ID   GALSF_BACTN             Reviewed;         511 AA.
AC   Q8A2H2;
DT   13-FEB-2019, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=N-acetylgalactosamine-6-O-sulfatase {ECO:0000303|PubMed:25002587};
DE            EC=3.1.6.- {ECO:0000269|PubMed:25002587};
GN   OrderedLocusNames=BT_3333 {ECO:0000312|EMBL:AAO78439.1};
OS   Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 /
OS   CCUG 10774 / NCTC 10582 / VPI-5482 / E50).
OC   Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC   Bacteroides.
OX   NCBI_TaxID=226186;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 /
RC   VPI-5482 / E50;
RX   PubMed=12663928; DOI=10.1126/science.1080029;
RA   Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C.,
RA   Hooper L.V., Gordon J.I.;
RT   "A genomic view of the human-Bacteroides thetaiotaomicron symbiosis.";
RL   Science 299:2074-2076(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 /
RC   VPI-5482 / E50;
RX   PubMed=19321416; DOI=10.1073/pnas.0901529106;
RA   Mahowald M.A., Rey F.E., Seedorf H., Turnbaugh P.J., Fulton R.S.,
RA   Wollam A., Shah N., Wang C., Magrini V., Wilson R.K., Cantarel B.L.,
RA   Coutinho P.M., Henrissat B., Crock L.W., Russell A., Verberkmoes N.C.,
RA   Hettich R.L., Gordon J.I.;
RT   "Characterizing a model human gut microbiota composed of members of its two
RT   dominant bacterial phyla.";
RL   Proc. Natl. Acad. Sci. U.S.A. 106:5859-5864(2009).
RN   [3]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=25002587; DOI=10.1074/jbc.m114.573303;
RA   Ulmer J.E., Vilen E.M., Namburi R.B., Benjdia A., Beneteau J., Malleron A.,
RA   Bonnaffe D., Driguez P.A., Descroix K., Lassalle G., Le Narvor C.,
RA   Sandstroem C., Spillmann D., Berteau O.;
RT   "Characterization of glycosaminoglycan (GAG) sulfatases from the human gut
RT   symbiont Bacteroides thetaiotaomicron reveals the first GAG-specific
RT   bacterial endosulfatase.";
RL   J. Biol. Chem. 289:24289-24303(2014).
CC   -!- FUNCTION: Exosulfatase involved in the degradation of the
CC       glycosaminoglycans (GAGs) chondroitin sulfate (CS) and dermatan sulfate
CC       (DS). Catalyzes the hydrolysis of the 6-sulfate groups of the N-acetyl-
CC       D-galactosamine 6-sulfate units (PubMed:25002587). GAG-specific
CC       sulfatases play a key role in the persistence of the major human gut
CC       symbiont B.thetaiotaomicron in the host gastrointestinal tract
CC       (PubMed:25002587). {ECO:0000269|PubMed:25002587,
CC       ECO:0000305|PubMed:25002587}.
CC   -!- PTM: The conversion to 3-oxoalanine (also known as C-formylglycine,
CC       FGly), of a serine or cysteine residue in prokaryotes and of a cysteine
CC       residue in eukaryotes, is critical for catalytic activity.
CC       {ECO:0000250|UniProtKB:Q9X759}.
CC   -!- SIMILARITY: Belongs to the sulfatase family. {ECO:0000305}.
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DR   EMBL; AE015928; AAO78439.1; -; Genomic_DNA.
DR   RefSeq; NP_812245.1; NC_004663.1.
DR   RefSeq; WP_011108775.1; NC_004663.1.
DR   PDB; 6S20; X-ray; 1.98 A; C=22-511.
DR   PDBsum; 6S20; -.
DR   AlphaFoldDB; Q8A2H2; -.
DR   SMR; Q8A2H2; -.
DR   STRING; 226186.BT_3333; -.
DR   PaxDb; Q8A2H2; -.
DR   PRIDE; Q8A2H2; -.
DR   EnsemblBacteria; AAO78439; AAO78439; BT_3333.
DR   GeneID; 60924512; -.
DR   KEGG; bth:BT_3333; -.
DR   PATRIC; fig|226186.12.peg.3400; -.
DR   eggNOG; COG3119; Bacteria.
DR   HOGENOM; CLU_006332_10_3_10; -.
DR   InParanoid; Q8A2H2; -.
DR   OMA; IPRIGWM; -.
DR   Proteomes; UP000001414; Chromosome.
DR   GO; GO:0008484; F:sulfuric ester hydrolase activity; IEA:InterPro.
DR   Gene3D; 3.40.720.10; -; 1.
DR   InterPro; IPR017850; Alkaline_phosphatase_core_sf.
DR   InterPro; IPR032158; DUF4994.
DR   InterPro; IPR024607; Sulfatase_CS.
DR   InterPro; IPR000917; Sulfatase_N.
DR   Pfam; PF16385; DUF4994; 1.
DR   Pfam; PF00884; Sulfatase; 1.
DR   SUPFAM; SSF53649; SSF53649; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
DR   PROSITE; PS00523; SULFATASE_1; 1.
DR   PROSITE; PS00149; SULFATASE_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Hydrolase; Reference proteome.
FT   CHAIN           1..511
FT                   /note="N-acetylgalactosamine-6-O-sulfatase"
FT                   /id="PRO_0000446230"
FT   MOD_RES         83
FT                   /note="3-oxoalanine (Ser)"
FT                   /evidence="ECO:0000250|UniProtKB:Q9X759"
FT   STRAND          36..44
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   HELIX           51..53
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   STRAND          56..58
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   HELIX           61..69
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   STRAND          70..77
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   STRAND          79..82
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   HELIX           83..92
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   HELIX           96..98
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   HELIX           120..126
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   STRAND          130..136
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   HELIX           157..160
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   STRAND          163..170
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   STRAND          195..200
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   TURN            208..210
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   HELIX           212..214
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   STRAND          219..222
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   STRAND          225..228
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   STRAND          234..239
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   HELIX           241..243
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   HELIX           247..249
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   HELIX           250..264
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   TURN            265..267
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   STRAND          270..275
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   STRAND          280..282
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   TURN            287..291
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   STRAND          292..295
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   HELIX           296..318
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   STRAND          325..333
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   STRAND          339..341
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   TURN            345..348
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   TURN            354..357
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   HELIX           367..369
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   STRAND          374..377
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   TURN            379..381
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   STRAND          385..387
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   HELIX           393..395
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   HELIX           396..403
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   HELIX           420..423
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   STRAND          434..437
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   STRAND          443..447
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   STRAND          450..453
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   TURN            463..465
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   STRAND          476..479
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   TURN            480..482
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   TURN            491..493
FT                   /evidence="ECO:0007829|PDB:6S20"
FT   HELIX           495..510
FT                   /evidence="ECO:0007829|PDB:6S20"
SQ   SEQUENCE   511 AA;  56324 MW;  3919271135DD47B1 CRC64;
     MKNVSRLLPL LPGIALLTGC NQKVQKDNGQ NSQKPNIIYI FADDLGIGDL SCYGATKVST
     PHIDRLAGQG VQFTNAYATS ATSTPSRFGL LTGMYPWRQE NTGIAPGNSE LIIDTACVTM
     ADMLKEAGYA TGVVGKWHLG LGPKGGTDFN GHITPNAQSI GFDYEFVIPA TVDRVPCVFV
     ENGHVVGLDP NDPITVNYEH KVGDWPTGEE NPELVKLKPS QGHNNTIING IPRIGWMTGG
     KSALWKDEDI ADIITNKAKS FIVSHKEEPF FLYMGTQDVH VPRVPHPRFA GKSGLGTRGD
     VILQLDWTIG EIMNTLDSLQ LTDNTILIFT SDNGPVIDDG YQDQAFERLN GHTPMGIYRG
     GKYSAYEAGT RIPFIVRWPA KVKPNKQQAL FSQIDIFASL AALLKQPLPE DAAPDSQEHL
     NTLLGKDYTS REYIVQQNLN NTLAIVKGQW KYIEPSDAPA IEYWTKMELG NDRHPQLYDL
     SADPSEKNNV AKQHPEVVRE LSELLESVKT R