GALS_ECOLI
ID GALS_ECOLI Reviewed; 346 AA.
AC P25748; Q2MAS8;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 25-MAY-2022, entry version 153.
DE RecName: Full=HTH-type transcriptional regulator GalS;
DE AltName: Full=Mgl repressor and galactose ultrainduction factor;
GN Name=galS; OrderedLocusNames=b2151, JW2138;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / C600 / CR34 / ATCC 23724 / DSM 3925 / LMG 3041 / NCIB 10222;
RX PubMed=1619663; DOI=10.1016/0022-2836(92)90125-4;
RA Weickert M.J., Adhya S.;
RT "Isorepressor of the gal regulon in Escherichia coli.";
RL J. Mol. Biol. 226:69-83(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / BHB2600;
RA Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K.,
RA Church G.M.;
RT "Automated multiplex sequencing of the E.coli genome.";
RL Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP DNA-BINDING.
RX PubMed=8208287; DOI=10.1038/369614a0;
RA Mueller-Hill B., Kolkhof P.;
RT "DNA recognition and the code.";
RL Nature 369:614-614(1994).
RN [6]
RP PROTEIN SEQUENCE OF 1-20, AND CHARACTERIZATION.
RX PubMed=8982002; DOI=10.1128/jb.179.1.228-234.1997;
RA Geanacopoulos M., Adhya S.;
RT "Functional characterization of roles of GalR and GalS as regulators of the
RT gal regulon.";
RL J. Bacteriol. 179:228-234(1997).
CC -!- FUNCTION: Repressor of the mgl operon. Binds galactose and D-fucose as
CC inducers. GalS binds to an operator DNA sequence within its own coding
CC sequence (corresponding to residues 15 to 20).
CC -!- SUBUNIT: Homodimer.
CC -!- INTERACTION:
CC P25748; P03024: galR; NbExp=5; IntAct=EBI-1117006, EBI-556902;
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DR EMBL; X62529; CAA44390.1; -; Genomic_DNA.
DR EMBL; U00007; AAA60528.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75212.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76628.1; -; Genomic_DNA.
DR PIR; F64983; F64983.
DR RefSeq; NP_416656.1; NC_000913.3.
DR RefSeq; WP_000628648.1; NZ_LN832404.1.
DR AlphaFoldDB; P25748; -.
DR SMR; P25748; -.
DR BioGRID; 853287; 1.
DR DIP; DIP-9734N; -.
DR IntAct; P25748; 7.
DR STRING; 511145.b2151; -.
DR jPOST; P25748; -.
DR PaxDb; P25748; -.
DR PRIDE; P25748; -.
DR EnsemblBacteria; AAC75212; AAC75212; b2151.
DR EnsemblBacteria; BAE76628; BAE76628; BAE76628.
DR GeneID; 949043; -.
DR KEGG; ecj:JW2138; -.
DR KEGG; eco:b2151; -.
DR PATRIC; fig|1411691.4.peg.90; -.
DR EchoBASE; EB0360; -.
DR eggNOG; COG1609; Bacteria.
DR HOGENOM; CLU_037628_6_0_6; -.
DR InParanoid; P25748; -.
DR OMA; RFFAEPY; -.
DR PhylomeDB; P25748; -.
DR BioCyc; EcoCyc:PD00261; -.
DR PRO; PR:P25748; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IDA:EcoCyc.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:EcoCyc.
DR CDD; cd01392; HTH_LacI; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR000843; HTH_LacI.
DR InterPro; IPR046335; LacI/GalR-like_sensor.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR028082; Peripla_BP_I.
DR Pfam; PF00356; LacI; 1.
DR Pfam; PF13377; Peripla_BP_3; 1.
DR PRINTS; PR00036; HTHLACI.
DR SMART; SM00354; HTH_LACI; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR SUPFAM; SSF53822; SSF53822; 1.
DR PROSITE; PS00356; HTH_LACI_1; 1.
DR PROSITE; PS50932; HTH_LACI_2; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; DNA-binding; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..346
FT /note="HTH-type transcriptional regulator GalS"
FT /id="PRO_0000107956"
FT DOMAIN 1..56
FT /note="HTH lacI-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00111"
FT DNA_BIND 4..23
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00111"
FT CONFLICT 104..105
FT /note="ER -> DG (in Ref. 1; CAA44390)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 346 AA; 37357 MW; F13D9DF41A29ADDA CRC64;
MITIRDVARQ AGVSVATVSR VLNNSTLVSA DTREAVMKAV SELDYRPNAN AQALATQVSD
TIGVVVMDVS DAFFGALVKA VDLVAQQHQK YVLIGNSYHE AEKERHAIEV LIRQRCNALI
VHSKALSDDE LAQFMDNIPG MVLINRVVPG YAHRCVCLDN LSGARMATRM LLNNGHQRIG
YLSSSHGIED DAMRKAGWMS ALKEQDIIPP ESWIGAGTPD MPGGEAAMVE LLGRNLQLTA
VFAYNDNMAA GALTALKDNG IAIPLHLSII GFDDIPIARY TDPQLTTVRY PIASMAKLAT
ELALQGAAGN IDPRASHCFM PTLVRRHSVA TRQNAAAITN STNQAM
