ID   GAM_BPMU                Reviewed;         174 AA.
AC   P06023;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 2.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Putative DNA ends protecting protein gam;
DE   AltName: Full=Gene product 10;
DE            Short=gp10;
GN   Name=gam; OrderedLocusNames=Mup10;
OS   Escherichia phage Mu (Bacteriophage Mu).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Muvirus.
OX   NCBI_TaxID=10677;
OH   NCBI_TaxID=543; Enterobacteriaceae.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2945162; DOI=10.1093/nar/14.17.6901;
RA   Akroyd J.E., Clayson E., Higgins N.P.;
RT   "Purification of the gam gene-product of bacteriophage Mu and determination
RT   of the nucleotide sequence of the gam gene.";
RL   Nucleic Acids Res. 14:6901-6914(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Priess H., Brauer B., Schmidt C., Kamp D.;
RT   "Sequence of the left end of Mu.";
RL   (In) Symonds N., Toussaint A., van de Putte P., Howe M.M. (eds.);
RL   Phage Mu, pp.277-296, Cold Spring Harbor Laboratory Press, New York (1987).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11922669; DOI=10.1006/jmbi.2002.5437;
RA   Morgan G.J., Hatfull G.F., Casjens S., Hendrix R.W.;
RT   "Bacteriophage Mu genome sequence: analysis and comparison with Mu-like
RT   prophages in Haemophilus, Neisseria and Deinococcus.";
RL   J. Mol. Biol. 317:337-359(2002).
RN   [4]
RP   IDENTIFICATION.
RX   PubMed=2952555; DOI=10.1016/0378-1119(86)90288-x;
RA   Akroyd J., Symonds N.;
RT   "Localization of the gam gene of bacteriophage mu and characterisation of
RT   the gene product.";
RL   Gene 49:273-282(1986).
RN   [5]
RP   INDUCTION.
RX   PubMed=2524470; DOI=10.1128/jb.171.6.3440-3448.1989;
RA   Stoddard S.F., Howe M.M.;
RT   "Localization and regulation of bacteriophage Mu promoters.";
RL   J. Bacteriol. 171:3440-3448(1989).
RN   [6]
RP   FUNCTION, DNA-BINDING, AND SUBUNIT.
RX   PubMed=12524520; DOI=10.1038/sj.embor.embor709;
RA   d'Adda di Fagagna F., Weller G.R., Doherty A.J., Jackson S.P.;
RT   "The Gam protein of bacteriophage Mu is an orthologue of eukaryotic Ku.";
RL   EMBO Rep. 4:47-52(2003).
CC   -!- FUNCTION: Protects linear double-stranded DNA from host exonuclease
CC       degradation. Binds to linear dsDNA irrespective of the precise
CC       architecture of the DNA terminus but not to RNA or to ssDNA.
CC       {ECO:0000269|PubMed:12524520}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:12524520}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000305}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       Expression of early genes is repressed by viral Repc (latency) and
CC       favored by viral Ner protein. {ECO:0000269|PubMed:2524470}.
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DR   EMBL; X04390; CAA27978.1; -; Genomic_DNA.
DR   EMBL; M64097; AAA32394.1; -; Genomic_DNA.
DR   EMBL; AF083977; AAF01087.1; -; Genomic_DNA.
DR   PIR; A24331; DNBPMU.
DR   RefSeq; NP_050614.1; NC_000929.1.
DR   SMR; P06023; -.
DR   GeneID; 2636288; -.
DR   KEGG; vg:2636288; -.
DR   Proteomes; UP000002611; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003690; F:double-stranded DNA binding; IEA:InterPro.
DR   GO; GO:0042262; P:DNA protection; IEA:InterPro.
DR   GO; GO:0099016; P:evasion by virus of DNA end degradation; IEA:UniProtKB-KW.
DR   InterPro; IPR009951; Host-nuc_inhib_Gam.
DR   Pfam; PF07352; Phage_Mu_Gam; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; DNA end degradation evasion by virus; DNA-binding;
KW   Early protein; Host cytoplasm; Host-virus interaction; Reference proteome.
FT   CHAIN           1..174
FT                   /note="Putative DNA ends protecting protein gam"
FT                   /id="PRO_0000077639"
FT   COILED          26..56
FT                   /evidence="ECO:0000255"
FT   CONFLICT        130
FT                   /note="I -> V (in Ref. 1; CAA27978)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   174 AA;  19046 MW;  804B73114682A6ED CRC64;
     MAKPAKRIKS AAAAYVPQNR DAVITDIKRI GDLQREASRL ETEMNDAIAE ITEKFAARIA
     PIKTDIETLS KGVQGWCEAN RDELTNGGKV KTANLVTGDV SWRVRPPSVS IRGMDAVMET
     LERLGLQRFI RTKQEINKEA ILLEPKAVAG VAGITVKSGI EDFSIIPFEQ EAGI