ID   ALGI_AZOVI              Reviewed;         499 AA.
AC   O52196;
DT   25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Probable alginate O-acetylase AlgI;
DE            EC=2.3.1.-;
DE   AltName: Full=Alginate biosynthesis protein AlgI;
GN   Name=algI;
OS   Azotobacter vinelandii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
OX   NCBI_TaxID=354;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 9046;
RX   PubMed=10352233; DOI=10.1016/s0378-1119(99)00119-5;
RA   Vazquez-Ramos A., Moreno S., Guzman J., Alvarado A., Espin G.;
RT   "Transcriptional organization of the Azotobacter vinelandii algGXLVIFA
RT   genes: characterization of algF mutants.";
RL   Gene 232:217-222(1999).
CC   -!- FUNCTION: Together with AlgJ and AlgF, forms an inner membrane complex
CC       which probably interacts with the alginate polymerization-transport
CC       complex and adds acetyl groups at the O-2 and O-3 positions of
CC       mannuronate residues. Acetylation of alginate increases cyst resistance
CC       to desiccation.
CC   -!- PATHWAY: Glycan biosynthesis; alginate biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the membrane-bound acyltransferase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF027499; AAC04568.1; -; Genomic_DNA.
DR   RefSeq; WP_012699741.1; NZ_FPKM01000036.1.
DR   AlphaFoldDB; O52196; -.
DR   SMR; O52196; -.
DR   OMA; IIWGAWH; -.
DR   UniPathway; UPA00286; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0042121; P:alginic acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR024194; Ac/AlaTfrase_AlgI/DltB.
DR   InterPro; IPR028362; AlgI.
DR   InterPro; IPR004299; MBOAT_fam.
DR   Pfam; PF03062; MBOAT; 1.
DR   PIRSF; PIRSF500217; AlgI; 1.
DR   PIRSF; PIRSF016636; AlgI_DltB; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Alginate biosynthesis; Cell inner membrane; Cell membrane;
KW   Membrane; Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..499
FT                   /note="Probable alginate O-acetylase AlgI"
FT                   /id="PRO_0000213123"
FT   TRANSMEM        7..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        40..62
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        78..100
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        115..137
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        150..172
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        239..261
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        312..334
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        354..373
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        380..397
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        407..429
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        475..497
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        322
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   499 AA;  56387 MW;  CBB74AE4DF08F63C CRC64;
     MVFSSNVFLF LFLPLFLGLY YLSPARYRNL LLLTASYVFY AWWRIDFLGL FAAVTVFNYW
     IGLRIGAAGV RTQSAQRWLT LGVVVDLCVL GYFKYANFGV DSLNAIITSF GVEPFVVTHI
     LLPIGISFYV FESISYIIDV YRGDTPATRN LVDFAAFVAI FPHLIAGPVL RFRDLVDQFN
     HRTHTLDKFA EGCTRFMQGF IKKVFIADSL APIADHCFAL SDPTTGDAWL GALAYTAQLY
     FDFSGYSDMA IGLGLMIGFR FMENFNQPYI SQSITEFWRR WHISLSTWLR DYLYISLGGN
     RGTTFQTYRN LILTMLLGGL WHGANWTFII WGAWHGTWLA IERALRIDAA PKTIRPLRWV
     FAFLLVMVGW VIFRAENLDV AWRMYAAMFS FGDWTLSDLN KAQLTSLQIA TLLLAYVVIA
     VYGIRQFYAQ PLTGAPKAKA NDQADAPQGI VHASSGTLAY SQAIDIPALA TRVAVLLLFA
     ASVLKLSAQS FSPFLYFQF