GALT_CAEEL
ID GALT_CAEEL Reviewed; 352 AA.
AC Q27536;
DT 30-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Probable galactose-1-phosphate uridylyltransferase;
DE Short=Gal-1-P uridylyltransferase;
DE EC=2.7.7.12 {ECO:0000250|UniProtKB:P07902};
DE AltName: Full=UDP-glucose--hexose-1-phosphate uridylyltransferase;
GN ORFNames=ZK1058.3;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-D-galactose 1-phosphate + UDP-alpha-D-glucose = alpha-D-
CC glucose 1-phosphate + UDP-alpha-D-galactose; Xref=Rhea:RHEA:13989,
CC ChEBI:CHEBI:58336, ChEBI:CHEBI:58601, ChEBI:CHEBI:58885,
CC ChEBI:CHEBI:66914; EC=2.7.7.12;
CC Evidence={ECO:0000250|UniProtKB:P07902};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000250|UniProtKB:P09148};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000250|UniProtKB:P09148};
CC -!- PATHWAY: Carbohydrate metabolism; galactose metabolism.
CC -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P07902}.
CC -!- SIMILARITY: Belongs to the galactose-1-phosphate uridylyltransferase
CC type 1 family. {ECO:0000305}.
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DR EMBL; Z35604; CAA84679.1; -; Genomic_DNA.
DR PIR; T27677; T27677.
DR RefSeq; NP_497789.1; NM_065388.4.
DR AlphaFoldDB; Q27536; -.
DR SMR; Q27536; -.
DR BioGRID; 40744; 1.
DR STRING; 6239.ZK1058.3; -.
DR EPD; Q27536; -.
DR PaxDb; Q27536; -.
DR PeptideAtlas; Q27536; -.
DR EnsemblMetazoa; ZK1058.3.1; ZK1058.3.1; WBGene00014203.
DR GeneID; 175506; -.
DR KEGG; cel:CELE_ZK1058.3; -.
DR UCSC; ZK1058.3; c. elegans.
DR CTD; 175506; -.
DR WormBase; ZK1058.3; CE01103; WBGene00014203; -.
DR eggNOG; KOG2958; Eukaryota.
DR GeneTree; ENSGT00390000016188; -.
DR HOGENOM; CLU_029960_0_1_1; -.
DR InParanoid; Q27536; -.
DR OMA; HAIYYPP; -.
DR OrthoDB; 1135699at2759; -.
DR PhylomeDB; Q27536; -.
DR Reactome; R-CEL-70370; Galactose catabolism.
DR UniPathway; UPA00214; -.
DR PRO; PR:Q27536; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00014203; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0008108; F:UDP-glucose:hexose-1-phosphate uridylyltransferase activity; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0033499; P:galactose catabolic process via UDP-galactose; IBA:GO_Central.
DR CDD; cd00608; GalT; 1.
DR Gene3D; 3.30.428.10; -; 2.
DR InterPro; IPR001937; GalP_UDPtransf1.
DR InterPro; IPR019779; GalP_UDPtransf1_His-AS.
DR InterPro; IPR005850; GalP_Utransf_C.
DR InterPro; IPR005849; GalP_Utransf_N.
DR InterPro; IPR036265; HIT-like_sf.
DR PANTHER; PTHR11943; PTHR11943; 1.
DR Pfam; PF02744; GalP_UDP_tr_C; 1.
DR Pfam; PF01087; GalP_UDP_transf; 1.
DR PIRSF; PIRSF000808; GalT; 1.
DR SUPFAM; SSF54197; SSF54197; 2.
DR TIGRFAMs; TIGR00209; galT_1; 1.
DR PROSITE; PS00117; GAL_P_UDP_TRANSF_I; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Galactose metabolism; Metal-binding;
KW Nucleotidyltransferase; Reference proteome; Transferase; Zinc.
FT CHAIN 1..352
FT /note="Probable galactose-1-phosphate uridylyltransferase"
FT /id="PRO_0000169885"
FT REGION 33..57
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 167
FT /note="Tele-UMP-histidine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10033"
FT BINDING 77..78
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /ligand_note="ligand shared between dimeric partners"
FT /note="in other chain"
FT /evidence="ECO:0000250|UniProtKB:P07902"
FT BINDING 154
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /ligand_note="ligand shared between dimeric partners"
FT /evidence="ECO:0000250|UniProtKB:P07902"
FT BINDING 165
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10033"
FT BINDING 169
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /ligand_note="ligand shared between dimeric partners"
FT /note="in other chain"
FT /evidence="ECO:0000250|UniProtKB:P07902"
FT BINDING 315..318
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /ligand_note="ligand shared between dimeric partners"
FT /note="in other chain"
FT /evidence="ECO:0000250|UniProtKB:P07902"
FT BINDING 320..321
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /ligand_note="ligand shared between dimeric partners"
FT /note="in other chain"
FT /evidence="ECO:0000250|UniProtKB:P07902"
SQ SEQUENCE 352 AA; 41043 MW; F1B433A926C47C4C CRC64;
MSKQNNFRRY NPLIDEWVIV AVNRINRPWQ GAKTEKSTTI STSSNTEQSI LNPLAPGGTR
SSGIANENYV STYVFDNDFP SFTEFEECAG KDENDDLFKQ HEVKGVCKVI CYHPNSQLTL
ATMDVKEVRV VIDIWNQQYL ELGPKYEWVQ IFENRGAVVG CSNMHPHGQL WASNYLPTLP
MKKHESQKKH FEKHGKVMLM DYLEQETLKK ERIIMRNEHW TWLVPYWAFW PYETMLLPNR
HVERFTDLGE VEKQSLSEIL RSLLIKYDNV FECSFPYMMG WSGAPTGSFL TENCSFWQLH
LSFFPPLLRS ATVPKFLAGY EVFAEKQRDL SPEIAAKTLS EIDEEHYSKK LQ
