASLMKQMPG MVLINRILPG FENRCIALDD RYGAWLATRH LIQQGHTRIG
YLCSNHSISD AEDRLQGYYD ALAESGIAAN DRLVTFGEPD ESGGEQAMTE LLGRGRNFTA
VACYNDSMAA GAMGVLNDNG IDVPGEISLI GFDDVLVSRY VRPRLTTVRY PIVTMATQAA
ELALALADNR PLPEITNVFS PTLVRRHSVS TPSLEASHHA TSD
