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GALT_DROME
ID   GALT_DROME              Reviewed;         350 AA.
AC   Q9VMA2; B9EQS8; Q8SXN7;
DT   30-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 2.
DT   03-AUG-2022, entry version 162.
DE   RecName: Full=Probable galactose-1-phosphate uridylyltransferase;
DE            Short=Gal-1-P uridylyltransferase;
DE            EC=2.7.7.12 {ECO:0000250|UniProtKB:P09148};
DE   AltName: Full=UDP-glucose--hexose-1-phosphate uridylyltransferase;
GN   Name=Galt; ORFNames=CG9232;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley; TISSUE=Head;
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA   Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA   Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley;
RA   Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.;
RL   Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-D-galactose 1-phosphate + UDP-alpha-D-glucose = alpha-D-
CC         glucose 1-phosphate + UDP-alpha-D-galactose; Xref=Rhea:RHEA:13989,
CC         ChEBI:CHEBI:58336, ChEBI:CHEBI:58601, ChEBI:CHEBI:58885,
CC         ChEBI:CHEBI:66914; EC=2.7.7.12;
CC         Evidence={ECO:0000250|UniProtKB:P09148};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000250|UniProtKB:P09148};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000250|UniProtKB:P09148};
CC   -!- PATHWAY: Carbohydrate metabolism; galactose metabolism.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P07902}.
CC   -!- SIMILARITY: Belongs to the galactose-1-phosphate uridylyltransferase
CC       type 1 family. {ECO:0000305}.
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DR   EMBL; AE014134; AAF52419.2; -; Genomic_DNA.
DR   EMBL; AY089524; AAL90262.1; -; mRNA.
DR   EMBL; BT058003; ACM16713.1; -; mRNA.
DR   RefSeq; NP_001260142.1; NM_001273213.1.
DR   RefSeq; NP_609062.2; NM_135218.3.
DR   AlphaFoldDB; Q9VMA2; -.
DR   SMR; Q9VMA2; -.
DR   BioGRID; 60089; 9.
DR   DIP; DIP-18538N; -.
DR   IntAct; Q9VMA2; 1.
DR   STRING; 7227.FBpp0078951; -.
DR   PaxDb; Q9VMA2; -.
DR   PRIDE; Q9VMA2; -.
DR   DNASU; 33935; -.
DR   EnsemblMetazoa; FBtr0079322; FBpp0078951; FBgn0263200.
DR   EnsemblMetazoa; FBtr0333352; FBpp0305544; FBgn0263200.
DR   GeneID; 33935; -.
DR   KEGG; dme:Dmel_CG9232; -.
DR   UCSC; CG9232-RA; d. melanogaster.
DR   CTD; 2592; -.
DR   FlyBase; FBgn0263200; Galt.
DR   VEuPathDB; VectorBase:FBgn0263200; -.
DR   eggNOG; KOG2958; Eukaryota.
DR   GeneTree; ENSGT00390000016188; -.
DR   HOGENOM; CLU_029960_0_0_1; -.
DR   InParanoid; Q9VMA2; -.
DR   OMA; HAIYYPP; -.
DR   OrthoDB; 1135699at2759; -.
DR   PhylomeDB; Q9VMA2; -.
DR   Reactome; R-DME-70370; Galactose catabolism.
DR   UniPathway; UPA00214; -.
DR   BioGRID-ORCS; 33935; 0 hits in 1 CRISPR screen.
DR   GenomeRNAi; 33935; -.
DR   PRO; PR:Q9VMA2; -.
DR   Proteomes; UP000000803; Chromosome 2L.
DR   Bgee; FBgn0263200; Expressed in adult Malpighian tubule (Drosophila) and 34 other tissues.
DR   ExpressionAtlas; Q9VMA2; baseline and differential.
DR   Genevisible; Q9VMA2; DM.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0008108; F:UDP-glucose:hexose-1-phosphate uridylyltransferase activity; IMP:FlyBase.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0008344; P:adult locomotory behavior; IMP:FlyBase.
DR   GO; GO:0033499; P:galactose catabolic process via UDP-galactose; IBA:GO_Central.
DR   GO; GO:0033501; P:galactose homeostasis; IMP:FlyBase.
DR   CDD; cd00608; GalT; 1.
DR   Gene3D; 3.30.428.10; -; 2.
DR   InterPro; IPR001937; GalP_UDPtransf1.
DR   InterPro; IPR019779; GalP_UDPtransf1_His-AS.
DR   InterPro; IPR005850; GalP_Utransf_C.
DR   InterPro; IPR005849; GalP_Utransf_N.
DR   InterPro; IPR036265; HIT-like_sf.
DR   PANTHER; PTHR11943; PTHR11943; 1.
DR   Pfam; PF02744; GalP_UDP_tr_C; 1.
DR   Pfam; PF01087; GalP_UDP_transf; 1.
DR   PIRSF; PIRSF000808; GalT; 1.
DR   SUPFAM; SSF54197; SSF54197; 2.
DR   TIGRFAMs; TIGR00209; galT_1; 1.
DR   PROSITE; PS00117; GAL_P_UDP_TRANSF_I; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Galactose metabolism; Metal-binding;
KW   Nucleotidyltransferase; Reference proteome; Transferase; Zinc.
FT   CHAIN           1..350
FT                   /note="Probable galactose-1-phosphate uridylyltransferase"
FT                   /id="PRO_0000169886"
FT   REGION          31..52
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        165
FT                   /note="Tele-UMP-histidine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10033"
FT   BINDING         54
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10033"
FT   BINDING         76..77
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /note="in other chain"
FT                   /evidence="ECO:0000250|UniProtKB:P07902"
FT   BINDING         152
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /evidence="ECO:0000250|UniProtKB:P07902"
FT   BINDING         163
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10033"
FT   BINDING         167
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /note="in other chain"
FT                   /evidence="ECO:0000250|UniProtKB:P07902"
FT   BINDING         314..317
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /note="in other chain"
FT                   /evidence="ECO:0000250|UniProtKB:P07902"
FT   BINDING         319..320
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /note="in other chain"
FT                   /evidence="ECO:0000250|UniProtKB:P07902"
FT   CONFLICT        305
FT                   /note="P -> T (in Ref. 3; AAL90262)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   350 AA;  40447 MW;  C946229E44C2B6AD CRC64;
     MQFVASEHPH RRLNPLNGQW VLVCPHRTQR PWSGQQEKAQ KNELPEFDPT NPLCPGVTRP
     NGIQTPEYES TYVFENDFPA LVEVVPVPPN NDDPLFQIAP ARGNCRVMCF HPKSNLTLPT
     MSAAEIVVVI DEWISQFNEL SAKYAWVQIF ENKGAAMGCS NPHPHCQIWS CSFLPTEPQL
     KQERLRAYYA TNERPMLADY VERELQRQER IVIENRDWLV VVPFWATWPF ETMLISRNNN
     KRINDLTAEQ RYNLALTIKE LTTKYDNLFQ CSFPYSMGWH GAPTGPEHAH ASSAHWTLHA
     IYYPPLLRSA SVRKFMVGFE LLAMAQRDLT PEQAAQRLRE VDGKCHYLEK
 
 
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