ID   GANR_BACSU              Reviewed;         330 AA.
AC   O07008; Q795J6;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=HTH-type transcriptional regulator GanR {ECO:0000305};
GN   Name=ganR {ECO:0000303|PubMed:27501980};
GN   Synonyms=lacR {ECO:0000303|PubMed:2104611}, yvfJ;
GN   OrderedLocusNames=BSU34170;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX   PubMed=9287030; DOI=10.1128/jb.179.17.5636-5638.1997;
RA   Daniel R.A., Haiech J., Denizot F., Errington J.;
RT   "Isolation and characterization of the lacA gene encoding beta-
RT   galactosidase in Bacillus subtilis and a regulator gene, lacR.";
RL   J. Bacteriol. 179:5636-5638(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Denizot F.;
RL   Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   FUNCTION.
RC   STRAIN=168;
RX   PubMed=2104611; DOI=10.1128/jb.172.1.488-490.1990;
RA   Errington J., Vogt C.H.;
RT   "Isolation and characterization of mutations in the gene encoding an
RT   endogenous Bacillus subtilis beta-galactosidase and its regulator.";
RL   J. Bacteriol. 172:488-490(1990).
RN   [5]
RP   FUNCTION, AND DNA-BINDING.
RX   PubMed=27501980; DOI=10.1128/jb.00468-16;
RA   Watzlawick H., Morabbi Heravi K., Altenbuchner J.;
RT   "Role of the ganSPQAB operon in degradation of galactan by Bacillus
RT   subtilis.";
RL   J. Bacteriol. 198:2887-2896(2016).
CC   -!- FUNCTION: Negatively regulates the expression of the ganSPQAB operon
CC       (PubMed:2104611, PubMed:9287030, PubMed:27501980). Inhibits
CC       transcription of the operon by binding to an operator in the promoter
CC       region (PubMed:27501980). In the presence of galactobiose, GanR
CC       dissociates from the promoter, resulting in the expression of the gan
CC       operon (PubMed:27501980). {ECO:0000269|PubMed:2104611,
CC       ECO:0000269|PubMed:27501980, ECO:0000269|PubMed:9287030}.
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DR   EMBL; Z94043; CAB08004.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB15422.1; -; Genomic_DNA.
DR   PIR; C69649; C69649.
DR   RefSeq; NP_391297.1; NC_000964.3.
DR   RefSeq; WP_003244199.1; NZ_JNCM01000033.1.
DR   AlphaFoldDB; O07008; -.
DR   SMR; O07008; -.
DR   STRING; 224308.BSU34170; -.
DR   PaxDb; O07008; -.
DR   PRIDE; O07008; -.
DR   EnsemblBacteria; CAB15422; CAB15422; BSU_34170.
DR   GeneID; 936331; -.
DR   KEGG; bsu:BSU34170; -.
DR   PATRIC; fig|224308.179.peg.3704; -.
DR   eggNOG; COG1609; Bacteria.
DR   OMA; NPAPELF; -.
DR   PhylomeDB; O07008; -.
DR   BioCyc; BSUB:BSU34170-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR   CDD; cd01392; HTH_LacI; 1.
DR   Gene3D; 1.10.260.40; -; 1.
DR   InterPro; IPR000843; HTH_LacI.
DR   InterPro; IPR046335; LacI/GalR-like_sensor.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR028082; Peripla_BP_I.
DR   Pfam; PF00356; LacI; 1.
DR   Pfam; PF13377; Peripla_BP_3; 1.
DR   SMART; SM00354; HTH_LACI; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   SUPFAM; SSF53822; SSF53822; 1.
DR   PROSITE; PS50932; HTH_LACI_2; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..330
FT                   /note="HTH-type transcriptional regulator GanR"
FT                   /id="PRO_0000381931"
FT   DOMAIN          2..57
FT                   /note="HTH lacI-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00111"
FT   DNA_BIND        4..23
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00111"
SQ   SEQUENCE   330 AA;  37462 MW;  12285A5EB181D4BF CRC64;
     MATIKDIAQE AGFSISTVSR VLNNDESLSV PDETREKIYE AAEKLNYRKK TVRPLVKHIA
     FLYWLTDKEE LEDVYFKTMR LEVEKLAKAF NVDMTTYKIA DGIESIPEHT EGFIAVGTFS
     DEELAFLRNL TENGVFIDST PDPDHFDSVR PDLAQMTRKT VNILTEKGHK SIGFIGGTYK
     NPNTNQDEMD IREQTFRSYM REKAMLDERY IFCHRGFSVE NGYRLMSAAI DTLGDQLPTA
     FMIAADPIAV GCLQALNEKG IAIPNRVSIV SINNISFAKY VSPPLTTFHI DIHELCKNAV
     QLLLEQVQDK RRTVKTLYVG AELIVRKSMN